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Specific SNP molecular marker for identifying cabbage clubroot 4# physiological race resistance and application

A technology for Chinese cabbage clubroot and Chinese cabbage, which is applied to the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., can solve problems such as unsatisfactory control effects, achieve accurate and reliable identification results, and speed up Chinese cabbage. Effect of anti-clubroot breeding process and labor cost reduction

Active Publication Date: 2016-04-27
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although clubroot can be controlled by chemical agents and enhanced cultivation management, the control effect is not satisfactory

Method used

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  • Specific SNP molecular marker for identifying cabbage clubroot 4# physiological race resistance and application
  • Specific SNP molecular marker for identifying cabbage clubroot 4# physiological race resistance and application
  • Specific SNP molecular marker for identifying cabbage clubroot 4# physiological race resistance and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1, the acquisition of molecular markers

[0075] In order to screen and obtain clubroot resistance resources better and faster, and to promote the practice of molecular marker-assisted selection breeding, through the QTL mapping of the DH population composed of 68 lines combined with the clubroot phenotype, obtained on the A02 chromosome A major QTL closely linked to clubroot resistance was identified. Further design and screen SNP markers in this interval, and finally find that there is a SNP site with a G / T mutation at base 20509373 on chromosome A2 (nucleotide 301 in Sequence Listing 4), the SNP site Named A0220509373G / T site. According to the A0220509373G / TSNP site, the following specific primers that can be used in KASP technology were designed as molecular markers: upstream primer A0220509373-FF, upstream primer A0220509373-FV and downstream primer A0220509373-R.

[0076] Above-mentioned A0220509373-FF, A0220509373-FV and A0220509373-R primers were entru...

Embodiment 2

[0085] Example 2, Application of A0220509373 Molecular Marker in Identification of Chinese Cabbage Clubroot No. 4 Physiological Race Resistance

[0086] 1. Molecular marker identification of Chinese cabbage clubroot and Chinese cabbage clubroot materials

[0087] 1) DNA extraction

[0088] The genomic DNA of the 68 DH populations in Table 1 was extracted by conventional CTAB method.

[0089] The quality of the extracted DNA was detected by agarose electrophoresis and Nanodrop2100 respectively, and it was found that the extracted genomic DNA met the relevant quality requirements, that is, the agarose electrophoresis showed a single DNA band without obvious dispersion; the A260 / 280 detected by Nanodrop2100 was between 1.8- Between 2.0 (DNA sample without protein contamination); A260 / 230 between 1.8-2.0 (DNA sample with low salt ion concentration); no obvious light absorption at 270nm (DNA sample without phenol contamination); for competitive allele The amount of DNA detected b...

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Abstract

The invention discloses a specific SNP (Single Nucleotide Polymorphism) molecular marker for identifying cabbage clubroot 4# physiological race resistance and application. The invention provides application of an A0220509373G / T site in any one aspect as follows: A, application to identification or auxiliary identification of cabbage clubroot resistance; B, application to breeding of cabbage-clubroot-resistant varieties; C, application to cabbage breeding; D, application to prediction of cabbage clubroot resistance; E, application to preparing a product for identification or auxiliary identification of cabbage clubroot resistance; and F, application to preparation of a product for predicting cabbage clubroot resistance. Experiments prove that the A0220509373G / T site and a specific primer of the A0220509373G / T site can realize good typing on the cabbage clubroot; a good application value is realized; and the selection in advance and the auxiliary breeding on materials resisting the clubroot inheritance can be realized.

Description

technical field [0001] The invention relates to a SNP molecular marker for identifying Chinese cabbage clubroot resistance in the field of biotechnology, in particular to a SNP molecular marker for identifying Chinese cabbage clubroot resistance No. 4 physiological race and application thereof. Background technique [0002] Cruciferous vegetable clubroot is a worldwide disease caused by Plasmodiophora brassicae Woron. Phizobia obligately parasitizes the roots of cruciferous plants. When it infects the roots, the parenchyma cells divide and enlarge in large numbers, thus forming tumors. The aboveground part shows symptoms such as leaf chlorosis, dullness, yellowing of leaf margin, slow growth, short plant, wilting, etc., which can lead to crop failure in severe cases. According to statistics, clubroot causes losses of more than 10% of cruciferous crops in the world. [0003] Clubroot was first discovered in the Mediterranean coast (UK, 1737) and southern Europe (Leningrad, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 苏同兵汪维红于拴仓张凤兰余阳俊张德双赵岫云卢桂香
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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