Preparation method of R-polysaccharide fermentation liquor and preparation method of composite preservative of R-polysaccharide fermentation liquor

A technology of composite preservatives and fermented liquid, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of complex preparation process, high production cost, low yield, etc., and achieve simple process and food safety High, clean and hygienic environment

Inactive Publication Date: 2011-02-16
王心礼
View PDF2 Cites 25 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problems of complex preparation process of existing R-polysaccharide, high production cost and low y

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0011] Specific embodiment one: the preparation method of present embodiment R-polysaccharide fermented liquid is carried out according to the following steps: one, bacillus subtilis, streptococcus thermophilus and brewer's yeast are respectively inoculated in culture medium by 5% inoculum size, in 30 Cultivate in a constant temperature incubator for 48 hours to obtain the activated strains of the three bacteria, collect the three kinds of bacteria after centrifugation, and then wash three times with sterilized phosphate buffer solution; 2. Press the washed three kinds of bacteria The bacterial cells were mixed at a ratio of 1:1:1, then inoculated in a fermenter with a fermentation medium, and fermented for 48 hours at a fermentation temperature of 30±1°C and a shaker speed of 120r / min to obtain a fermentation broth 3. After the fermentation liquid is purified, the preparation of the R-polysaccharide fermentation liquid is completed.

[0012] The R-polysaccharide fermented liq...

specific Embodiment approach 2

[0036] Specific embodiment two: the difference between this embodiment and specific embodiment one is that Bacillus subtilis is purchased from the China Agricultural Microorganism Culture Collection Center (ACCC) in step one, and the strain preservation number is 01185, and the preservation date is February 25, 2002 day. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0037] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the Streptococcus thermophilus in step 1 was purchased from the China Agricultural Microorganism Culture Collection Center (ACCC), the strain preservation number is 10213, and the preservation date is 2006 June 20. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses to a preparation method of R-polysaccharide fermentation liquor and a preparation method of a composite preservative of the R-polysaccharide fermentation liquor, relating to a preparation of fermentation liquor and a preparation method of a composite preservative of the fermentation liquor, which solves the problems of complex preparation technology of the traditional R-polysaccharide preparation, and high production cost and low yield of the preparation of a preservative of the traditional R-polysaccharide preparation. The preparation method of the R-polysaccharide fermentation liquor comprises the following steps of activating bacillus subtilis, streptococcus thermophilus and brewers' yeast; collecting and washing after being centrifuged; fermenting and cultivating the thalluses after mixing; and purifying the fermentation liquor. The preparation method of the preservative is as follows: mixing nisin, natamycin, propionic acid, antioxidant of bamboo-leaf, chitosan, ethylene diamine tetraacetic acid (EDTA), cellulose, ethanol, sorbitol and oxygen-enriched active water; and mixing the mixed liquor and the R-polysaccharide fermentation liquor to be reacted. The preparation of the R-polysaccharide fermentation liquor has the advantages of simple technology, no three-waste generation, no pollution discharge and no peculiar smell. The R-polysaccharide fermentation liquor is prepared into a composite preservative, the production cost is ** yuan/ton, and the yield is high.

Description

technical field [0001] The invention relates to a preparation method of a fermented liquid and a compound preservative thereof. Background technique [0002] R-polysaccharide is a natural food preservative, which has a wide range of bactericidal and antibacterial abilities and is very effective. The antibacterial mechanism is to establish a mode of enzyme biosynthesis and activity regulation, and form micropores on the cell membrane of microorganisms, resulting in increased membrane permeability and The energy system is destroyed, so it can quickly kill the microorganisms in the food, and inhibit a small amount of microorganisms in the food to avoid their reproduction, so as to achieve the purpose of antiseptic preservation. [0003] At present, the preparation process of R-polysaccharide is complicated, and a large amount of sewage treatment and other technologies are required in the production, and chemical synthesis raw materials are often used, which is easy to produce p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23L3/3562C12P19/04C12R1/46C12R1/865C12R1/125C12P39/00
Inventor 王心礼
Owner 王心礼
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap