Water-blocking microcapsule and fish oil composite hair beautifying ointment and preparation method thereof

Abstract

The invention provides a water-blocking microcapsule, a fish oil composite hair beautifying ointment and a preparation method, and relates to the technical field of animal health products. The water-blocking microcapsule is a solid particle formed by a core material and a wall material coating the core material; the core material is fish oil emulsion subjected to bile salt emulsification treatment; the wall material sequentially comprises an inner-layer wall material and an outer-layer wall material from inside to outside; the inner-layer wall material is a wall material formed by solidifying alginate; and the outer-layer wall material is a water-blocking wall material which is arranged outside the inner-layer wall material and is made of nanocrystalline cellulose. The water-blocking microcapsule provided by the invention realizes multiple beneficial effects of efficient digestion and absorption of fish oil, improvement of palatability, enhancement of oxidation resistance and stability, fixed-point release of intestinal tracts and the like, provides an innovative technical scheme for development of animal health products, and has wide application prospects and market values.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of animal health products, in particular, it relates to a water-blocking microcapsule, a fish oil compound hair beautifying cream and a preparation method. BACKGROUND

[0002] With the development of society and the improvement of people's living standards, animal health has become a widely concerned topic. Whether it is a pet such as a cat or a dog, or a livestock such as a cow, a sheep, or a pig, which has important economic value in the livestock industry, their health status directly affects the quality of human life and economic benefits. Animal health not only includes its physiological health, but also involves the health of appearance, such as the luster, softness and growth of hair. These appearance characteristics not only reflect the overall health status of the animal, but also enhance the ornamental value and market value of the animal. Therefore, nutrition products for animal hair health have emerged to meet the dual needs of people for animal health and appearance.

[0003] Among many animal health products, hair beautifying functional nutrition occupies an important position. Such products usually take fish oil rich in Omega-3 as the main ingredient, because Omega-3 fatty acids have significant effects on promoting animal hair growth and improving hair quality. However, although fish oil has advantages in hair beautifying, it still has some problems in practical application. For example, fish oil has poor palatability, and its strong fishy smell often makes it difficult for animals to accept, resulting in many inconveniences for animal owners in the use process. In addition, fish oil is prone to oxidation and spoilage during storage and use, producing unpleasant odors, further affecting the palatability and safety of the product.

[0004] In addition to the problem of palatability, existing animal hair beautifying creams also have deficiencies in hair beautifying effect. Many products claim to contain hair beautifying ingredients such as fish oil, but the content of Omega-3, the core functional ingredient, often cannot achieve the ideal hair beautifying effect. Some products do not even have a clear indication of the content of Omega-3, making it difficult for consumers to judge the efficacy of the product. This opacity of information not only affects the choice of consumers, but also makes it difficult to guarantee the actual effect of the product. For the livestock industry, this uncertainty may also lead to ineffective improvement of the hair health of livestock, thereby affecting their production performance and market value.

[0005] Furthermore, some animal hair beautifying creams have flawed formulations, resulting in poor product stability. To enhance their coat-beautifying effects, some products increase the content of omega-3-rich oils. However, excessively high oil content can easily lead to an imbalanced oil-to-water ratio and unstable emulsion systems. This unstable state causes the paste to leak oil, resulting in poor formability and stability. Animals may experience indigestion and malabsorption after use. Furthermore, the demulsification and oil release also expose fatty acids to air, accelerating oxidation and rancidity, further affecting the product's palatability and safety. For pet owners, this can lead to health issues and inconvenience in use; for the livestock industry, it can result in reduced livestock performance and economic losses.

[0006] In summary, existing animal hair beautification creams suffer from numerous deficiencies in palatability, hair beautification efficacy, and product stability. These issues not only impact the user experience and effectiveness of the products but also pose a potential threat to the health and well-being of animals. Therefore, developing an animal hair beautification product that effectively addresses these issues is of great practical significance and holds broad market potential.

[0007] In view of this, the present invention is proposed. Summary of the Invention

[0008] The purpose of the present invention is to provide a water-blocking microcapsule, a fish oil composite hair-beautifying cream and a preparation method thereof. The water-blocking microcapsule has the advantages of improving palatability and emulsification system stability, and ensuring hair-beautifying effects.

[0009] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are adopted: In a first aspect, the present invention provides a water-blocking microcapsule, which is a solid particle composed of a core material and a wall material coated on the outside of the core material; wherein the core material is a fish oil emulsion emulsified with bile salts; the wall materials are, from the inside to the outside, an inner wall material and an outer wall material; the inner wall material is a wall material composed of solidified alginate; and the outer wall material is a water-blocking wall material composed of nanocrystalline cellulose and arranged outside the inner wall material.

[0010] In a second aspect, the present application provides a method for preparing the water-resistant microcapsule as described in the preceding embodiments, comprising: emulsifying concentrated fish oil with a bile salt to obtain an O / W / O emulsion core material; mixing the O / W / O emulsion core material with a whey protein aqueous solution and an alginate to obtain a whey mixture solution; adding the whey mixture solution dropwise into a first calcium lactogluconate solution, and after a first solidification treatment, obtaining a single-layer microcapsule coated with an inner wall material; adding the single-layer microcapsule into a nanocrystalline cellulose solution, and after a second solidification treatment, obtaining a double-layer microcapsule; dispersing the double-layer microcapsule into a second calcium lactogluconate solution, and after a third solidification treatment, a sodium chloride solution cleaning treatment, and a freeze-drying treatment, obtaining the water-resistant microcapsule. In a preferred embodiment, the first calcium lactogluconate solution is a calcium lactogluconate solution with a concentration of 5% w / v to 10% w / v and a pH of 3 to 5. In a preferred embodiment, the second calcium lactogluconate solution is a calcium lactogluconate solution with a concentration of 1% w / v to 3% w / v and a pH of 1 to 3. In a preferred embodiment, the weight ratio of the O / W / O emulsion core material, the alginate, and the whey protein aqueous solution is (1 to 2):(4 to 5):(4 to 6). In a preferred embodiment, the mass concentration of the whey protein aqueous solution is 15.0 g / L to 40.0 g / L. In a preferred embodiment, the mass concentration of the alginate aqueous solution is 15.0 g / L to 40.0 g / L. In a preferred embodiment, the first solidification treatment has a treatment time of 20 minutes to 50 minutes. In a preferred embodiment, the mass concentration of the nanocrystalline cellulose solution is 0.3% to 2%. In a preferred embodiment, the second solidification treatment has a treatment time of 2 minutes to 10 minutes. In a preferred embodiment, the sodium chloride solution cleaning treatment is cleaning with a sodium chloride solution with a mass concentration of 0.5% to 1.5% to a neutral pH.

[0011] In a preferred embodiment, after the step of adding the whey mixture solution dropwise into the first calcium lactogluconate solution and after the first solidification treatment to obtain the single-layer microcapsule coated with the inner wall material, the method further comprises: cleaning the single-layer microcapsule with a sodium chloride solution with a mass concentration of 0.5% to 1.5% to a neutral pH to obtain the cleaned single-layer microcapsule.

[0012] In a preferred embodiment, the whey protein aqueous solution is prepared by adding whey protein to water and continuously stirring for 1 to 2 hours; centrifuging at 3000 rpm to 8000 rpm for 10 to 15 minutes, and taking the supernatant as the whey protein aqueous solution.

[0013] In a preferred embodiment, before the concentrated fish oil is emulsified with bile salts to obtain the O / W / O type emulsion core material, the process further comprises: separately preparing an inner oil phase mixture, an aqueous phase mixture, and an outer oil phase mixture; In a preferred embodiment, the internal oil phase mixture includes the following components in parts by weight: The first oil component has 15-20 grams; contains 1-4 grams of a bile salt emulsifier; Wherein, the first oil component is concentrated fish oil containing no less than 50% of Omega 3; In a preferred embodiment, the external oil phase mixture includes the following components in parts by weight: Second oil component 25~45; W / O emulsifier 1~10; Wherein, the second oil component is concentrated fish oil containing not less than 50% of Omega 3; In a preferred embodiment, the aqueous phase mixture comprises the following components in parts by weight: O / W emulsifier 1~10; co-emulsifier 2~5; water 25~45; In a preferred embodiment, the bile salt containing bile salt emulsifier in the internal oil phase mixture includes taurocholate sodium salt and / or glycinecholate sodium salt; In a preferred embodiment, the O / W emulsifier in the aqueous phase mixture is a hydrophilic emulsifier; In a preferred embodiment, the O / W emulsifier in the aqueous phase mixture includes at least one of Tween 20, Tween 40, Tween 60 and Tween 80; In a preferred embodiment, the W / O emulsifier in the external oil phase mixture is a lipophilic emulsifier; In a preferred embodiment, the W / O emulsifier in the external oil phase mixture includes at least one of Span 20, Span 40, Span 60, and Span 80; In a preferred embodiment, the co-emulsifier includes n-butanol, ethylene glycol, ethanol, propylene glycol, glycerol and polyglycerol esters; In a preferred embodiment, the weight ratio of the inner oil phase mixture, the water phase mixture and the outer oil phase mixture is (1-2):(1-4):(1-2).

[0014] In a preferred embodiment, the concentrated fish oil is emulsified using bile salts to obtain an O / W / O type emulsion core material, comprising: Preheating the inner oil phase mixture, the water phase mixture, and the outer oil phase mixture respectively; Stirring the aqueous phase mixture, adding the inner oil phase mixture, and ultrasonically treating to obtain an O / W mixed emulsion; adding the outer oil phase mixture to the O / W mixed emulsion, and stirring to obtain the O / W / O type emulsion core material; In a preferred embodiment, the temperature of the preheating treatment is not less than 85°C; In a preferred embodiment, the step is to stir the aqueous phase mixture and add the inner oil phase mixture, and after ultrasonic treatment, obtain an O / W mixed emulsion, and the stirring rate is 500 r / min.

