Method used for identifying cold tolerance of rice at booting stage

Abstract

The invention provides a method used for identifying cold tolerance of rice at a reproductive stage. The method provided by the invention comprises the following steps: 1) building a pool; 2) controlling the temperature of water in the pool; and 3) carrying out cold treatment on experimental materials. With the method, cold tolerance of different rice strains are identified, and identification results show that the method provided by the invention can effectively identify cold tolerance of different experimental materials at a booting stage. The method provided by the invention has the advantages of insusceptibility to weather conditions, easy treatment of large quantities of experimental materials at the same time, low cost and high accuracy and facilitates utilization of cold tolerance breeding and identification of cold-tolerant genetic materials at the reproductive stage of rice and assistant selection of varieties with cold tolerance at the booting stage.

Description

technical field [0001] The invention belongs to crop genetics and breeding, in particular to a method for identifying the cold tolerance of rice at the booting stage. Background technique [0002] Rice is one of the main food crops in my country and the world, and is the staple food for more than 60% of the population in my country. The world's rice planting area is about 140 million hectares 2 , due to abnormal global climate change and frequent occurrence of extreme climate, there are 15 million hm 2 The above rice cultivation is threatened by low temperature. Rice is sensitive to low temperature at the booting stage, and japonica rice and indica rice will have severe chilling injury below 15°C and 18°C, respectively. Therefore, correctly evaluating the cold tolerance of rice during the reproductive growth period at low temperature is the key to breeding new cold-tolerant rice varieties. [0003] The methods for identification of cold resistance at the booting stage in...

AI Technical Summary

Problems solved by technology

Artificial climate chamber treatment is not only costly, but also difficult to screen and identify large quantities of materials

Other methods are susceptible to outside air temperature

In a word, the identification of cold tolerance is easily affected by the environment. Different environments (weather conditions) require different treatment conditions. If the treatment is too heavy, it will lead to abortion of rice pollen, low seed setting rate, and no cold-tolerant rice materials can be screened out; light, it will lead to high seed setting rate of rice, no difference between materials

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