SRAP molecular marking method for genetic diversity analysis of tetracera asiatica

A technique of genetic diversity and molecular markers, which is applied in the field of SRAP molecular markers for the analysis of the genetic diversity of the vine, can solve problems such as the study of the genetic diversity of the vine, and achieve clear bands, high polymorphism, and high The effect of polymorphism

Pending Publication Date: 2020-07-31
GUANGXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

In view of these, SRAP molecular marker technology has been applied in the study of genetic differences, kinship, and genetic map construction of medicinal plants such as Dendrobium, Atractylodes macrocephala, ginseng, and Dipsacus. Study on Genetic Diversity of Tin Leaf Vine

Method used

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  • SRAP molecular marking method for genetic diversity analysis of tetracera asiatica
  • SRAP molecular marking method for genetic diversity analysis of tetracera asiatica
  • SRAP molecular marking method for genetic diversity analysis of tetracera asiatica

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Embodiment Construction

[0059]In order to express the present invention more clearly, the present invention will be further described below through specific examples. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0060] (1) Experimental materials

[0061] 90 parts of Tinleaf vines used in this test were collected from Guangxi, Guangdong, Hong Kong, etc., see Table 1 for details.

[0062] Table 1 Test material numbers and sampling points

[0063]

[0064]

[0065]

[0066] (2) SRAP primer design

[0067] The 9 pairs of primer combinations used in the present invention are all fluorescently labeled and synthesized by Beijing Dingguo Changsheng Biotechnology Co., Ltd. After the primers are synthesized, centrifuge and then dilute and shake to dissolve the primers as required. Dilute the primers to 10 μM ...

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Abstract

The invention provides an SRAP molecular marking method for genetic diversity analysis of tetracera asiatica, and belongs to the technical field of molecular biology. According to the method, an SRAPmolecular marker primer system of tetracera asiatica is established, and the primer system comprises nine specific primer pairs; then the primer system is used for carrying out SRAP-PCR reaction amplification on a tetracera asiatica sample, genetic similarity and genetic distance are calculated according to a DNA random amplification result, clustering analysis is carried out, and a clustering graph is constructed. Amplified fragments obtained by using the screened primers are good in repeatability and clear in strip and exhibit high polymorphism; and meanwhile, the SRAP molecular marker primer system is used for carrying out clustering analysis on tetracera asiatica, a new method is established for variety identification and genetic diversity analysis of tetracera asiatica, and technicalguidance and theoretical support are provided for scientific research of resource identification, genetic diversity analysis and the like of tetracera asiatica.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a SRAP molecular marker method used for the analysis of the genetic diversity of the vine. Background technique [0002] Tin leaf vine is the root of Tetracera asiatica (Lour.) Hoogland. It is cool in nature, bitter in taste, has the functions of restraining diarrhea, reducing swelling and relieving pain. Commonly used in the treatment of diarrhea, dysentery, proctoptosis and other diseases. Its medicinal materials are rich in tannins, flavonoids, terpenoids and other biologically active ingredients, which have obvious antibacterial, anti-inflammatory, anti-tumor activities [State Administration of Traditional Chinese Medicine Chinese Materia Medica Editorial Committee. Zhonghua Materia Medica [M ]. Volume 3. Shanghai: Shanghai Science and Technology Press, 1999: 510-511.]. Tin leaf vine is rich in medicinal resources, and is produced in southern and southwestern pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 朱华笪舫芳戴忠华张淼樊柳园李鹏何瑞婷
Owner GUANGXI UNIV OF CHINESE MEDICINE
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