[0015] In a preferred embodiment, the step comprises adding the external oil phase mixture to the O / W mixed emulsion, and stirring to obtain the O / W / O type emulsion core material, wherein the stirring treatment comprises: first performing a first stirring at a stirring rate of 650 r / min; stirring for no less than 30 minutes; then performing a second stirring at a stirring rate of 580 r / min; and continuing stirring until cooling to room temperature.

[0016] In a third aspect, the present invention provides a fish oil compound hair-beautifying cream, which is a composite cream composed of the water-blocking microcapsules described in the aforementioned embodiment, as well as an oil component, a surfactant, a protein component, an amino acid component, a mineral component, a vitamin mixture, an organic acid component, a thickener, a carbohydrate substance and water.

[0017] In a preferred embodiment, the oil component comprises at least one of fish oil, seal oil, Antarctic krill oil and algae oil; In a preferred embodiment, the surfactant comprises at least one of egg yolk powder, soy lecithin and egg yolk lecithin; In a preferred embodiment, the protein component comprises at least one of bone gelatin, milk powder, yeast extract, chicken hydrolysate and porcine plasma protein; In a preferred embodiment, the mineral component includes copper and / or zinc; In a preferred embodiment, the mineral component exists in the form of amino acid complexes and / or oxides; In a preferred embodiment, the mineral components are copper and zinc, wherein the weight ratio of copper to zinc is (0.01-1.5):(0.01-3.5); In a preferred embodiment, the amino acid component comprises L-cysteine and / or L-tyrosine; In a preferred embodiment, the amino acid component comprises L-cysteine and L-tyrosine; wherein the weight ratio of L-cysteine to L-tyrosine is (0.05~5):(0.05~5). In a preferred embodiment, the vitamin mixture comprises at least one of vitamin B1, vitamin B2, vitamin B12 and vitamin E. In a preferred embodiment, the vitamin mixture comprises vitamin B1, vitamin B2, vitamin B12 and vitamin E; wherein the weight ratio of vitamin B1 to vitamin B2 to vitamin B12 to vitamin E is (0.01~1):(0.05~1):(0.05~1):(0.05~1). In a preferred embodiment, the organic acid comprises citric acid and / or lactic acid. In a preferred embodiment, the sugar substance comprises monosaccharide and / or disaccharide. In a preferred embodiment, the thickening agent comprises at least one of gelatin, sodium carboxymethyl cellulose, xanthan gum, pectin, gellan gum and locust bean gum.

[0018] In a preferred embodiment, the fish oil compound hair conditioner comprises the following components in the following weight ratio: Water-resistant microcapsule 10~40; oil and fat component 10~40; surfactant 0.2~7; protein component 15~20; amino acid component 0.5~5; mineral component 0.1~0.8; vitamin mixture 0.1~1; organic acid component 1~10; thickening agent 1~10; sugar substance 5~20; water 5~55.

[0019] In a fourth aspect, the present application provides a preparation method of the fish oil compound hair conditioner according to any one of the preceding embodiments, comprising: mixing water and carrier substance to obtain a carrier solution; stirring and mixing water, protein component, amino acid component, vitamin mixture, organic acid component and sugar substance in the carrier solution to obtain a nutrient mixture; homogenously stirring and heating oil and fat component and surfactant to 65~70℃ to obtain a homogenously mixed oil; homogenously emulsifying the nutrient mixture by adding a thickening agent, and then adding the homogenously mixed oil to obtain an emulsified mixture; heating the emulsified mixture to 70℃ during stirring, adding water-resistant microcapsule, and continuing to stir and boil; cooling to room temperature to obtain the fish oil compound hair conditioner.

[0020] The present invention provides a water-blocking microcapsule, a fish oil composite hair-beautifying cream, and a preparation method. The water-blocking microcapsule is a solid particle composed of a core material and a wall material coated on the outside of the core material; the core material is a fish oil emulsion emulsified with bile salts; the wall materials are, from the inside to the outside, an inner wall material and an outer wall material; the inner wall material is a wall material composed of solidified alginate; and the outer wall material is a water-blocking wall material composed of nanocrystalline cellulose and disposed outside the inner wall material. Compared with the prior art, the water-blocking microcapsules provided by the present invention have the following beneficial effects: (1) Improve the digestion and absorption rate of fish oil: By emulsifying fish oil with bile salts to form an emulsion as the core material of microcapsules, the particle size of fish oil can be effectively reduced, and its contact area with digestive enzymes can be increased, thereby improving the digestion and absorption rate of fish oil in animals. Bile salts, as natural emulsifiers, can combine with fatty acids in fish oil to form water-soluble complexes, further promoting the digestion and absorption of fat, allowing animals to more fully utilize the nutrients in fish oil, such as Omega-3 fatty acids, to meet their needs for healthy fats and promote hair and skin health.

[0021] (2) Improved palatability: The fish oil emulsion treated with bile salt emulsification can effectively mask the fishy smell of fish oil and reduce its adverse stimulation to the animal's sense of smell and taste, thereby improving the product's palatability. At the same time, the solid particle form of the microcapsule also helps to mask the unpleasant smell and taste of fish oil, making animals more willing to accept and consume it, ensuring the smooth intake of nutrients.

[0022] (3) Enhanced antioxidant properties and stability: In the wall material design of the microcapsule, the inner wall material is made of solidified alginate, which has good oxygen barrier properties and can effectively prevent oxygen from entering the microcapsule, slowing down the oxidation reaction of fish oil and extending the shelf life of the product. The outer wall material is made of nanocrystalline cellulose, which has excellent water barrier properties and can prevent moisture from penetrating into the microcapsule, maintaining a dry environment inside the microcapsule, further reducing the risk of fish oil oxidation. It also helps to maintain the structural integrity and stability of the microcapsule, making it less likely to deform and rupture during storage and transportation.

[0023] (4) Achieve targeted release in the intestine: The structural design of the microcapsules makes them difficult to decompose in the stomach acid environment, allowing them to pass smoothly through the stomach into the intestine. In the alkaline environment of the intestine, the wall material of the microcapsules gradually dissolves, achieving targeted release of fish oil. This release method helps reduce the irritation of fish oil in the stomach and avoids adverse reactions caused by fish oil adhering to the gastric mucosa, such as belching, nausea, bad breath, and vomiting. It also improves the absorption efficiency of fish oil in the intestine, allowing animals to better utilize the nutrients in fish oil and achieve health effects such as beautifying hair and skin.

[0024] In summary, the water-blocking microcapsules provided by the present invention, through a composite solid particle structure design composed of core and wall materials, achieve multiple beneficial effects such as efficient digestion and absorption of fish oil, improved palatability, enhanced antioxidant properties and stability, and targeted intestinal release. These advantages provide an innovative technical solution for the development of animal health products and have broad application prospects and market value. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0026] Figure 1 This is a schematic diagram of the structure of the water-blocking microcapsule in the embodiment of the present application; Figure 2 This is a schematic diagram of the structure and morphology of the fish oil compound hair-beautifying cream in the embodiment of this application; Figure 3 This is a comparison chart of the cat hair sensory experience in Experiment 2 of this application.

[0027] Reference numerals: 100-fish oil compound hair-beautifying cream; 1-water-blocking microcapsule; 11-core material; 12-wall material; 121-inner wall material; 122-outer wall material. DETAILED DESCRIPTION

[0028] The embodiments of the present invention will be described in detail below with reference to the examples, but it will be understood by those skilled in the art that the following examples are merely illustrative of the present invention and should not be construed as limiting the scope of the invention. Where specific conditions are not specified in the examples, the methods were performed according to conventional conditions or the conditions recommended by the manufacturer. Where the manufacturers of the reagents or instruments are not specified, they are all commercially available conventional products.

[0029] refer to Figure 1 In an embodiment of the present application, a water-blocking microcapsule is provided, which is a solid particle composed of a core material and a wall material coated on the outside of the core material; wherein the core material is a fish oil emulsion emulsified with bile salts; the wall materials are an inner wall material and an outer wall material from the inside to the outside; the inner wall material is a wall material composed of solidified alginate; the outer wall material is a water-blocking wall material composed of nanocrystalline cellulose and arranged outside the inner wall material.

[0030] As mentioned above, the core material is the main component inside the microcapsule and is composed of an emulsion formed by emulsifying fish oil with bile salts. An emulsion is a mixture formed by dispersing one liquid in another. It can be formed by emulsifying components such as an oil phase, an aqueous phase, and an emulsifier.

[0031] The bile salts mentioned above may include, but are not limited to, sodium taurocholate and / or sodium glycinecholate. The fish oil may be concentrated fish oil, sourced from, but not limited to, anchovy oil and salmon oil, and its omega-3 content should be no less than 50%. Omega-3 fatty acids primarily include alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Omega-3 fatty acids have anti-inflammatory properties. They alleviate inflammation by regulating key mediators of the inflammatory response, such as prostaglandins and leukotrienes, and can reduce coat problems caused by inflammation. Omega-3s help maintain normal skin cell metabolism and moisture balance, keeping the skin smooth, soft, and elastic, and maintaining skin health.

[0032] It should be noted that while conventional fish oil microcapsules use untreated fish oil as the core material, the fish oil in this application is emulsified with bile salts to form a stable emulsion. Bile salts, as emulsifiers, can reduce the interfacial tension between the oil and water, allowing the fish oil to be evenly dispersed in the aqueous phase as tiny droplets, forming an emulsion.

