Ethanol extract of radix tetrastigmae leaves as well as preparation method and application of ethanol extract

A technology of ethanol extraction and clover, applied in botany equipment and methods, applications, food preparation, etc., to achieve broad application prospects, inhibit bacterial growth, and inhibit the effect of killing bacteria

Inactive Publication Date: 2015-11-04
ZHEJIANG ESSENCE FUNGI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to extract the best antibacterial clover stock solution through the extract is not disclosed in the patent application specification.

Method used

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  • Ethanol extract of radix tetrastigmae leaves as well as preparation method and application of ethanol extract
  • Ethanol extract of radix tetrastigmae leaves as well as preparation method and application of ethanol extract
  • Ethanol extract of radix tetrastigmae leaves as well as preparation method and application of ethanol extract

Examples

Experimental program
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Effect test

Embodiment 1

[0022] (1) Get dried leaves of Cloverleaf and pulverize them to obtain dry powder, extract with 95% ethanol aqueous solution for 90min, the ratio of the quality of dry powder to the volume of ethanol aqueous solution is 1g:15mL, filter, and dry the filtrate to obtain Cloverleaf ethanol extract of

[0023] (2) The ethanol extract of Clover clover is dissolved in water containing DMSO (dimethyl sulfoxide) of 2% by volume at a concentration of 16 mg / mL, and is filtered and sterilized through a 0.45 μm filter membrane to obtain an antibacterial The medicinal solution, each medicinal solution was stored at 4°C for standby use.

[0024] (3) Detect the minimum inhibitory concentration (MIC) of each liquid medicine on bacteria. That is, bacteria were cultured overnight at 37°C in LB medium, then transferred to new LB medium at 1:15 and continued to grow to OD 620 =0.2±0.02. Dilute the bacteria into LB medium at 1:10000, add an equal volume of drug solution, mix well, take the same ...

Embodiment 2

[0030] (1) Get dried leaves of A. trifoliata and pulverize to obtain dry powder, extract with 70% ethanol aqueous solution for 90min, the ratio of the quality of dry powder to the volume of ethanol aqueous solution is 1g:15mL, filter, and dry the filtrate to obtain Aolys clover ethanol extract of

[0031] (2) The ethanol extract of Clover leaf is dissolved in the water containing 2% ethanol by volume percentage with the concentration of 16mg / mL, after the filter membrane of 0.45 μm is filtered and sterilized, antibacterial medicinal liquid is obtained, and each medicinal liquid is at 4 ℃ Conditions spare.

[0032] (3) Detect the minimum inhibitory concentration (MIC) of each liquid medicine on bacteria. That is, bacteria were cultured overnight at 37°C in LB medium, then transferred to new LB medium at 1:15 and continued to grow to OD 620 =0.2±0.02. Dilute the bacteria into LB medium at 1:10000, add an equal volume of drug solution, mix well, take the same volume of the mix...

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Abstract

The invention discloses an ethanol extract of radix tetrastigmae leaves as well as a preparation method and application of the ethanol extract. The preparation method comprises the steps of smashing dried radix tetrastigmae leaves to obtain dry powder; extracting by using an ethanol water solution, and filtering to obtain filtrate; and drying the filtrate to obtain the ethanol extract of the radix tetrastigmae leaves. According to the preparation method, bacterium inhibiting and killing components in the radix tetrastigmae leaves are extracted through extracting by using the ethanol water solution, and finally, the ethanol extract of the radix tetrastigmae leaves is obtained. The ethanol extract of the radix tetrastigmae leaves, prepared by using the preparation method, can be used for preparing bacterium inhibiting and killing supplies, can be used for inhibiting and killing bacteria on drugs, cosmetics, health foods and foods as well as bacteria generated in human, animals and aquaculture, and can be used for effectively inhibiting bacterial growth and killing bacteria.

Description

technical field [0001] The invention relates to the technical field of the extract of A. trifoliata, in particular to an ethanol extract of A. clover and its preparation method and application. Background technique [0002] The traditional Chinese medicine clover (Tetrastigma hemsleyanum Diels et Gilg) is a vine of the family Vitis genus. It grows in damp hillsides, valleys or under forests beside streams. It is mainly distributed in southwest my country, Zhejiang, Anhui and Jiangxi. , Fujian, Guangxi and other provinces. Clover is mainly used as root, and the whole plant is also used as medicine, but there is no special description. At present, Clover sold in pharmacies and commercially available Chinese herbal medicine powder are all from the root of Clover. Qing also mostly refers to the root part of Clover. The roots of Clover have the effects of improving immunity, anti-virus, and enhancing liver function, especially for hepatitis B, various tumors, cancer, febrile con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/87A61K8/97A23L1/29A61P31/04A01N65/08A01P1/00A23L33/00
Inventor 吴学谦俞盈苏庆亮熊科辉许海顺徐娟吴学良楼利明
Owner ZHEJIANG ESSENCE FUNGI DEV
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