Clone of corn phosphatidylinositol transportprotein gene ZmSEC14p and application thereof

A technology of phosphatidylinositol and transporter, applied in the field of molecular biology and biology, to achieve the effect of improving cold resistance
CN104818286AInactive Publication Date: 2015-08-05JILIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JILIN UNIV
Publication Date
2015-08-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the clone of corn phosphatidylinositol transportprotein gene ZmSEC14p and an application thereof and belongs to the technical field of molecular biology and biotechnology. The nucleotide sequence of the cold-tolerant corn selfing line W9816 phosphatidylinositol transportprotein gene ZmSEC14p is shown in SEQ ID NO:1; the cold-tolerant corn selfing line W9816 phosphatidylinositol transportprotein is coded by the ZmSEC14p gene and the amino acid sequence is shown in SEQ ID NO:2; a plant expression vector contains the cold-tolerant corn selfing line W9816 phosphatidylinositol transportprotein gene ZmSEC14p; the plant expression vector is constructed and transformed into arabidopsis thaliana, and the life states of the transgenic Arabidopsis, such as the phenotype and the survival rate, are observed through low temperature and freeze stress; the experiment result shows that the cold-tolerant corn selfing line W9816 phosphatidylinositol transportprotein gene ZmSEC14p is capable of increasing the cold resistance of the arabidopsis.
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Description

technical field

[0001] The invention belongs to the field of molecular biology and biotechnology, and specifically relates to the cloning and application of a phosphatidylinositol transporter gene ZmSEC14p of a cold-tolerant corn inbred line W9816. Background technique

[0002] Crops are often subject to various abiotic stresses, such as drought, salt, low temperature, etc., which seriously restrict the planting area of ​​crops and even lead to a decline in yield [1] . Low temperatures affect seed germination, seedling growth, and agricultural yields. In order to resist or adapt to adversity, the plant senses the changes in the external environment and transmits signals to the cells through a variety of channels, induces the expression of some response genes, produces some functional proteins, osmotic adjustment substances and transmission Transcription factors that signal and regulate gene expression. Many genes that respond to low temperature have been reported, such as...

Claims

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