[0033] The resulting core material structure is a uniformly dispersed oil-water emulsion system with small oil droplet size and uniform distribution, and has higher stability than conventional fish oil products.

[0034] It should be noted that emulsified fish oil is easier for animals to digest and absorb because the emulsifier makes the fat particles smaller, increases the contact area with digestive enzymes, and promotes the digestion of fat; at the same time, the emulsification process can also mask the fishy smell of fish oil and greatly improve the palatability of the product, thus solving the problem of low digestion and absorption rate and poor palatability of unemulsified fish oil, allowing animals to better utilize the nutrients in fish oil, such as Omega-3 fatty acids, to promote hair and skin health.

[0035] The inner wall material is the inner layer of the microcapsule wall material, and is a solid structure formed by a solidification reaction of alginate. Alginate is a natural polysaccharide with good film-forming properties and biocompatibility.

[0036] While traditional microcapsule preparation uses materials such as gelatin and protein as wall materials, this example uses alginate as the inner wall material, which is then cured to form a stable solid structure. The curing process typically involves exposing an alginate solution to a crosslinking agent such as calcium ions, causing crosslinking reactions between alginate molecules to form a three-dimensional network structure.

[0037] The obtained inner wall material structure is a solid membrane with a three-dimensional network structure, which has certain strength and stability and can wrap and protect the core material.

[0038] The wall material of the cured alginate in this embodiment has excellent oxygen barrier properties and biocompatibility, which can effectively prevent oxygen from entering the interior of the microcapsules, slowing down the oxidation reaction of the fish oil and extending the shelf life of the product. At the same time, it has good biocompatibility and will not cause adverse reactions in animals, thereby solving the problem of fish oil being easily oxidized and deteriorated during storage in the microcapsules, and improving the stability of the microcapsules and the shelf life of the product.

[0039] The outer wall material is the outer layer of the microcapsule wall material and is composed of nanocrystalline cellulose, which has excellent water barrier properties. Nanocrystalline cellulose is a nano-scale cellulose material with unique structure and properties.

[0040] In conventional microencapsulation technology, conventional wall materials do not have a dedicated water barrier design, or other materials are used as a water barrier. In this embodiment, nanocrystalline cellulose is used as the outer wall material, taking advantage of its unique nanostructure and excellent water barrier properties to provide an additional protective layer for the microcapsules. The preparation of nanocrystalline cellulose generally involves nano-processing the cellulose to form a nanoscale fiber structure.

[0041] The resulting outer wall material is a water-resistant membrane with a nanofiber structure that effectively blocks water penetration. The nanocrystalline cellulose, with its excellent water-blocking properties, prevents moisture from penetrating the microcapsules, maintaining a dry environment within the microcapsules and further reducing the risk of fish oil oxidation. Its nanostructure also imparts enhanced mechanical properties and stability to the microcapsules, addressing the problem of water absorption in humid environments, which can lead to structural damage and fish oil oxidation. This improves the stability of the microcapsules and enhances the product's storage performance.

[0042] Structurally, microcapsules consist of a core material and a wall material, with the wall material further divided into an inner layer and an outer layer. The core is encapsulated by the inner wall material, which in turn is encapsulated by the outer wall material, forming a core-shell structure. The connection between the core and inner wall materials is achieved through the alginate curing process, which forms a three-dimensional network structure that encapsulates the core. The connection between the inner and outer wall materials is achieved through the coating or compounding of nanocrystalline cellulose, which forms a water-repellent film on the surface of the inner wall material.

[0043] This core-shell structure design gives the microcapsules good protection and release characteristics. The core material is effectively wrapped and protected, allowing it to pass smoothly through the stomach into the intestines, achieving targeted release in the alkaline environment of the intestines, improving the digestion and absorption rate of fish oil while reducing gastric irritation. This solves the problem of fish oil being easily decomposed by digestive enzymes in the stomach and causing irritation to the stomach, and improves the bioavailability and safety of the microcapsules.

[0044] It's important to note that the digestive systems of animals, especially cats and dogs, are sensitive to certain nutrients (such as omega-3 fatty acids in fish oil). These nutrients are easily destroyed or broken down in harsh environments like stomach acid. Encapsulating these nutrients in microcapsules effectively protects them from external environmental influences, improving their stability in the digestive tract and ensuring smooth intestinal absorption and utilization, thereby increasing their bioavailability and fully realizing their coat-beautifying benefits. Furthermore, cats and dogs are very sensitive to the smell and taste of food. Some nutrients in coat-beautifying products, such as fish oil, may have an unpleasant odor, which can affect their appetite. Microencapsulation technology can encapsulate these ingredients, masking their unpleasant odor and making the products more palatable, making them more readily accepted and consumed by animals, ensuring smooth nutrient intake. Furthermore, the digestive tracts of cats and dogs vary significantly, with the stomach being acidic and the intestines being alkaline. Through microencapsulation technology, microcapsules can be designed to release nutrients in specific areas (such as the intestines) according to the environmental characteristics of the animal's digestive tract, achieving precise release and improving the utilization efficiency of nutrients. At the same time, it can reduce irritation and side effects in areas such as the stomach and protect the health of the animal's digestive system.

[0045] In this embodiment, the inner wall material formed by curing alginate exhibits excellent oxygen barrier properties, effectively preventing oxygen from entering the microcapsules. This slows the oxidation of nutrients like fish oil, extends the product's shelf life, and ensures the stability and effectiveness of these nutrients. In the animal digestive tract, the inner wall material provides a degree of resistance to environmental erosion, such as gastric acid, providing initial protection for the nutrients. Furthermore, in the alkaline environment of the intestine, the inner wall material gradually dissolves, allowing for the initial release of nutrients and paving the way for their subsequent release and absorption.

[0046] The outer wall material (nanocrystalline cellulose water-blocking wall material) has a water-blocking and further protective effect. Nanocrystalline cellulose has excellent water-blocking properties, which can prevent moisture from penetrating into the microcapsules, maintain a dry environment inside the microcapsules, further reduce the risk of nutrient oxidation, and improve the stability of the microcapsules and the storage performance of the product. In addition, the nanostructure of nanocrystalline cellulose gives the microcapsules better mechanical properties and integrity, making the microcapsules less likely to deform and rupture during storage, transportation and use, ensuring the quality and safety of the product.

[0047] The inner wall material and the outer wall material together constitute the double protection barrier of the microcapsule. The inner wall material mainly isolates oxygen, and the outer wall material mainly blocks water. The two work together to more comprehensively protect the nutrients, so that they can remain stable under various environmental conditions and improve the overall stability of the product.

[0048] In addition, in the animal's digestive tract, the inner and outer wall materials have different solubility characteristics. The inner wall material dissolves first in the alkaline environment of the intestine, and the outer wall material dissolves later. This synergistic release mechanism can gradually release nutrients in the intestine, prolong the release time, and improve the absorption efficiency of nutrients, ensuring that animals can fully absorb and utilize the nutrients needed for beautiful hair, achieving better hair beautification effects.

[0049] In summary, the use of microcapsule technology and a double-layer design with different wall materials can fully utilize the physiological characteristics of animals such as cats and dogs, improve the efficacy and safety of hair care products, and better meet the health needs of animals.

[0050] The present application provides a method for preparing the water-blocking microcapsules as described in the aforementioned embodiment, comprising: Step S1: emulsifying concentrated fish oil with bile salts to obtain an O / W / O type emulsion core material.

[0051] In this step, the concentrated fish oil is emulsified by bile salts to form an oil / water / oil (O / W / O) emulsion structure, which serves as the core material for the microcapsules. The concentrated fish oil can be mixed with bile salts. The bile salts act as emulsifiers, reducing the interfacial tension between the oil and water, allowing the fish oil to be evenly dispersed in the aqueous phase as tiny droplets, forming a preliminary oil / water (O / W) emulsion. This O / W emulsion is then mixed with an external oil phase (such as concentrated fish oil). Stirring or other methods are used to encapsulate the O / W emulsion, forming an O / W / O emulsion.

[0052] This step produces a stable O / W / O emulsion core material, in which fish oil (which can be concentrated fish oil) is evenly dispersed in the aqueous phase, improving the dispersibility and stability of the fish oil and making it less likely to aggregate and settle during the subsequent microencapsulation process. Bile salt-emulsified concentrated fish oil water-blocking microcapsules contain concentrated fish oil pre-emulsified with bile salts, making the fish oil easier to digest and more palatable. The main components of bile salts are bile salts and bile acids. Bile salts act as emulsifiers, emulsifying large molecular weight fats. Bile acids combine with fatty acids to form water-soluble complexes, thereby promoting fat absorption and metabolism. Bile salts can also disrupt the surface tension of large fat particles, breaking them down into smaller clusters, making them relatively easier to digest and absorb in the stomach and intestines. They also increase the action of pancreatic lipase, promoting fat digestion and absorption.

[0053] Specifically, this can be achieved through emulsification processes such as high-speed stirring and ultrasonic treatment. Specific parameters such as stirring speed and temperature can be optimized according to actual needs.

[0054] Step S2: mixing the O / W / O type emulsion core material with a whey protein aqueous solution and alginate to obtain a whey mixed solution.

