Application of luteolin in bacterial quorum sensing inhibition system

A technology of luteolin and hemolysin, which is applied in the field of medicine to achieve the effect of reducing drug resistance and outcome

Active Publication Date: 2015-12-16
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, we found that luteolin has the effect of inhibiting the formation of bacterial biofilm, so it is expected to be used as a specific inhibitor of biofilm formation, which has not been reported yet

Method used

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  • Application of luteolin in bacterial quorum sensing inhibition system
  • Application of luteolin in bacterial quorum sensing inhibition system
  • Application of luteolin in bacterial quorum sensing inhibition system

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0041] Determination of minimum inhibitory concentration (MIC) of luteolin against Staphylococcus aureus

[0042] Take luteolin with a concentration of 512 μg / mL for doubling dilution, and then add it to a 96-well round-bottom culture plate, so that the concentration of Staphylococcus aureus (S.aureusN315) in the plate is OD 600 = 0.05. Place the 96-well plate at 37°C for 24 hours, then take the bacterial solution from each well, inoculate it on LB solid medium, and continue to cultivate at 37°C for 24 hours. The minimum concentration of the drug that is observed without bacterial growth is the minimum concentration of the drug. Inhibitory concentration.

[0043] The results showed that the minimum inhibitory concentration of luteolin on S.aureusN315 was 128 μg / mL (the minimum inhibitory concentration of vancomycin on S.aureusN315 was only <1 μg / mL). It can be seen that its effect is weak, and its prospects as an inhibitor of the growth and reproduction of Staphylococcus aur...

example 2

[0045] Determination of the concentration of luteolin Staphylococcus aureus on biofilm

[0046]Biofilm formation ability was analyzed by crystal violet staining in 96-well plate. The blood agar plate was transferred to S.aureusN315 for overnight activation, and a single colony was picked and shaken in 10mL TSB broth for 24 hours. The turbidity of the bacterial solution was diluted to 0.5 McFarland units, and then diluted 100 times with TSB broth. The experiment was divided into a blank control group and a luteolin treatment group (final concentrations were 8, 16, 32, 64 μg / mL, respectively). Three replicate wells were set up in each group and incubated at 37°C for 24 hours. Gently wash twice with 200 μL PBS buffer per well, and place the 96-well plate upside down in a ventilated and cool place for air-drying and fixing. After fixing for 24 hours, stain with 200 μL of 1% crystal violet solution per well for 10 minutes, remove the crystal violet solution, and rinse the 96-well ...

example 3

[0049] Observation of Biofilm Inhibitory Effect of Luteolin on Staphylococcus Aureus

[0050] Use TSB medium (tryptone soybean broth medium) containing 0.25% glucose to prepare a final concentration of 1 × 10 per milliliter 5 For each working bacterial solution, add 200 μL of working bacterial solution to each well of a 96-well plate; first add a pre-soaked cover glass to a 6-well plate, and then add 3 mL of working bacterial solution. The culture plate added with the bacterial solution was placed in a 37°C incubator for 24h. Biofilms were formed on coverslips in 96-well plates and 6-well plates after incubation. They were stained with 0.5% crystal violet staining solution, 5% silver nitrate solution and fluorescent dyes (SYTO9 and PI), and observed with fluorescence microscope and laser confocal microscope, respectively.

[0051] The results showed that both 1 / 8MIC (16μg / ml) and 1 / 16MIC (8μg / ml) luteolin could significantly inhibit the biofilm formation of S.aureusN315. se...

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Abstract

The invention relates to the technical field of medicine, and provides application of luteolin in a bacterial quorum sensing inhibition system, application of luteolin in preparing drug for lowering pathogenicity of staphylococcus aureus virulence factors and application of luteolin in preparing drug for inhibiting transcription of hla and agrA genes of staphylococcus aureus. Minimum effective concentration with application value is provided for the application of luteolin in the bacterial quorum sensing inhibition system, and a reference is provided for medically preparing specific bio-film forming inhibitors. The invention further provides a drug combination for inhibiting formation of staphylococcus aureus bio-films. The drug combination contains luteolin. According to the drug combination, formation of the bio-films of staphylococcus aureus can be inhibited by effectively inhibiting a quorum sensing system of staphylococcus aureus through tiny luteolin, prognosis risk, of staphylococcus aureus, for infection with high-drug-tolerance bio-films in anti-infection treatment is lowered, and a valuable reference is provided for medically preparing specific preparations for inhibiting the staphylococcus aureus bio-films.

Description

technical field [0001] The invention relates to the technical field of medicine. More specifically, the present invention relates to the use of luteolin in inhibiting bacterial quorum sensing systems. Background technique [0002] Bacterial biofilm is a bacterial community with a special microenvironment formed when bacteria are subjected to environmental stress (such as antibiotics), which can adhere to tissue cells or implanted devices, forming clinically difficult to cure At the same time, bacteria in the biofilm are 10 to 1000 times more resistant to antibiotics than those in the planktonic state. Biofilm is the main cause of chronic infection and infection recurrence, and causes the disease to persist; it can also cause the spread of bacteria between the environment and the host, and the spread and deterioration of the infection in the host. The formation of bacterial biofilm often occurs in clinical anti-infection treatment, and the external pressure exerted by antib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K36/704A61P31/04
Inventor 枉前
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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