Method used for identifying cold tolerance of rice at booting stage

A technology of cold tolerance and booting stage, applied in the field of crop genetics and breeding, can solve the problems of high cost, rice pollen abortion, low seed setting rate, etc., and achieve the effect of high accuracy and low cost

Active Publication Date: 2015-03-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artificial climate chamber treatment is not only costly, but also difficult to screen and identify large quantities of materials
Other methods are susceptible to outside air temperature
In a word, the identification of cold tolerance is easily affected by the environment. Different environments ...

Method used

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  • Method used for identifying cold tolerance of rice at booting stage
  • Method used for identifying cold tolerance of rice at booting stage
  • Method used for identifying cold tolerance of rice at booting stage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, utilize cold water pond method to identify the cold tolerance of strain

[0033] (1) Test materials

[0034] Towada: It is a cold-sensitive parent, which can be obtained from rice research institutions, such as the core germplasm bank of China Agricultural University, Yunnan Academy of Agricultural Sciences, and China Rice Research Institute.

[0035] NIL1913: It is a near-isogenic line containing a cold-tolerant QTL, that is, a cold-tolerant line; it is derived from the high-generation backcross progeny of the strong cold-tolerant parent Kunming Xiaobaigu and the cold-sensitive parent Towada. The specific backcrossing method is to start from the hybrid F2 generation, randomly select 10-20 plants from the separated materials of each generation to backcross with Towada respectively, and judge whether to keep the hybrid combination according to the self-crossing panicle setting rate on the same plant at maturity. Generally, the seed setting rate is relativel...

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Abstract

The invention provides a method used for identifying cold tolerance of rice at a reproductive stage. The method provided by the invention comprises the following steps: 1) building a pool; 2) controlling the temperature of water in the pool; and 3) carrying out cold treatment on experimental materials. With the method, cold tolerance of different rice strains are identified, and identification results show that the method provided by the invention can effectively identify cold tolerance of different experimental materials at a booting stage. The method provided by the invention has the advantages of insusceptibility to weather conditions, easy treatment of large quantities of experimental materials at the same time, low cost and high accuracy and facilitates utilization of cold tolerance breeding and identification of cold-tolerant genetic materials at the reproductive stage of rice and assistant selection of varieties with cold tolerance at the booting stage.

Description

technical field [0001] The invention belongs to crop genetics and breeding, in particular to a method for identifying the cold tolerance of rice at the booting stage. Background technique [0002] Rice is one of the main food crops in my country and the world, and is the staple food for more than 60% of the population in my country. The world's rice planting area is about 140 million hectares 2 , due to abnormal global climate change and frequent occurrence of extreme climate, there are 15 million hm 2 The above rice cultivation is threatened by low temperature. Rice is sensitive to low temperature at the booting stage, and japonica rice and indica rice will have severe chilling injury below 15°C and 18°C, respectively. Therefore, correctly evaluating the cold tolerance of rice during the reproductive growth period at low temperature is the key to breeding new cold-tolerant rice varieties. [0003] The methods for identification of cold resistance at the booting stage in...

Claims

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Application Information

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IPC IPC(8): A01G16/00A01C1/00A01C7/00A01G22/22
CPCA01G7/06A01G22/00A01G22/22
Inventor 李自超潘英华李金杰张洪亮余建平李继龙李刚岭
Owner CHINA AGRI UNIV
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