[0055] The above steps are to mix the O / W / O type emulsion core material with a whey protein aqueous solution and alginate to form a mixed solution, preparing for subsequent microencapsulation. In this step, whey protein is dissolved in water to form a whey protein aqueous solution, which has good film-forming properties and stability, and alginate is dissolved in water to form an alginate aqueous solution, which has good film-forming properties and biocompatibility; the O / W / O type emulsion core material is uniformly mixed with the whey protein aqueous solution and the alginate aqueous solution to form a whey mixed solution, thereby obtaining a uniformly mixed whey mixed solution, in which the O / W / O type emulsion core material is wrapped by the whey protein and alginate, providing a basis for subsequent microencapsulation.

[0056] The addition of whey protein and alginate increases the viscosity and stability of the mixture, facilitating the subsequent formation of microcapsules. The film-forming properties of whey protein and alginate contribute to the formation of microcapsule wall materials with excellent mechanical properties and biocompatibility.

[0057] Specifically, it can be achieved through mixing means such as stirring, and specific parameters such as stirring speed and time can be optimized according to actual needs.

[0058] Step S3, adding the whey mixture dropwise into the first calcium lactate gluconate solution, and performing a first solidification treatment to obtain a single-layer microcapsule coated with an inner wall material.

[0059] In the above steps, the whey mixture is added dropwise to the calcium lactate gluconate solution. The calcium ions cross-link with the alginate to solidify the alginate, forming the inner wall material of the microcapsules and obtaining single-layer microcapsules. Calcium lactate gluconate is dissolved in water to form a calcium lactate gluconate solution with a concentration of 5% w / v to 10% w / v and a pH of 3 to 5. The whey mixture is then added dropwise to the calcium lactate gluconate solution. During the addition process, the calcium ions cross-link with the alginate to form a calcium alginate gel, which encapsulates the O / W / O emulsion core material to form single-layer microcapsules.

[0060] In this step, single-layer microcapsules coated with an inner wall material (calcium alginate gel) are obtained. The microcapsules have certain strength and stability. The calcium alginate gel has good oxygen barrier properties and biocompatibility, which can effectively protect the fish oil inside the microcapsules and prolong their shelf life. By controlling parameters such as dropping speed and stirring speed, the particle size and uniformity of the microcapsules can be adjusted.

[0061] Specifically, the whey mixture can be added dropwise into the calcium lactobionate solution using a dropping device (such as a dropper, sprayer, etc.), while stirring to facilitate the cross-linking reaction.

[0062] In step S4, the single-layer microcapsules are added to a nanocrystalline cellulose solution, and after a second solidification treatment, double-layer microcapsules are obtained.

[0063] In this step, the single-layer microcapsules are added to a nanocrystalline cellulose solution. The nanocrystalline cellulose forms an outer wall material for the microcapsules, resulting in double-layer microcapsules.

[0064] In this step, nanocrystalline cellulose is dissolved in water to form a nanocrystalline cellulose solution with a mass concentration of 0.3% to 2%. The single-layer microcapsules are added to the nanocrystalline cellulose solution. Under certain conditions (such as temperature, pH, etc.), nanocrystalline cellulose will form a dense water-blocking film on the surface of the microcapsules as an outer wall material, resulting in double-layer microcapsules coated with an outer wall material (nanocrystalline cellulose film). The microcapsules have better water-blocking performance and mechanical properties.

[0065] Nanocrystalline cellulose has excellent water-blocking performance, which can prevent water from penetrating into the microcapsules, further improving the stability of the microcapsules. The nanocrystalline structure of nanocrystalline cellulose gives the microcapsules better mechanical properties, making them less likely to deform and break during storage, transportation, and use.

[0066] Specifically, the single-layer microcapsules can be mixed with the nanocrystalline cellulose solution by stirring, and specific parameters such as stirring speed and time can be optimized according to actual needs.

[0067] In step S5, the double-layer microcapsules are dispersed in a second calcium lactobionate solution, and after a third solidification treatment, sodium chloride solution cleaning treatment, and freeze-drying, the water-blocking microcapsules are obtained.

[0068] In this step, the double-layer microcapsules are further dispersed in a calcium lactate gluconate solution, subjected to a third curing treatment, then washed with a sodium chloride solution, and finally freeze-dried to obtain the final water-blocking microcapsules, that is, calcium lactate gluconate is dissolved in water to form a second calcium lactate gluconate solution with a concentration of 1% w / v~3% w / v and a pH of 1~3; the double-layer microcapsules are dispersed in the second calcium lactate gluconate solution, and a third curing treatment is performed under certain conditions to further enhance the strength and stability of the microcapsule wall material; the microcapsules are washed to remove impurities such as calcium lactate gluconate remaining on the surface, so that the surface of the microcapsules is clean and smooth, and the washed microcapsules are freeze-dried to remove moisture, thereby obtaining dry water-blocking microcapsules with good water-blocking properties, mechanical properties and stability, and are easy to store and use.

[0069] The third curing treatment can further enhance the strength and stability of the microcapsule wall material and improve the durability of the microcapsules. The sodium chloride solution cleaning treatment can remove impurities on the surface of the microcapsules and improve the purity and surface quality of the microcapsules.

[0070] Among them, freeze-drying can effectively remove moisture from microcapsules, maintain the shape and structure of microcapsules, and extend their shelf life.

[0071] Specifically, the double-layer microcapsules can be dispersed into the second calcium lactate gluconate solution by stirring, dropping, spraying, etc., and then solidified; when washing with sodium chloride solution, immersion, spraying, etc. can be used; freeze drying can be carried out under low temperature conditions, and specific parameters such as temperature and time can be optimized according to actual needs.

[0072] Furthermore, the first calcium lactate gluconate solution is a calcium lactate gluconate solution having a concentration of 5% w / v to 10% w / v and a pH of 3 to 5; for example, the concentration may be 5% w / v, 6% w / v, 7% w / v, 8% w / v, 9% w / v, 10% w / v, etc. The pH may be 3, 4, 5, etc.

[0073] Furthermore, the second calcium lactate gluconate solution is a calcium lactate gluconate solution with a concentration of 1% w / v to 3% w / v and a pH of 1 to 3; for example, the concentration may be 1% w / v, 2% w / v, 3% w / v, etc.; the pH may be 3, 4, 5, etc.

[0074] Furthermore, the weight ratio of the O / W / O type emulsion core material, the alginate and the whey protein aqueous solution is (1-2):(4-5):(4-6); for example, the weight ratio of the O / W / O type emulsion core material in the ratio can be 1, 1.5, 2, etc.; the weight ratio of the whey protein aqueous solution in the ratio can be 4, 4.5, 5, etc.; the weight ratio of the whey protein aqueous solution in the ratio can be 4, 5, 6, etc.

[0075] Furthermore, the mass concentration of the whey protein aqueous solution is 15.0 g / L to 40.0 g / L; for example, it can be 15 g / L, 20 g / L, 25 g / L, 30 g / L, 35 g / L, 40 g / L, etc. Further, the mass concentration of the alginate aqueous solution is 15.0 g / L to 40.0 g / L; for example, it can be 15 g / L, 20 g / L, 25 g / L, 30 g / L, 35 g / L, 40 g / L, etc. Further, the treatment time of the first solidification treatment is 20 minutes to 50 minutes; for example, the treatment time can be 20 minutes, 30 minutes, 40 minutes, 50 minutes, etc. Further, the mass concentration of the nanocrystalline cellulose solution is 0.3% to 2%; for example, the mass concentration can be 0.3%, 0.5%, 0.8%, 1%, 1.5%, 2%, etc. Furthermore, the processing time of the second curing treatment is 2 minutes to 10 minutes; for example, the processing time can be 2 minutes, 3 minutes, 5 minutes, 6 minutes, 7 minutes, 10 minutes, etc.

[0076] Furthermore, the sodium chloride solution cleaning treatment is to use a sodium chloride solution with a mass concentration of 0.5% to 1.5% until the pH is neutral. For example, the mass concentration of the sodium chloride solution can be 0.5%, 0.8%, 1.0%, 1.5%, etc.

[0077] As mentioned above, neutral refers to the property of a solution with a pH value close to 7. During the cleaning process of the microcapsules, the microcapsules are rinsed with sodium chloride solution until the pH value of the solution on the surface of the microcapsules reaches a neutral state close to 7. The purpose of this is to remove any acidic or alkaline substances that may remain on the surface of the microcapsules, ensuring that the chemical properties of the microcapsule surface are stable and will not have adverse effects on subsequent use or storage.

[0078] Furthermore, the step of adding the whey mixture dropwise to the first calcium lactate gluconate solution and performing the first solidification treatment to obtain a single-layer microcapsule coated with an inner wall material further comprises: The single-layer microcapsules are washed with a sodium chloride solution having a mass concentration of 0.5% to 1.5% until the pH reaches neutral, thereby obtaining the washed single-layer microcapsules. The mass concentration of the sodium chloride solution can be 0.5%, 1.0%, 1.5%, etc.

[0079] Furthermore, the whey protein aqueous solution is prepared by adding whey protein to water, stirring continuously for 1 to 2 hours, and centrifuging at 3000 to 8000 rpm for 10 to 15 minutes, and obtaining the supernatant as the whey protein aqueous solution. For example, the centrifugal speed can be 3000 rpm, 5000 rpm, 7000 rpm, 8000 rpm, etc. The centrifugation time can be 10 minutes, 12 minutes, 14 minutes, 15 minutes, etc.

