Ultraviolet mutagenic obtained low temperature salina and its identifying method

An identification method and a low temperature-resistant technology, applied in the field of salina

Inactive Publication Date: 2007-04-11
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing salt algae can only be cultivated in tropical or subtropical areas with high temperature, to provide a low-temperature-resistant mutant strain of salina salina, and to adopt ultraviolet mutagenesis and low-temperature breeding of salina salina. Technical route and identification method

Method used

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  • Ultraviolet mutagenic obtained low temperature salina and its identifying method
  • Ultraviolet mutagenic obtained low temperature salina and its identifying method
  • Ultraviolet mutagenic obtained low temperature salina and its identifying method

Examples

Experimental program
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Effect test

Embodiment 1

[0046] For ultraviolet mutagenesis and cultivation of low-temperature-resistant salt algae, it is necessary to prepare artificial seawater culture medium for cultivating Dunaliella bardawil, and divide the components into 50-1000 times mother liquid in advance, and then dilute it as needed to make culture liquid. . The composition of the artificial seawater culture solution for preparing Salina salina is shown in Table 1. The artificial seawater culture solution was divided into 100mL Erlenmeyer flasks, each containing 20mL, tightly plugged with cotton plugs, and wrapped with kraft paper. Place the packaged above-mentioned culture solution in an autoclave, and sterilize at 121° C. and a pressure of 0.1 mPa for 15 minutes. Take out the sterilized culture medium, cool it to room temperature, inoculate 1mL salina algae liquid into the sterile culture medium, shake it gently, and plug it with a cotton plug. In an incubator at 20°C, it was illuminated at 4000lx for 12h and dark f...

Embodiment 2

[0051] Similar to Example 1, the difference is that the artificial seawater culture solution for cultivating Dunaliella salina is prepared, the artificial seawater culture solution is divided into 250mL Erlenmeyer flasks, each bottle is filled with 50mL, sealed with 8 layers of gauze, and wrapped with kraft paper. Place the packaged above-mentioned culture solution in an autoclave, and sterilize at 121° C. and a pressure of 0.1 mPa for 20 minutes. Take out the sterilized culture medium, cool it to room temperature, inoculate 5mL salina algae liquid into the sterile culture medium, shake it gently, and seal it with 8 layers of gauze. In an incubator at 30°C, the light is 12h, 2000lx every day, and the darkness is 12h. Continuously cultured for 5 days to obtain synchronized growth of Salina salina, that is, a large number of cell divisions occurred in Salina salina from 6 hours after dark culture to 2 hours after light culture. Take out 0.5mL of the algae liquid regularly the ...

Embodiment 3

[0056] Similar to Example 1, the difference is that the artificial seawater culture solution for cultivating Dunaliella bardawil is prepared, the artificial seawater culture solution is divided into 150mL Erlenmeyer flasks, each bottle has a capacity of 30mL, the cotton plug is tightly plugged, and the kraft paper. Place the packaged above-mentioned culture solution in an autoclave, and sterilize at 121° C. and a pressure of 0.1 mPa for 15 minutes. Take out the sterilized culture medium, cool it down to room temperature, inoculate 3 mL of salina algae liquid into the sterile culture medium, shake it gently, and plug it with a cotton plug. In an incubator at 25°C, the light is 12h, 3000lx every day, and the darkness is 12h. Continuously cultured for 3 days to obtain synchronized growth of Salina salina, that is, a large number of cell divisions occurred in Salina salina from 6 hours after dark culture to 2 hours after light culture. Take out 5mL of the algae liquid regularly ...

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Abstract

The present invention relates to saline algae, provides one kind of low temperature tolerant saline algae and its ultraviolet mutagenesis breeding process and identifying method. The low temperature tolerant saline algae features its light illuminating growth at 3-10deg.c to reach (1.05-8.11)x10<6> cell/ml in the cell density 2.1-21 times higher than the contrast; the genetic similarity coefficient to wild strain of 0.680-0.910; and increased or decreased protein zone(s) compared with wild strain.

Description

technical field [0001] The invention relates to a salina, in particular to a method for mutagenizing the salina (Dunaliella bardawil) by ultraviolet rays, obtaining a mutant strain capable of enduring low temperature (3-10° C.) through selection and identification thereof. Background technique [0002] Salina (Dunaliella sp.) is a type of unicellular eukaryotic green algae, which naturally has no cell wall and is easily digested. Salina cells have a low ash content and are rich in glycerin, protein, β-carotene, carbohydrates and a certain amount of unsaturated fatty acids and vitamins. It is one of the ideal health foods and can also be used as high-quality bait and feed for animals , so it has become one of the main cultured microalgae for humans, and has high utilization value in commercial development. The optimum growth temperature of Salina is 25-30°C. When the temperature is lower than 10°C, Salina grows slowly, or even does not grow, forming cyst cells. The temperat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N1/12C12Q1/04C12Q1/68
Inventor 刘广发周韬
Owner XIAMEN UNIV
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