Tetrastlgma hemsleyanum vine polysaccharide extract, preparation method and application thereof

A cloverleaf, extract technology, applied in application, food preparation, food science and other directions, to achieve the effect of broad application prospects

Active Publication Date: 2015-11-18
ZHEJIANG ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how to extract the best antibacterial clover stock solution thro

Method used

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  • Tetrastlgma hemsleyanum vine polysaccharide extract, preparation method and application thereof
  • Tetrastlgma hemsleyanum vine polysaccharide extract, preparation method and application thereof
  • Tetrastlgma hemsleyanum vine polysaccharide extract, preparation method and application thereof

Examples

Experimental program
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Example Embodiment

[0030] Example 1

[0031] (1) Take 50g of the dried vine of A. clover and pulverize it to obtain dry powder, use 250mL of water as a solvent to extract 90min, filter, and dry the filtrate to obtain the water extract of A. clover; the water extract of A. clover is dissolved in 5 mL of water, and add 90% by volume ethanol aqueous solution was precipitated at 4° C. for 12 hours, and the precipitate was dried to obtain a crude polysaccharide extract of Ivy clover. Add the crude polysaccharide extract 50mg of Ivy clover to 5mL water, ionic liquid [C 4 min] Cl10mL, K 3 PO 4 1g, stand at 35°C for 20min, centrifuge at 3000rpm for 10min, and separate into upper and lower phases. Remove the phase liquid, and use 5 times the volume of 90% ethanol aqueous solution to precipitate at 4° C. for 12 hours to obtain an extract of Ivy clover polysaccharides. The content of Ivy clover polysaccharides is 87.7% as measured by the phenol-sulfuric acid method. The polysaccharide extract of Ivy cl...

Example Embodiment

[0037] Example 2

[0038] (1) Take 50g of dried vines of A. clover and pulverize to obtain dry powder, use 2L of water as a solvent to extract 90min, filter, and dry the filtrate to obtain the water extract of A. clover; the water extract of A. clover is dissolved in 10 mL of water, and 40 mL of The crude polysaccharide extract of Ivy clover was obtained by precipitation in 80% ethanol aqueous solution at 4° C. for 12 h. Add 10mg of the crude polysaccharide extract of Ivy clover to 5mL water, ionic liquid [C 4 min] Cl 1mL, K 2 HPO 4 1g, stand at 20°C for 60min, centrifuge at 5000rpm for 20min, and separate into upper and lower phases. Remove the phase liquid, and use 4 times the volume of 80% ethanol aqueous solution to precipitate at 4°C for 12 hours to obtain the polysaccharide extract of Ivy clover. The content of the polysaccharide of Ivy clover is 92.3% as measured by the phenol-sulfuric acid method. The polysaccharide extract of Ivy clover was dissolved in water with...

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Abstract

The invention discloses a tetrastlgma hemsleyanum vine polysaccharide extract, a preparation method and application thereof. The method includes: crushing dry tetrastlgma hemsleyanum vine to obtain dry powder, conducting extraction with water, performing filtering, and drying the filtrate to obtain a tetrastlgma hemsleyanum vine aqueous extract, and subjecting the tetrastlgma hemsleyanum vine aqueous extract to ethanol precipitation and two-aqueous phase extraction, thus obtaining the tetrastlgma hemsleyanum vine polysaccharide extract with a polysaccharide content of 53.7%-97.8%. The tetrastlgma hemsleyanum vine polysaccharide extract prepared by the method provided by the invention has antibacterial and bactericidal effects, and can be used for preparation of bacteriostatic and bactericidal articles. If the tetrastlgma hemsleyanum vine polysaccharide extract is prepared into an antibacterial liquid medicine, the medicine liquid can be used as a component in drugs, cosmetics, health food, food and used for bacteriostasis and antibiosis in people, animals and aquaculture process. Besides the original public efficacy of tetrastlgma hemsleyanum, the tetrastlgma hemsleyanum vine polysaccharide extract has new use in drugs, cosmetics, health food and food, and has bacteriostatic and bactericidal effects, thus having broad application prospects.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extract from Ivy clover, in particular to a polysaccharide extract from Ivy clover and its preparation method and application. Background technique [0002] The traditional Chinese medicine clover (Tetrastigmahemsleyanum Dielset Gilg) is a vine of the family Vitis genus. It grows in damp hillsides, valleys or under forests beside streams. It is mainly distributed in southwest my country and Zhejiang, Anhui, Jiangxi, Fujian and Guangxi. And other provinces. Clover is mainly used as root, and the whole plant is also used as medicine, but there is no special description. At present, Clover sold in pharmacies and commercially available Chinese herbal medicine powder are all from the root of Clover. Qing also mostly refers to the root part of Clover. The roots of Clover have the effects of improving immunity, anti-virus, and enhancing liver function, especially for hepatitis B, various tumors, ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P31/04A61K8/73A61Q19/00A23L1/09
Inventor 俞盈吴学谦许海顺熊科辉么春艳徐娟
Owner ZHEJIANG ACAD OF MEDICAL SCI
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