[0080] Furthermore, before the concentrated fish oil is emulsified with bile salts to obtain the O / W / O type emulsion core material, the process further includes: separately preparing an inner oil phase mixture, an aqueous phase mixture, and an outer oil phase mixture; Furthermore, the inner oil phase mixture includes the following components in parts by weight: 15 to 20 parts of a first oil component; for example, 15, 16, 17, 19, 20, etc. 1 to 4 parts of a bile salt-containing emulsifier; for example, 1, 2, 3, 4, etc. wherein the first oil component is concentrated fish oil containing no less than 50% Omega-3; Furthermore, the outer oil phase mixture includes the following components in parts by weight: 25-45 parts of a second oil component (e.g., 25, 30, 35, 40, 45, etc.); 1-10 parts of a W / O emulsifier (e.g., 1, 3, 5, 7, 9, 10, etc.). The second oil component is concentrated fish oil containing no less than 50% omega-3. The first and second oil components may contain the same or different amounts.

[0081] Furthermore, the aqueous phase mixture includes the following components in parts by weight: 1-10 parts of an O / W emulsifier (e.g., 1, 3, 5, 7, 9, 10, etc.); 2-5 parts of a co-emulsifier (e.g., 2, 3, 4, 5, etc.); and 25-45 parts of water (e.g., 25, 30, 35, 40, 45, etc.).

[0082] Further, the water phase comprises at least one of glycerol, bovine serum albumin, sodium alginate, peptide, chitosan, collagen. Further, the cholate salt of the bile salt co-emulsifier in the inner oil phase mixture comprises taurine cholate sodium salt and / or glycine cholate sodium salt. Further, the O / W emulsifier in the water phase mixture is a hydrophilic emulsifier; further, the O / W emulsifier in the water phase mixture comprises at least one of Tween 20, Tween 40, Tween 60 and Tween 80; further, the W / O emulsifier in the outer oil phase mixture is a lipophilic emulsifier; further, the W / O emulsifier in the outer oil phase mixture comprises at least one of Span 20, Span 40, Span 60 and Span 80; further, the co-emulsifier comprises n-butanol, ethylene glycol, ethanol, propylene glycol, glycerol and polyglycerol ester. Further, the weight ratio of the inner oil phase mixture, the water phase mixture and the outer oil phase mixture is (1-2):(1-4):(1-2).

[0083] Further, the emulsification treatment of the concentrated fish oil by using the bile salt to obtain the O / W / O emulsion core material comprises: preheating the inner oil phase mixture, the water phase mixture and the outer oil phase mixture respectively; stirring the water phase mixture and adding the inner oil phase mixture, and then ultrasonic treatment to obtain an O / W mixed emulsion; adding the outer oil phase mixture to the O / W mixed emulsion, and then stirring treatment to obtain the O / W / O emulsion core material.

[0084] Further, the preheating temperature is not less than 85℃.

[0085] Further, in the step of stirring the water phase mixture and adding the inner oil phase mixture, and then ultrasonic treatment to obtain an O / W mixed emulsion, the stirring rate is 500r / min.

[0086] Further, in the step of adding the outer oil phase mixture to the O / W mixed emulsion, and then stirring treatment to obtain the O / W / O emulsion core material, the stirring treatment comprises: first stirring at a stirring rate of 650r / min; the stirring time is not less than 30 minutes; then second stirring at a stirring rate of 580r / min; and continuously stirring until cooling to room temperature.

[0087] In a third aspect, the present application provides a fish oil compound hair conditioner, which is a compound conditioner composed of the water-blocking microcapsule as described in the foregoing embodiments, and an oil component, a surfactant, a protein component, an amino acid component, a mineral component, a vitamin mixture, an organic acid component, a thickening agent, a sugar substance and water.

[0088] Further, the oil component includes at least one of fish oil, seal oil, Antarctic krill oil, and seaweed oil. The oil component can be other OMEGA 3 oil components, which can include but are not limited to fish oil (for example, deep sea fish oil), seal oil, Antarctic krill oil, and seaweed oil. The above-mentioned oil substances of EPA and DHA can also be additionally added. Omega-3 fatty acids mainly include alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), etc. Omega-3 fatty acids have anti-inflammatory effects. It can alleviate the inflammatory response by regulating key mediators such as prostaglandins and leukotrienes, and reduce the hair problems caused by inflammation. Omega-3 can help maintain normal metabolism and water balance of skin cells, keep the skin smooth, soft and elastic, and maintain skin health.

[0089] Further, the surfactant includes at least one of egg yolk powder, soy lecithin, and egg yolk lecithin. The source of the surfactant can include but is not limited to egg yolk powder, soy lecithin, and egg yolk lecithin, and the existence form can include but is not limited to liquid, particles, powder, and other lecithin-containing surfactants. Lecithin not only has the function of a surfactant, but also has the function of beautifying and protecting the skin. Lecithin contains inositol phospholipids, which can promote hair growth and prevent hair loss. In addition, lecithin can supplement choline substances, which can work together with other B vitamins to meet the growth needs of hair. Studies have shown that feeding lecithin can make the hair of pets more shiny, reduce the damage rate of hair, improve the quality of hair, and thus improve the skin condition. Lecithin also has antioxidant capacity, can scavenge oxygen free radicals, protect cells, and further improve the quality of hair. Lecithin is also beneficial to the skin. It can decompose fat and promote the absorption of unsaturated fatty acids, which are very beneficial to the skin and hair of pets.

[0090] Further, the protein component includes at least one of bone glue, milk powder, yeast extract, chicken hydrolysate, and pig plasma protein; and the protein component can be one or more selected from the group consisting of bone glue, milk powder, yeast extract, chicken hydrolysate, and pig plasma protein powder.

[0091] Protein is a very important nutrient, and 30% of the daily intake of protein by dogs is used to maintain the metabolism of the skin and hair. The hair of some breeds does not fall off regularly and is in a state of continuous growth. The main protein that constitutes the hair is keratin, which is composed of sulfur-containing amino acids. Therefore, these sulfur-containing amino acids play a very important role in maintaining the health of the hair. By providing high-quality protein food, the quality of the hair can be effectively improved, and it is also a high-quality natural flavor attractant ingredient.

[0092] Furthermore, the mineral components include copper and / or zinc; further, the mineral components exist in the form of amino acid complexes and / or oxides; further, the mineral components are copper and zinc, wherein the weight ratio of copper to zinc is (0.01-1.5):(0.01-3.5); The mineral components mentioned above may include, but are not limited to, copper and zinc. These minerals exist as amino acid complexes (chelates) or oxides. Trace elements such as copper and zinc are essential nutrients for animal life and growth and have a wide range of biological functions. For example, copper and zinc act as essential components or activators of many important enzymes in animals, participating in and regulating a range of biochemical reactions and metabolic processes. Insufficient intake of trace elements such as copper and zinc can lead to metabolic disorders, resulting in symptoms such as growth stunting, skeletal hypoplasia, incomplete skin keratinization, poor coat growth, and decreased hematopoietic, reproductive, and immune function. Zinc is a component or activator of many enzymes and is involved in protein synthesis, crucial for growth and development, tissue regeneration, and skin health. Zinc deficiency in pets can lead to skin and coat damage, even peeling. Incomplete keratinization is a typical symptom of zinc deficiency in many animals. Copper supplementation can increase tyrosinase activity, thereby promoting the production and deposition of melanin in the coat, deepening coat color.

[0093] Furthermore, the amino acid component includes L-cysteine ​​and / or L-tyrosine; further, the amino acid component includes L-cysteine ​​and L-tyrosine; wherein the weight ratio of L-cysteine ​​to L-tyrosine is (0.05-5):(0.05-5); The amino acid components mentioned above may include, but are not limited to, L-cysteine ​​and L-tyrosine. Amino acids, as the building blocks of protein, are also involved in coat metabolism. Cystine, among them, can be converted into cysteine ​​in pets, a major component of hair. Therefore, foods rich in cystine can effectively promote coat regeneration. Melanin deposition in pets is related to tyrosinase activity. The higher the tyrosinase activity, the more melanin is produced. Supplementing with tyrosine and copper can increase tyrosinase activity in the body, thereby promoting the production and deposition of melanin in the coat.

[0094] Furthermore, the vitamin mixture includes at least one of vitamin B1, vitamin B2, vitamin B12 and vitamin E; further, the vitamin mixture includes vitamin B1, vitamin B2, vitamin B12 and vitamin E; wherein the weight ratio of vitamin B1, vitamin B2, vitamin B12 and vitamin E is (0.01-1):(0.05-1):(0.05-1):(0.05-1); As mentioned above, a lack of vitamin B2 in pets can lead to hair loss, epidermal atrophy, and dermatitis; a lack of niacin can cause a matted coat; a lack of biotin can manifest as dermatitis, hair loss, and hair discoloration; and a lack of vitamin B12 can cause a rough coat and dermatitis. Because B vitamins work synergistically, supplementing with multiple B vitamins simultaneously is more beneficial to maintaining a healthy coat.

[0095] Furthermore, the organic acid includes citric acid and / or lactic acid; further, the carbohydrate substance includes monosaccharide and / or disaccharide; further, the thickener includes at least one of gelatin, sodium carboxymethyl cellulose, xanthan gum, pectin, gellan gum and locust bean gum.

[0096] Furthermore, the fish oil compound hair-beautifying cream includes the following components in parts by weight: 10-40 water-blocking microcapsules; for example, 10, 20, 30, 40, etc.; 10-40 oil and fat components; for example, 10, 20, 30, 40, etc.; 0.2-7 surfactants; for example, 0.2, 0.5, 0.8, 1, 3, 5, 7, etc.; 15-20 protein components; for example, 15, 18, 19, 20, etc.; 0.5-5 amino acid components; for example, 0.5, 0.8, 1, 2, 3, 4, 5, etc.; 0.1-0.8 mineral components; for example, 0.1, 0.2, 0.4, 0.6, 0.8, etc.; 0.1-1 vitamin mixture; for example, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0, etc. The organic acid component may be 1-10, for example, 1, 3, 5, 7, 9, 10, etc. The thickener may be 1-10, for example, 1, 3, 5, 7, 9, 10, etc. The carbohydrate may be 5-20, for example, 5, 8, 10, 12, 15, 17, 20, etc. The water may be 5-55, for example, 5, 10, 15, 25, 35, 45, 55, etc.

