Method for breeding low-temperature-tolerant strain of Argopecten irradias hybrid-backcrossed scallops

A technology of low temperature resistance and scallops, applied in fish farming, application, climate change adaptation, etc., can solve the problems of small size of commercial shellfish, degradation of germplasm, short lifespan, etc., and achieve simple and convenient operation, fast growth speed, and wide application Effect

Active Publication Date: 2016-11-09
QINGDAO AGRI UNIV
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Problems solved by technology

However, due to its own small commodity size (about 5-6 cm), short life span (about 13 months) (Dall., 1909; Minchin., 2003), and long-term sterility after introduction, resulting in breeding quality degradation, resulting in smaller and smaller commercial shellfish (Zhang Guofan et al., 2003), high mortality rate in the breeding process, for example, in 2007, the bay scallop mortality rate was as high as about 70%, which has seriously restricted the development of the bay scallop aquaculture industry in our province. develop
In response to this phenomenon, domestic shellfish breeders have improved the germplasm of bay scallops through introduction and rejuvenation, and achieved some results, but failed to fundamentally solve the problem of germplasm degradation.

Method used

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Embodiment 1

[0025] 1) At the end of March 2010, mature purple scallops were harvested, and purple scallop sperm were obtained after temperature stimulation. After insemination with bay scallop eggs, the first generation seedlings of purple scallop (sperm) and bay scallop (egg) hybrids were obtained, and adult shellfish were obtained.

[0026]2) In the spring of 2011, the scallops of the above-mentioned first generation of hybrids will be matured and selected from the scallops that are large in size and only develop in the female area. After being dried in the shade and stimulated by temperature, eggs will be obtained, and the sperm of the bay scallops will be fertilized to cultivate the first generation of hybrids ( ovum) and bay scallop (sperm) backcross sea hybrid (BC 1 ) base groups.

[0027] 3) By the end of January 2012, BC 1 When growing to 10 months old, healthy adult shellfish were selected to continue low-temperature stress, the stress temperature was controlled at half 0°C, and...

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Abstract

The invention relates to a method for breeding low-temperature-tolerant strain of Argopecten irradias hybrid-backcrossed scallops, characterized by including: selecting individuals that are high in growth speed and tolerance to low temperatures from Argopecten irradias and Argopecten purpuratus hybrid first-generation family line or population as breed scallops; backcrossing the individuals having high tolerance to low temperatures in the hybrid first generation with semen of Argopecten irradias to establish a backcrossed family line or population; finally, performing breeding of low-temperature-tolerant breeding core population at least three times to develop a low-temperature-tolerant strain. The Argopecten irradias and Argopecten purpuratus hybrid first-generation high in growth speed and significant in hybridization advantage is used as a female parent that is backcrossed with Argopecten irradias (male parent) to gather cold-tolerant genes of Argopecten irradias to the Argopecten irradias hybrid backcrossed first-generation, the individuals having high tolerance to low temperatures in the Argopecten irradias hybrid backcrossed first-generation are used as a breeding core population, the breed core population is subjected to continuous three generations of low-temperature-tolerant breeding, and the strain can tolerate a low temperature down to 0 DEG C and grows 15% higher than Argopecten irradias. It is apparent that the method is simple and convenient to perform and is widely applicable to the breeding of low-temperature-tolerant strains of hybrid scallops.

Description

technical field [0001] The invention belongs to the breeding technology of shellfish aquaculture, and in particular relates to a method for cultivating low-temperature resistant strains of sea hybrid backcross scallops. Background technique [0002] The bay scallop (Argopecten irradians irradians) is native to the Atlantic coast of the United States and was introduced to my country in 1982. Due to its fast growth, short breeding cycle and wide temperature tolerance range (-1-31°C), the scallop has become the main cultured species in northern my country (Zhang Fusui, 2000), and its output once reached more than 800,000 tons in 2005. However, due to its own small commodity size (about 5-6 cm), short life span (about 13 months) (Dall., 1909; Minchin., 2003), and long-term sterility after introduction, resulting in breeding quality degradation, resulting in smaller and smaller commercial shellfish (Zhang Guofan et al., 2003), high mortality rate in the breeding process, for exa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘博王春德刘晓刘凤巧刘桂龙马斌
Owner QINGDAO AGRI UNIV
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