Specificity test approximate variety effective screening method based on genetic similarity

Abstract

The invention discloses a specificity test approximate variety effective screening method based on genetic similarity. The method can be used for efficiently screening plant approximate varieties of wheat, corn, Chinese cabbages, soybeans and the like. The method is characterized in that firstly, for application varieties and known varieties, the DNA fingerprints of a group of SSR (simple sequencerepeat) markers or SNP (single nucleotide polymorphism) markers are collected; then, a DNA fingerprint database system of the known varieties is built; approximate variety screening database softwareis developed; the genetic similarity of the application varieties and the known varieties can be automatically calculated by the software; the approximate variety of each application variety can be selected according to any genetic similarity threshold value. A database technology is used for managing variety DNA fingerprint data; the genetic similarity is calculated; the approximate variety is selected; the work efficiency is greatly improved; the completion time of the approximate variety screening is shortened from several days to moment; the defects of approximate variety selection operation complexity, long consumed time and high error rate of the existing method are overcome.

Description

technical field [0001] The invention relates to a genetic similarity-based specificity testing method for screening similar varieties, which belongs to the technical field of new plant variety testing (or called DUS testing). Background technique [0002] Specificity, consistency and stability are the basic requirements for plant varieties, and are the prerequisites for new plant varieties to obtain variety rights protection, major crop variety approval and non-main crop variety registration. Specificity means that the variety (applied variety) for which the protection of the variety right is applied for or approved or registered shall be clearly different from all known varieties of the same genus or species (known varieties) before the filing date. To identify a variety as having specificity, it needs to be demonstrated that the variety is phenotypically distinct from any other known variety of the same crop. There are often tens of thousands of known varieties of major c...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems in the prior art that the selection of similar varieties is cumbersome, time-consuming, and error-prone due to manual operation, the present invention provides a method for screening similar varieties based on genetic similarity specificity testing, which can realize wheat, corn, Chinese cabbage Fast, efficient and accurate screening of similar plant varieties such as , soybean and so on

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