[0097] The present application provides a method for preparing the fish oil compound hair-beautifying ointment as described in any one of the aforementioned embodiments, comprising: Step S100, mixing water and a carrier substance to prepare a carrier solution; step S200, adding water, a protein component, an amino acid component, a vitamin mixture, an organic acid component, and a carbohydrate substance to the carrier solution and stirring and mixing to obtain a nutrient mixture; step S300, homogenizing and stirring the oil component and the surfactant and heating them to 65°C~70°C to obtain a homogenous mixed oil; step S400, adding a thickener to the nutrient mixture and performing a homogenous emulsification treatment, then adding the homogenous mixed oil and performing a homogenous emulsification treatment to obtain an emulsified mixture; step S500, heating the emulsified mixture to 70°C during stirring, adding water-blocking microcapsules, continuing stirring and heating to boiling; step S600, cooling to room temperature to obtain the fish oil composite hair-beautifying cream.

[0098] The present invention is further described below by way of specific examples. However, it should be understood that these examples are merely provided for more detailed description and are not to be construed as limiting the present invention in any form.

[0099] Table 1. Comparison of core material parameters in Examples and Comparative Examples

[0100] In Table 1, “例” represents an embodiment, such as “例 1” refers to Example 1; “比较” represents a comparative example, such as “对1” refers to Comparative Example 1. The same shall apply hereinafter.

[0101] Table 2. Comparison of wall material parameters in Examples and Comparative Examples

[0102] Table 3. Comparison of key process parameters in Examples and Comparative Examples

[0103] Table 4, Comparison of formula parameters of Meimao cream in Examples and Comparative Examples

[0104] Example 1 In this embodiment, a fish oil compound hair-beautifying cream was prepared.

[0105] Experimental methods: 1. Preparation method of double-layer coated water-blocking microcapsules (1) Preparation of O / W / O core material: 1) Components: A. The inner oil phase mixture includes the following components in parts by weight: 20 parts of concentrated fish oil containing 50% Omega 3, purchased from Hunan Wanquan refined fish oil CW30; 4 parts of sodium taurocholate.

[0106] B. The aqueous phase mixture includes the following components in parts by weight: 205 parts of Tween, a hydrophilic emulsifier, as an O / W emulsifier; 5 parts of glycerol as a co-emulsifier; and 40 parts of water.

[0107] C. The outer oil phase mixture includes the following components in parts by weight: 2025 parts of concentrated fish oil containing 50% Omega 3, purchased from Hunan Wanquan Refined Fish Oil CW30; 801 parts of hydrophilic emulsifier Span as the W / O emulsifier.

[0108] 2) Process: A. Preheat the inner oil phase mixture, aqueous phase mixture, and outer oil phase mixture separately at 90°C; B. Stir the aqueous phase mixture and add the inner oil phase mixture. After ultrasonic treatment, stir at a rate of 500 r / min to obtain an O / W mixed emulsion; C. Add the outer oil phase mixture to the O / W mixed emulsion and perform a first stirring at a rate of 650 r / min for 40 minutes, followed by a second stirring at a rate of 580 r / min. Stirring is continued until cooled to room temperature. After stirring, the O / W / O type emulsion core material is obtained. (2) Preparation of wall material: Prepare a 40 g / L whey protein aqueous solution, stir continuously for 2 h, centrifuge at 6000 rpm for 10 min, and retain the supernatant for later use. Prepare a 40 g / L sodium alginate aqueous solution, heat and stir until completely dissolved, cool to room temperature, and set aside; first mix the O / W / O type emulsion core material prepared in step (1) with the sodium alginate aqueous solution, and then add the whey protein aqueous solution (the weight ratio of the O / W / O type emulsion core material, sodium alginate, and whey protein is 1.5:4:4) during slow stirring. Stir at 200 rpm for 20 min to obtain mixed solution A.

[0109] (3) Ca 2+ Primary curing: Prepare a 10% (w / v) calcium gluconate aqueous solution, adjust its pH to 4.0 with hydrochloric acid, sterilize in an autoclave, use a 1 mL 0.3 mm pore needle to draw the mixed solution of step (2), and add it dropwise to the above calcium gluconate aqueous solution (the volume ratio of the mixed solution to the calcium gluconate aqueous solution is 1:1, and the weight ratio of calcium gluconate to alginate is 0.3:5). The curing time is 30 minutes, the stirring speed is 400 rpm, and the dropping speed is 2 to 3 drops / s. Then, collect the microcapsules by centrifugation, and then wash them with a 0.9% mass concentration NaCl aqueous solution until they are neutral and set aside.

[0110] (4) Nanocrystalline cellulose coating: Add the microcapsules to a sterilized nanocrystalline cellulose solution with a mass concentration of 1.5% (the amount of nanocrystalline cellulose used is 10g of nanocrystalline cellulose for 1g of sodium alginate), stir and solidify for 2 minutes to obtain a mixed solution B; Step (5) Ca2+ secondary solidification: the mixed solution B was redispersed into a 3% (w / v) calcium gluconate aqueous solution (the pH of the calcium gluconate solution was adjusted to 3.0 with saline) (the volume ratio of the mixed solution B to the calcium gluconate aqueous solution was 1:3, and the weight ratio of calcium gluconate to alginate was 0.05:5), solidified for 5 minutes at a stirring speed of 400 rpm, and the microcapsules were collected and then clarified with a 0.9% NaCl aqueous solution to neutrality, freeze-dried for 3 hours, and stored for later use to obtain double-layer coated water-blocking microcapsules.

[0111] 2. Preparation of fish oil compound hair-beautifying ointment: (1) Components: double-layer coated water-blocking microcapsules 10%; oil component 10% (krill oil 4%, Schizochytrium oil 3%, concentrated fish oil 50% Omega 34%); surfactant 1.5% (egg yolk lecithin 1.5%); protein component 14% (bone gelatin 5%, milk powder 1%, yeast extract 1%, chicken hydrolyzate 1% and porcine plasma protein 6%); amino acid component 0.5% (L-cysteine ​​0.25% and L-tyrosine 0.25%); mineral component 0.3% ( Methionine chelated copper 0.03%, methionine chelated zinc 0.03%, methionine chelated iron 0.03%, calcium iodate 0.01%, calcium hydrogen phosphate 0.2%); vitamin mixture 0.2% (vitamin B1 0.05%, vitamin B2 0.05%, vitamin B12 0.05% and vitamin E 0.05%); organic acid component 0.3% (lactic acid 0.3%); thickener 2.7% (gelatin 2.7%); carbohydrate 15% (sucrose 15%); water 46.5%.

[0112] (2) Process: Add water, protein components, amino acid components, vitamin mixture, organic acid components, and sugar substances to the carrier solution and stir and mix to obtain a nutrient mixture; stir and homogenize the oil component and the surfactant and heat them to 65°C~70°C to obtain a homogenous mixed oil; add a thickener to the nutrient mixture and perform a homogenous emulsification treatment, then add the homogenous mixed oil and perform a homogenous emulsification treatment to obtain an emulsified mixture; heat the emulsified mixture to 70°C during stirring, add water-blocking microcapsules, continue stirring and heat to boil; cool to room temperature to obtain the fish oil composite hair-beautifying cream.

[0113] Example 2~Example 3, Comparative Example 1~Comparative Example 4: A fish oil compound hair-beautifying cream (or product) was prepared respectively. The preparation method was basically the same as that in Example 1, with reference to the components, amounts and related parameters in Table 1.

[0114] Comparative Example 5 In this comparative example, a fish oil-based hair beautifying product was prepared, wherein the fish oil-based hair beautifying product was directly added without coating and without a core material.

[0115] Preparation method: refer to the method in Example 1. Among the core material parameters, the inner oil phase, water phase, outer oil phase, and emulsification process are not involved; there are no wall material parameters and no curing process.

[0116] Water-blocking microcapsules: 0%; Oil components: oil components 20% (krill oil 4%, Schizochytrium oil 3%, concentrated fish oil 50% Omega3 13%); Surfactant: egg yolk lecithin 1.5%; Protein: protein component 14% (bone gelatin 5%, milk powder 1%, yeast extract 1%, chicken hydrolyzate 1% and porcine plasma protein 6%); Thickener: gelatin 2.7%; Sugar: sucrose 15%.

[0117] 1. Prepare a carrier solution: Mix water and a carrier substance to prepare a carrier solution. 2. Prepare a nutrient mixture: Add water, a protein component, an amino acid component, a vitamin mixture, an organic acid component, and a carbohydrate substance to the carrier solution and stir and mix to obtain a nutrient mixture. 3. Prepare a homogeneous mixed oil: Homogenize and stir the oil component and the surfactant and heat them to 65°C~70°C to obtain a homogeneous mixed oil. 4. Prepare an emulsified mixture: Add a thickener to the nutrient mixture and perform a homogenous emulsification treatment, then add the homogenous mixed oil and perform a homogenous emulsification treatment to obtain an emulsified mixture. 5. Prepare a hair-beautifying cream: Heat the emulsified mixture to 70°C during stirring. Since there are no water-blocking microcapsules, no water-blocking microcapsules are added. Continue stirring and heat to a boil. 6. Cool to room temperature: Cool to room temperature to obtain the fish oil hair-beautifying cream.

