Immobilization method of salt-tolerant and cold-tolerant compound strain and application of immobilization method

Abstract

The invention discloses an immobilization method of a salt-tolerant and cold-tolerant compound strain and an application of the immobilization method. The immobilization method of the salt-tolerant and cold-tolerant compound strain comprises the following steps: firstly screening efficient anaerobic ammonia oxidation bacteria, denitrifying bacteria and petroleum-degrading bacteria, training salt tolerance and cold tolerance to respectively prepare trained Kuenenia. Anammoxidans, Ochrobactrum.spand Flavobacterium mizutaii; putting prepared biological carbon spheres into a salt-tolerant, cold-tolerant, efficient and compound degrading bacteria enrichment solution, culturing films for the biological carbon spheres and subsequently mixing the degrading bacteria enrichment solution with a salt-tolerant, cold-tolerant, efficient and compound degrading bacteria embedding compound solution, finally putting the biological carbon spheres in the mixed solution into a saturated borate solution and a 2%CaCl2 mixed solution one by one, then putting the biological carbon spheres into a refrigerator, crosslinking an washing to obtain salt-tolerant and cold-tolerant compound strain immobilization biological carbon spheres. The salt-tolerant and cold-tolerant compound strain is applied to purification of ammonia nitrogen and water polluted by petroleum hydrocarbon under the condition with low temperature and high salt, so that the strain is high in content of microbial biomass; obviously, the biological carbon spheres are used as carriers for immobilizing the salt-tolerant and cold-tolerant compound strain; the carriers are wide in material resources, simple to manufacture, suitable for industrialization, high in mechanical strength, excellent in treatment effect, low in cost and free of secondary pollution to the environment.

Description

technical field [0001] The invention relates to an immobilization method and application of a salt-resistant and cold-resistant compound bacterial strain, which specifically utilizes the immobilized anaerobic ammonium oxidation, denitrification and petroleum-degrading bacteria mixed in a certain proportion to jointly degrade ammonia nitrogen and petroleum under low-temperature and high-salt conditions hydrocarbon. Background technique [0002] At present, the water bodies of some rivers in our country are seriously polluted by ammonia nitrogen and petroleum hydrocarbons, which make the water bodies stink and destroy habitats. The reason why it is difficult to treat natural water bodies polluted by petroleum hydrocarbons and ammonia nitrogen is that petroleum hydrocarbons inhibit the oxygen enrichment of water bodies, which reduces the activity of aerobic microorganisms or dies. The commonly used methods for ammonia nitrogen and petroleum hydrocarbon pollution include physic...

AI Technical Summary

Problems solved by technology

Low-temperature oil-degrading bacteria strains, culture methods, culture media and their applications have been studied (ZL201010239621.7). The oil-degrading bacteria screened out by this method have a higher degradation rate than 40%, but cannot adapt to high salinity environment