Protein related to cold resistance of plant, coding genes and application thereof

A technology encoding genes and cold tolerance, applied to proteins related to plant cold tolerance and its encoded genes and application fields, can solve problems such as genetic bottlenecks, achieve simple operation, improve cold tolerance, and be suitable for popularization and application

Inactive Publication Date: 2009-04-22
CHINA AGRI UNIV
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Problems solved by technology

According to statistics, the number of alleles in cultivated rice is about 60% of wild rice (Sun C Q, Wang X K, Li Z C, Yoshimura A. Comparison of the genetic diversity of common wild rice (Oryza rufipogon Griff.) and cultivated rice (O. sativa L.) using RFLP markers. Theor Appl Genet, 2001, 102: 157-162), which leads to the genetic bottleneck problem faced by the current rice variety breeding

Method used

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  • Protein related to cold resistance of plant, coding genes and application thereof
  • Protein related to cold resistance of plant, coding genes and application thereof
  • Protein related to cold resistance of plant, coding genes and application thereof

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Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1, the acquisition of the gene LTT7 that improves the cold tolerance of rice bud stage

[0040] First, the Jiangxi Dongxiang common wild rice was backcrossed and self-crossed with the ultra-high-yield rice variety Guichao 2, and a high-generation backcross population (BC) with Guichao 2 as the genetic background was constructed. 4 f 2 group), the cold tolerance of this group was identified at the bud stage, and Lijiang Xintuan Heigu was used as a control. The specific identification method is as follows: soak the seeds of Guichao 2, the high-generation backcross population and Lijiang Xintuan Heigu with 5% (volume percentage) sodium hypochlorite solution for 20 minutes, then rinse with water for 3-4 times, 37 Soak the seeds at ℃ for 1 day, then place the seeds on filter paper soaked in water in glass test tubes, and put the test tubes into the light cultivation room (day temperature 28 ℃, night temperature 25 ℃, light and dark time of 12 hours each day, rela...

Embodiment 2

[0055] Embodiment 2, the acquisition of LTT7 transgenic rice and its cold tolerance identification

[0056] 1. Construction of LTT7 plant expression vector

[0057] The 836bp DNA fragment of the LTT7 gene amplified in the above-mentioned embodiment 1 is cloned into the plant expression vector pCAMBIA1300-35S (this carrier is that the 35S promoter on the PBI121 (Promega company) is utilized endonuclease HindIII and XbaI (TaKaRa company, Numbers are D1060A, D1093A) after digestion and recovery, recombination into the pCAMBIA1300 vector obtained) between the KpnI and SacI restriction sites of the multi-cloning site, a recombinant expression vector containing the rice LTT7 gene was obtained, named pCAMBIA1300-35S- LTT7.

[0058] 2. Obtaining of LTT7 transgenic rice

[0059] The recombinant expression vectors pCAMBIA1300-35S-LTT7 and pCAMBIA1300-35S constructed in step 1 were respectively transformed into mature embryo calli of Nipponbare by particle gun method, and two rounds of...

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Abstract

The invention provides a protein related to the cold tolerance of plants and an encoding protein and application thereof. The protein is protein of the following (a) or (b): (a) the protein consisting of an amino acid sequence shown in sequence 1 in a sequence table; and (b) the protein which is obtained through the substitution and / or deficiency and / or adding of one or a plurality of amino acid residues of the amino acid sequence of the sequence 1 in the sequence table, can improve the cold tolerance of the plants, and is derived from the (a). The method for cultivating cold-tolerant plants has important theoretical and practical significances on the research of molecular mechanism of the cold tolerance of the plants, the breeding of cold-tolerant species and the molecular breeding of the cold tolerance of the plants, and provides an economical, quick and effective approach for improving the cold tolerance of the plants. The invention has broad application and market prospect in the agricultural field.

Description

technical field [0001] The invention relates to a protein related to plant cold tolerance, its coding gene and application. Background technique [0002] Rice is an important food crop, and chilling injury at the germination stage is one of the important factors affecting rice production in the early rice planting areas in the middle and lower reaches of the Yangtze River, the northeast and northwest rice areas, and the one-crop rice area in the Yunnan-Guizhou Plateau. If the rice seedlings suffer from chilling damage during the germination stage, the growth of rice seedlings will be slowed down and the tillers will be reduced. In severe cases, there will even be a large area of ​​stiff seedlings and dead seedlings, which will eventually lead to a significant reduction in rice yield. Rice varieties. Common wild rice is the ancestor of cultivated rice in Asia. During the evolution of wild rice into cultivated rice, through natural selection and artificial selection, the gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N15/82C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H1/00A01H5/00
Inventor 孙传清刘凤霞苏震谭禄宾刘家勇付永彩朱作峰
Owner CHINA AGRI UNIV
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