Method for breeding beet male sterile line and maintainer line by using excellent pollination line

A male sterile line and male sterile technology, applied in the fields of botany equipment and methods, applications, plant genetic improvement, etc., can solve the labor-intensive and time-consuming problems of breeding

Pending Publication Date: 2022-07-05
石河子农业科学研究院
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Problems solved by technology

At present, the conventional method of plant-to-plant pair hybridization and then backcrossing is generally used in China to breed CMS and O-type lines of sugar beet. It takes at least 7 to 10

Method used

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  • Method for breeding beet male sterile line and maintainer line by using excellent pollination line

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Embodiment 1

[0026] A kind of method step of utilizing excellent pollination line to select male sterile line and maintainer line of sugar beet of the present invention is as follows:

[0027] 2001: Select the O-type line (O) with excellent traits and the pollinator line (P) with excellent traits, respectively, and cultivate the mother roots in the autumn of that year, and cultivate 80-100 mother roots per material.

[0028] 2002: In the spring of the concentrated isolation area, one O-type (O) parent root and one pollinator (P) parent root were planted together in pairs, and the stamens on the O-type plant were manually removed before flowering. To ensure that it can only accept pollen from the pollinating line (P), the pair of materials is then placed in the same isolation hood, and artificially assisted pollination is performed when the pollinating plant in the isolation hood blooms; this will produce 80 to 100. isolation cover. When the seeds are mature, in each isolation hood, the Ox...

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Abstract

The invention provides a method for breeding a beet male sterile line and a maintainer line by using an excellent pollination line, which comprises the following steps: hybridizing an excellent single-grain beet maintainer line with a beet pollination line to obtain O * P seeds; selecting a single plant from the O * P filial generation as a male parent, performing plant-to-plant test cross on the male parent and a beet male sterility test line, and separately harvesting seeds to obtain CMS-n * (O * P)-n and (O * P)-n seeds; sowing the CMS-n * (O * P)-n seeds to cultivate mother roots, and investigating fertility during flowering; sowing seeds harvested from a male parent single plant of which the offspring is completely sterile in CMS plant-to-plant test cross, cultivating a mother root, independently isolating, opening and pollinating, and harvesting the seeds in a mixed manner; obtaining a beet maintainer line; a CMS test line used for test cross is a male sterile line. By adopting the method, excellent characters in the pollination line can be introduced into the O-type line, the O-type line is improved within a short time, and the CMS and the O-type line are rapidly bred.

Description

technical field [0001] The invention belongs to the technical field of sugar beet breeding, and in particular relates to a method for selecting a male sterile line and a maintainer line of sugar beet by utilizing an excellent pollination line. Background technique [0002] At present, the commercial varieties used in sugar beet production at home and abroad are single-seed male sterile three-cross hybrids. The most critical link in the production of commercial sugar beet hybrids is the selection of single-seed CMS (male sterile line) and O-type line (maintenance line). nurture. Since sugar beet is a biennial crop under normal circumstances, root vegetative growth is carried out in the first year, and the tuberous roots after low temperature vernalization are planted into the field in the second year for reproductive growth and flowering and seeding. At present, the conventional method of plant-to-plant pairwise hybridization and backcrossing is widely used in China to selec...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 李蔚农刘长兵袁团团刘洪亮崔瑜王荣华汤晓昀丁兆斐李守明艾依肯
Owner 石河子农业科学研究院
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