Breeding method for rapidly introducing rice target characters

A technology for target traits and rice, applied in botany equipment and methods, biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of long breeding cycle and heavy workload, and achieve faster breeding speed, The effect of high genetic similarity

Inactive Publication Date: 2017-04-26
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the F1 generation, select true hybrids to backcross with recurrent parents to obtain BC1F1, and then backcross multiple generations (more than 4 generations) to obtain high-generation materials; then, from BC5F2 onwards, select strains that carry target traits and are similar to the parents ; The breeding cycle is long and the workload is heavy, which has become a bottleneck for the innovation of rice germplasm resources

Method used

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  • Breeding method for rapidly introducing rice target characters
  • Breeding method for rapidly introducing rice target characters
  • Breeding method for rapidly introducing rice target characters

Examples

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Embodiment

[0018] Breeding objective: Introduce the trait of purple endosperm and black seed coat into the 93-11 restorer line.

[0019] Breeding process: 1) Using the purple endosperm (resource of our research group) carrying the black seed coat trait as the female parent, cross with the reincarnated parent 93-11 to obtain hybrid F1; Perform hybridization to obtain the BC1F1 population; 2) Use 48 molecular markers evenly distributed on the 12 rice chromosomes in the "Technical Regulations for Rice Variety Identification SSR Marker Method" to conduct genetic similarity analysis on the individual plants of the BC1F1 population, and screen for genes carrying target traits And compared with 93-11, there are 6 BC1F1 single plants whose genetic similarity is higher than 90%. The strains are: 1, 2, 5, 6, 7, and 10, as shown in Table 1; (3) 93-11 is used as the mother In this case, the selected BC1F1 single plant was backcrossed, and a line with black seed coat traits and economic traits simila...

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Abstract

The invention discloses a breeding method for rapidly introducing rice target characters. The method includes the following steps that rice resources carrying target characters serve as a male parent or a female parent and are hybridized with a recurrent parent to obtain a hybrid F1; the recurrent parent serves as a female parent to be hybridized with the hybrid F1 to obtain a BC1F1 group; according to 48 molecular markers distributed evenly on 12 chromosomes of rice in the SSR labeling method of a rice variety identification technical regulation, genetic similarity analysis is conducted on a single plant of the BC1F1 group, and a BC1F1 single plant is screened, wherein the BC1F1 single plant carries the target characters and the genetic similarity is higher than 90% compared with the recurrent parent; the recurrent parent serves as a female parent to be hybridized with the BC1F1 single plant, and a plant carrying the target characters and similar to the recurrent parent is bred and selected from later generations. Through the comparison of SSR molecular markers with the recurrent parent, when the genetic similarity is high, the target characters are introduced rapidly and more-efficiently, and the genetic resource innovative breeding speed is greatly increased.

Description

technical field [0001] The invention relates to the field of rice breeding, in particular to a breeding method for quickly introducing target traits into rice. Background technique [0002] To introduce the target traits of rice, the pedigree method is generally used at present, and multi-generation backcross selection is required. In the F1 generation, select true hybrids to backcross with recurrent parents to obtain BC1F1, and then backcross multiple generations (more than 4 generations) to obtain high-generation materials; then, from BC5F2 onwards, select strains that carry target traits and are similar to the parents ; The breeding cycle is long and the workload is heavy, which has become a bottleneck for the innovation of rice germplasm resources. Contents of the invention [0003] In order to solve the above problems, the present invention provides a breeding method for quickly introducing target traits in rice. The target traits can be quickly and efficiently intro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/68
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张伟张德文汪婉琳杨前进
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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