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Nisin/chitosan nano particle antibacterial film, preparation method and application

A nisin and chitosan nanotechnology, applied in the field of food antibacterial and fresh-keeping, can solve the problems of aggregation and decline, and reduce the biological activity of encapsulated substances, so as to prolong the shelf life, inhibit the growth of pathogenic microorganisms, and achieve biocompatibility. Good results

Active Publication Date: 2017-07-21
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, when nanoparticles are sprayed directly on the surface of food, they are prone to aggregation and drop phenomenon, which reduces the biological activity of encapsulated substances

Method used

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  • Nisin/chitosan nano particle antibacterial film, preparation method and application
  • Nisin/chitosan nano particle antibacterial film, preparation method and application
  • Nisin/chitosan nano particle antibacterial film, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The characterization of embodiment 1 nisin / chitosan nanoparticles

[0031] 1 Experimental materials

[0032]

[0033] 2 Experimental methods

[0034] 1) Preparation and characterization of nisin / chitosan nanoparticles

[0035] ① Take 500 μL of acetic acid and add deionized water to prepare 50 mL of 1% (V / V) acetic acid solution, and stir magnetically for 20 minutes.

[0036] ② Weigh 200mg of chitosan and add it to the above acetic acid solution to prepare a 4mg / mL chitosan solution, stir for 30min, then absorb 25mL of chitosan solution and add 25mL of deionized water to dilute.

[0037] ③Weigh 50mg of γ-polyglutamic acid and add to 25mL deionized water, stir for 10min.

[0038] ④ Weigh 500 mg of nisin and add it to 25 mL of deionized water, and stir for 15 min.

[0039] ⑤ Add nisin solution (20mg / mL) dropwise to γ-polyglutamic acid solution (2mg / mL) and stir for 30min.

[0040] ⑥ Add 50 mL of the above solution dropwise to 50 mL of chitosan solution (2 mg / mL), a...

Embodiment 2

[0052] The characterization of embodiment 2 nisin / chitosan nanoparticle antibacterial film

[0053] 1 Experimental materials

[0054] Polyethylene oxide SIGMA-ALDRICH

[0055] Embodiment 1 chitosan nanoparticle

[0056] 2 Experimental methods

[0057] 1) Preparation of nisin / chitosan nanoparticle antibacterial film

[0058] ①Dissolve the chitosan nanoparticles obtained by centrifugation in 99.75mL deionized water.

[0059] ② Weigh 600 mg of polyethylene oxide and slowly add it into 9.4 g of chitosan nanoparticle solution and stir overnight to prepare a 6 wt% (g / g) polyethylene oxide spinning precursor solution.

[0060] ③The syringe absorbs 5mL of the spinning precursor solution, the electrospinning voltage is 20kV, the propulsion rate is 0.6mL / h, the receiving distance is 15.5cm, the air humidity is controlled below 35%, and the nanofibers are obtained after 3 hours by using the flat plate collection method membrane.

[0061] ④According to the spinning step, without add...

Embodiment 3

[0066] The bacteriostatic zone experiment of embodiment 3 nisin / chitosan nanoparticle antibacterial film

[0067] 1 Experimental materials

[0068] Polyethylene oxide fiber film; nisin / chitosan nanoparticle antibacterial film

[0069] Listeria

[0070] 2 Experimental methods

[0071] 1) Prepare bacterial liquid

[0072] Inoculate Listeria in PYG liquid medium, place in an air shaker, and shake and culture at 37°C and 140rpm for 24 hours to obtain a bacterial suspension in the logarithmic growth phase. After mixing with a shaker, dilute 10000 times (approximately 10 4 cfu / mL).

[0073] 2) Electrospinning fiber membrane treatment

[0074] Prepare nisin / chitosan nanoparticle antibacterial film and polyethylene oxide fiber film according to Examples 1 and 2, and dry in an oven for 4 hours. The film was cut into a size of 1 cm×1 cm and placed under ultraviolet light for sterilizing treatment for 1 h for later use.

[0075] 3) Inhibition zone experiment

[0076] The sterili...

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Abstract

Belonging to the field of antibacterial preservatives and food fresh-keeping packaging materials, the invention in particular relates to a nisin / chitosan nano particle antibacterial film, a preparation method and application. Nisin crosslinks with gamma-polyglutamic acid, then chitosan is added to undergo crosslinking with gamma-polyglutamic acid so as to prepare nisin loaded gamma-polyglutamic acid / chitosan nano particles, thus improving the defect of bioactivity decrease of nisin during use and enhancing the utilization rate, then the nisin loaded chitosan nano particles are prepared into an antibacterial film by means of electrospinning so as to reduce the aggregation and drop phenomena of chitosan nano particles during direct sprinkling to food surfaces and avoid bioactivity decrease of encapsulated substances, thereby reaching the purposes of long-acting antibacterial properties and efficient utilization. According to the invention, the operation is simple, by applying the antibacterial film to fresh-keeping of cheese products, growth of pathogenic microorganisms can be effectively inhibited, and the shelf life of products can be prolonged.

Description

technical field [0001] The invention belongs to the field of antibacterial and fresh-keeping food, and specifically relates to a nisin / chitosan nanoparticle antibacterial film, a preparation method and application. Background technique [0002] Nisin is a polypeptide substance produced by Streptococcus lactis. Nisin can effectively inhibit the growth and reproduction of many Gram-positive bacteria that cause food spoilage, such as Staphylococcus aureus and Listeria monocytogenes, and is a food preservative certified by GRAS. It will be hydrolyzed into amino acids soon after eating, and will not change the normal flora in the human intestinal tract and produce resistance problems like other antibiotics. It is a natural food preservative with high efficiency, non-toxicity, safety and no side effects. It has been widely used in dairy products, canned products, fish products and alcoholic beverages. [0003] However, when nisin is directly used in food, its biological activity...

Claims

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Application Information

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IPC IPC(8): A23C19/097D04H1/728D01D5/00D01F6/94D01F1/10
CPCA23C19/097D01D5/0015D01F1/10D01F1/103D01F6/94D04H1/728
Inventor 林琳柏梅崔海英
Owner JIANGSU UNIV
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