[0118] Test experiment 1: Palatability test (double-blind experiment on dogs and cats) 1. Experimental Animals: 20 healthy adult cats were recruited and acclimated to a basic diet for one week prior to the experiment. Ethical Requirements: Animal experiment ethics review was completed to ensure no force-feeding was performed.

[0119] 2. Test method: Double basin preference test (n=10 per group): At the same time, a control group (comparative example 5, unencapsulated fish oil) and an experimental group (microencapsulated fish oil in example 1) were provided (with identical appearance and smell, only the oil form was different), and the ratio of food intake within 30 minutes (food intake of the experimental group / total food intake) was recorded.

[0120] Behavioral indicators: number of first choices, licking duration, rejection response (such as shaking head, retreating).

[0121] Single bowl intake test (3 consecutive days): Provide experimental or control groups separately, and record the daily intake (g / kg body weight) and residual amount.

[0122] 3. Data recording: Calculation method: palatability score = (experimental group food intake - control group food intake) / (experimental group + control group food intake) x 100%.

[0123] Behavioral video analysis: Quantify the licking frequency and duration using AI algorithm (DeepLabCut).

[0124] 3. Test results: Table 5, test results

[0125] The feeding amount of Example 1 was significantly higher than that of Comparative Example 5 (58.7% vs 43.2%), and the licking time increased by 32%, indicating that microencapsulation effectively masked the fishy smell of fish oil. Behavioral observation showed that the first selection frequency of the experimental group accounted for 68% (32% in the control group), and the rejection reaction decreased by 55% (the number of head shaking decreased from 15 times / test to 7 times).

[0126] Test experiment 2: 1. Analysis of fur coat efficacy (1) Experimental method: The test period was 45 days. During the test period, 16 healthy adult cats with a body weight of 3.40±0.68 kg were selected, and they were randomly divided into 2 groups. One group (test group, Example 1) was added with 8 cm of Meimaocao in the basic feed, and the other group (control group) was only fed with the basic feed. There were 8 replicates in each group, and each replicate (each cat) was single-caged in a pet cat cage (1.6 m x 0.7 m x 0.7 m). Necessary immunization and deworming treatment was carried out before the test. The cage was cleaned and disinfected every day to keep the pet cage clean. During the test period, the feeding status, mental state, and adverse reactions such as aversion, diarrhea, and vomiting of all pets were observed.

[0127] Fur index determination: On the 45th day of the test, the fur sensory evaluation (hair brightness, hair softness, skin elasticity), fur length, fur fineness, fur color difference (L*, a*, b*), fur elasticity recovery rate, fur elongation rate, hair loss weight, skin moisture, skin oil, skin pH, and other indicators of the pet cats were determined.

[0128] Hair loss test: Select the fur of a certain area on the left and right sides of the middle back, and use the comb to comb the area 10 times in order, collect the fallen fur, and weigh it on a one-hundredth precision balance to record the effect of Meimaocao on the fur sensory index of pet cats.

[0129] Table 6, effect of Meimaocao on fur sensory index of pet cats

[0130] As shown in Table 6, there was no significant difference in the sensory indexes of cat fur between the experimental group and the control group ( P >0.05). Figure 3 It can be seen that the hair of the cats in the test group feels more shiny and smooth, while the hair of the cats in the control group has a duller sheen compared to the test group.

[0131] 2. Effects of hair beautification cream on the physical and chemical properties of cat fur Table 7. Effects of hair beautifying cream on the physical and chemical properties of pet cats' coat

[0132] Table 8. Effects of hair beautifying cream on the physical and chemical properties of pet cat skin

[0133] According to the data analysis results in Tables 7 and 8, the coat brightness (L*), skin oil content, and skin pH of the cats in the experimental group were significantly higher than those in the control group (P < 0.05), while no significant differences were observed in other coat indicators between the two groups (P > 0.05). This indicates that the hair beautifying cream can effectively enhance the brightness of pet cats' coats, while also increasing the skin oil content and improving the skin pH, helping to maintain the skin barrier function and prevent moisture loss.

[0134] Test experiment 3: Water and oil balance analysis Experimental methods: 1. Water content determination: The total water content of the ointment was determined using the Karl Fischer method (n=5 per group), and the difference between the experimental group (Example 3) and the control group (Example 1) was compared.

[0135] 2. Oil phase distribution uniformity: Cryo-SEM observation: Observe the dispersion state of the microcapsules in the paste (whether they are aggregated) after cryosectioning.

[0136] Laser confocal imaging: The oil phase was stained with Nile red, and the proportion of oil phase accumulation area was calculated (ImageJ software analysis).

[0137] Experimental results: Table 9. Water-oil balance investigation results

[0138] Table 10. Microscopic mechanism evidence

[0139] Analysis: In test experiment 3, a comparative analysis of the oil phase aggregation area and centrifugal stratification rate between the microencapsulated and controlled groups yielded the following conclusions: The oil phase aggregation area and centrifugal stratification rate of the microencapsulated group were significantly reduced. Scanning electron microscopy (SEM) observations showed that the microcapsules were evenly dispersed in the paste, while the oil droplets in the control group aggregated into clusters. This fully demonstrates that microencapsulation can significantly improve the uniformity of oil phase distribution and significantly reduce the centrifugal stratification rate. Further analysis showed that there is a strong correlation between the centrifugal stratification rate and the oil phase aggregation area. This relationship can be described by the formula "stratification rate = 0.32 × aggregation area + 0.11", which has a high goodness of fit (R 2 =0.94), and the statistical significance level was extremely low (P<0.001). Based on this relationship, the safety threshold was set as follows: when the aggregate area is less than 10%, the delamination rate should be less than 3.5%, and the experimental embodiment groups all met this standard. Conversely, when the aggregate area exceeds 30%, the delamination rate will exceed 10%, and the experimental results of the control group (baseline group) exceeded this safety threshold.

[0140] Test experiment 4: Stability verification Experimental methods: 1. Physical stability: Centrifugal stratification test: Take 10g of paste and centrifuge at 3000r / min for 15 minutes, and calculate the volume percentage of the precipitate.

[0141] Rheological properties: Determination of thixotropic loop area (shear rate 0.1→100→0.1s⁻¹) to evaluate structural recovery.

[0142] 2. Chemical stability: Oxidative stability: Accelerated oxidation test at 60°C, with peroxide value (POV) and thiobarbituric acid stability (TBARS) measured weekly.

[0143] pH monitoring: The pH value of the ointment was measured weekly to observe the effect of microencapsulation on the acid-base balance.

[0144] Table 11. Physical stability results (centrifugal delamination rate)

[0145] Table 12. Physical stability results (rheological properties: thixotropic ring area)

[0146] Table 13. Chemical stability (oxidation index change trend - 60℃ accelerated)

[0147] Table 14. Chemical stability: Oxidation and rancidity risks effectively controlled

[0148] Table 15. pH stability monitoring

[0149] Test experiment 5: Double-layer wall material effect comparison experiment In this test experiment, the contribution of the outer layer of nanocrystalline cellulose to the water barrier performance was investigated.

[0150] 1. Experimental methods: (1) Experimental Groups: Control Group Comparative Example 1: Single-layer microcapsules. Experimental Group Example 1: Double-layer microcapsules. Blank Group Comparative Example 5: Uncoated fish oil ointment (for baseline comparison).

[0151] (2) Experimental steps: Sample preparation: Prepare single-layer and double-layer microcapsules according to the patented method and prepare the corresponding ointments. Ensure that the fish oil content is consistent (e.g., 10% w / w).

[0152] (3) Moisture migration test (LF-NMR): The paste samples were placed in a 40°C / 75% RH environment for accelerated aging. Samples were taken at 0, 1, 2, and 3 months, and the transverse relaxation time T2 (ms) was measured using a low-field nuclear magnetic resonance spectrometer. Data recording: n = 3 per group, and the mean ± standard deviation of T2 was calculated.

[0153] (4) Determination of centrifugal stratification rate: Take 10g of paste and centrifuge at 3000r / min for 15 minutes. Calculate the volume percentage of the precipitate. Data recording: n = 5 per group, and take the average value.

[0154] 2. Experimental results: Table 16. Water migration (LF-NMR transverse relaxation time T2, unit: ms)

[0155] Table 17. Distraction and stratification rate results

[0156] Test experiment 6: Bile salt emulsification verification experiment In this test experiment, the bile salt emulsification treatment was investigated to improve the digestion and absorption rate of fish oil.

[0157] 1. Test method: (1) Experimental groups: Control group: microencapsulated non-emulsified fish oil comparative example 2. Experimental group: microencapsulated fish oil (emulsified with bile salts) example 1. Blank group: ointment base without fish oil.

[0158] (2) Experimental steps: 1) In vitro digestion experiment: gastric phase: mix the paste sample with simulated gastric juice (containing pepsin, pH 2.0), shake at 37°C for 2 hours. Intestinal phase: add simulated intestinal juice (containing pancreatic enzyme, bile salt, pH 7.0), continue to shake for 4 hours. Take samples regularly and detect free fatty acid (FFA) release rate (titration method or colorimetric method).

[0159] 2) Data collection: FFA release rate = (amount of released FFA / total fatty acid amount) x 100%. Detection time points: 0, 2, 4, 6 hours.

[0160] 2, test results: Table 18, in vitro digestion experiment (free fatty acid FFA release rate, unit: %)

[0161] Analysis: In test experiment 6, the digestion and absorption efficiency of microencapsulated fish oil (treated with bile salt emulsion) and microencapsulated non-emulsified fish oil were compared by in vitro simulated digestion experiment. The experimental results showed that at 6 hours, the free fatty acid (FFA) release rate of the experimental group reached 68.4%, which was significantly higher than that of the control group (35.2%) (P<0.001), which indicated that bile salt emulsion treatment could significantly improve the digestion and absorption efficiency of fish oil. Especially in the intestinal phase (4~6 hours), the FFA release rate of the experimental group increased by 94.3%, which was significantly higher than that of the control group, further confirming the significant advantage of bile salt emulsion treatment in promoting the digestion and absorption of fish oil.

[0162] Finally, it should be pointed out that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A water-blocking microcapsule, characterized in that: The water-blocking microcapsules are solid particles composed of a core material and a wall material covering the core material; Wherein, the core material is fish oil emulsion emulsified with bile salts; The wall materials are inner wall material and outer wall material from inside to outside; the inner wall material is a wall material made of solidified alginate; the outer wall material is a water-blocking wall material made of nanocrystalline cellulose and arranged outside the inner wall material.

2. A method for preparing the water-blocking microcapsules as claimed in claim 1, characterized in that: include: Concentrated fish oil is emulsified with bile salts to obtain an O / W / O emulsion core material; mixing the O / W / O type emulsion core material with a whey protein aqueous solution and alginate to obtain a whey mixed solution; adding the whey mixture dropwise to the first calcium lactate gluconate solution, and performing a first solidification treatment to obtain a single-layer microcapsule coated with an inner wall material; adding the single-layer microcapsules to a nanocrystalline cellulose solution, and performing a second solidification treatment to obtain double-layer microcapsules; Dispersing the double-layer microcapsules into a second calcium lactate gluconate solution, and performing a third solidification treatment, a sodium chloride solution washing treatment, and freeze-drying to obtain the water-blocking microcapsules; Preferably, the first calcium lactate gluconate solution is a calcium lactate gluconate solution with a concentration of 5% w / v to 10% w / v and a pH of 3 to 5; Preferably, the second calcium lactate gluconate solution is a calcium lactate gluconate solution with a concentration of 1% w / v to 3% w / v and a pH of 1 to 3; Preferably, the weight ratio of the O / W / O type emulsion core material, the alginate and the whey protein aqueous solution is (1-2):(4-5):(4-6); Preferably, the mass concentration of the whey protein aqueous solution is 15.0 g / L to 40.0 g / L; Preferably, the mass concentration of the alginate aqueous solution is 15.0 g / L to 40.0 g / L; Preferably, the treatment time of the first curing treatment is 20 minutes to 50 minutes; Preferably, the mass concentration of the nanocrystalline cellulose solution is 0.3% to 2%; Preferably, the second curing treatment takes 2 to 10 minutes. Preferably, the sodium chloride solution cleaning treatment is cleaning with a sodium chloride solution having a mass concentration of 0.5% to 1.5% until the pH is neutral.

3. The method for preparing water-blocking microcapsules according to claim 2, wherein: The step of adding the whey mixture dropwise to the first calcium lactate gluconate solution and performing the first solidification treatment to obtain a single-layer microcapsule coated with an inner wall material further comprises: The single-layer microcapsules are washed with a sodium chloride solution having a mass concentration of 0.5% to 1.5% until the pH value reaches neutral, thereby obtaining the washed single-layer microcapsules.

4. The method for preparing water-blocking microcapsules according to claim 2, wherein: The preparation method of the whey protein aqueous solution is: Add whey protein to water and continue stirring for 1 to 2 hours; centrifuge at 3000 rpm to 8000 rpm for 10 to 15 minutes, and take the supernatant as the whey protein aqueous solution.

5. The method for preparing water-blocking microcapsules according to claim 2, wherein: Before the concentrated fish oil is emulsified by bile salts to obtain the O / W / O type emulsion core material, the method further includes: preparing an inner oil phase mixture, a water phase mixture and an outer oil phase mixture respectively; Preferably, the inner oil phase mixture includes the following components in parts by weight: The first oil component is 15-20; Contains 1 to 4 bile salt co-emulsifiers; Wherein, the first oil component is concentrated fish oil containing no less than 50% of Omega 3; Preferably, the outer oil phase mixture includes the following components in parts by weight: The second oil component is 25~45; W / O emulsifier 1~10; Wherein, the second oil component is concentrated fish oil containing not less than 50% of Omega 3; Preferably, the aqueous phase mixture comprises the following components in parts by weight: O / W emulsifier 1~10; Co-emulsifier 2~5; Water 25~45; Preferably, the bile salt containing bile salt emulsifier in the internal oil phase mixture includes taurocholate sodium salt and / or glycinecholate sodium salt; Preferably, the O / W emulsifier in the aqueous phase mixture is a hydrophilic emulsifier; Preferably, the O / W emulsifier in the aqueous phase mixture comprises at least one of Tween 20, Tween 40, Tween 60 and Tween 80; Preferably, the W / O emulsifier in the external oil phase mixture is a lipophilic emulsifier; Preferably, the W / O emulsifier in the external oil phase mixture includes at least one of Span 20, Span 40, Span 60 and Span 80; Preferably, the co-emulsifier includes n-butanol, ethylene glycol, ethanol, propylene glycol, glycerol and polyglycerol esters; Preferably, the weight ratio of the inner oil phase mixture, the water phase mixture and the outer oil phase mixture is (1-2):(1-4):(1-2).

6. The method for preparing water-blocking microcapsules according to claim 5, characterized in that: The concentrated fish oil is emulsified using bile salts to obtain an O / W / O type emulsion core material, comprising: Preheating the inner oil phase mixture, the water phase mixture, and the outer oil phase mixture respectively; Stirring the aqueous phase mixture, adding the inner oil phase mixture, and ultrasonically treating to obtain an O / W mixed emulsion; adding the outer oil phase mixture to the O / W mixed emulsion, and stirring to obtain the O / W / O type emulsion core material; Preferably, the temperature of the preheating treatment is not less than 85°C; Preferably, in the step of stirring the aqueous phase mixture and adding the inner oil phase mixture, after ultrasonic treatment, to obtain an O / W mixed emulsion, the stirring rate is 500 r / min.

7. The method for preparing water-blocking microcapsules according to claim 6, wherein: The step comprises adding the external oil phase mixture to the O / W mixed emulsion, and stirring to obtain the O / W / O type emulsion core material. The stirring process comprises: first performing a first stirring at a stirring rate of 650 r / min; stirring for no less than 30 minutes; then performing a second stirring at a stirring rate of 580 r / min; and continuing stirring until cooling to room temperature.

8. A fish oil compound hair-beautifying ointment, characterized in that: The fish oil composite hair-beautifying ointment is a composite ointment composed of the water-blocking microcapsules according to claim 1, and a fat component, a surfactant, a protein component, an amino acid component, a mineral component, a vitamin mixture, an organic acid component, a thickener, a sugar substance and water. Preferably, the oil component comprises at least one of fish oil, seal oil, Antarctic krill oil and algae oil; Preferably, the surfactant comprises at least one of egg yolk powder, soy lecithin and egg yolk lecithin; Preferably, the protein component comprises at least one of bone gelatin, milk powder, yeast extract, chicken hydrolysate and porcine plasma protein; Preferably, the mineral component comprises copper and / or zinc; Preferably, the mineral component exists in the form of amino acid complexes and / or oxides; Preferably, the mineral components are copper and zinc, wherein the weight ratio of copper to zinc is (0.01-1.5):(0.01-3.5); Preferably, the amino acid component includes L-cysteine ​​and / or L-tyrosine; Preferably, the amino acid component comprises L-cysteine ​​and L-tyrosine; wherein the weight ratio of L-cysteine ​​to L-tyrosine is (0.05-5):(0.05-5); Preferably, the vitamin mixture comprises at least one of vitamin B1, vitamin B2, vitamin B12 and vitamin E; Preferably, the vitamin mixture comprises vitamin B1, vitamin B2, vitamin B12 and vitamin E; wherein the weight ratio of vitamin B1, vitamin B2, vitamin B12 and vitamin E is (0.01-1):(0.05-1):(0.05-1):(0.05-1); Preferably, the organic acid comprises citric acid and / or lactic acid; Preferably, the carbohydrate substance includes monosaccharides and / or disaccharides; Preferably, the thickener comprises at least one of gelatin, sodium carboxymethyl cellulose, xanthan gum, pectin, gellan gum and locust bean gum.

9. The fish oil compound hair-beautifying ointment according to claim 8, wherein The fish oil compound hair-beautifying ointment includes the following components in parts by weight: Water-blocking microcapsules 10~40; Oil and fat components 10~40; Surfactant 0.2~7; Protein components 15-20; Amino acid composition 0.5~5; Mineral component 0.1~0.8; Vitamin mixture 0.1~1; Organic acid components 1 to 10; Thickener 1~10; Sugars 5~20; Water 5~55.

10. A method for preparing the fish oil compound hair-beautifying ointment according to any one of claims 8 to 9, characterized in that: include: Mixing water and a carrier substance to prepare a carrier solution; Adding water, protein components, amino acid components, vitamin mixture, organic acid components, and carbohydrate substances to the carrier solution and stirring and mixing to obtain a nutrient mixture; The oil components and surfactants are homogenized, stirred, and heated to 65°C to 70°C to obtain a homogeneous mixed oil; adding a thickener to the nutrient mixture and performing a homogenization and emulsification treatment, then adding the homogenized mixed oil and performing a homogenization and emulsification treatment to obtain an emulsified mixture; The emulsified mixture was heated to 70° C. during stirring, water-blocking microcapsules were added, and stirring was continued while heating to boiling; Cooling to room temperature obtains the fish oil compound hair-beautifying cream.

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