Preparation method of R-polysaccharide fermentation liquor and preparation method of composite preservative of R-polysaccharide fermentation liquor
A technology of composite preservatives and fermented liquid, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of complex preparation process, high production cost, low yield, etc., and achieve simple process and food safety High, clean and hygienic environment
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specific Embodiment approach 1
[0011] Specific embodiment one: the preparation method of present embodiment R-polysaccharide fermented liquid is carried out according to the following steps: one, bacillus subtilis, streptococcus thermophilus and brewer's yeast are respectively inoculated in culture medium by 5% inoculum size, in 30 Cultivate in a constant temperature incubator for 48 hours to obtain the activated strains of the three bacteria, collect the three kinds of bacteria after centrifugation, and then wash three times with sterilized phosphate buffer solution; 2. Press the washed three kinds of bacteria The bacterial cells were mixed at a ratio of 1:1:1, then inoculated in a fermenter with a fermentation medium, and fermented for 48 hours at a fermentation temperature of 30±1°C and a shaker speed of 120r / min to obtain a fermentation broth 3. After the fermentation liquid is purified, the preparation of the R-polysaccharide fermentation liquid is completed.
[0012] The R-polysaccharide fermented liq...
specific Embodiment approach 2
[0036] Specific embodiment two: the difference between this embodiment and specific embodiment one is that Bacillus subtilis is purchased from the China Agricultural Microorganism Culture Collection Center (ACCC) in step one, and the strain preservation number is 01185, and the preservation date is February 25, 2002 day. Other steps and parameters are the same as those in Embodiment 1.
specific Embodiment approach 3
[0037] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the Streptococcus thermophilus in step 1 was purchased from the China Agricultural Microorganism Culture Collection Center (ACCC), the strain preservation number is 10213, and the preservation date is 2006 June 20. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.
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