The invention relates to culture medium for fermentation production of erythromycin with red streptomyces and a fermentation method thereof. The culture medium includes seed culture medium and fermentation culture medium, wherein the seed culture medium is prepared from starch, corn steep liquor, ammonium sulfate, light calcium carbonate, zinc sulfate, magnesium sulfate and a right amount of defoaming agent, and the fermentation culture medium is prepared from starch, corn steep liquor, ammonium sulfate, calcium carbonate, magnesium sulfate, zinc sulfate and a right amount of defoaming agent.According to the culture medium and the fermentation method, through change of the formula structures of the seed culture medium and the fermentation culture medium, change of the formula structure and control means of additional medium and introduction of several trace elements, the metabolic pathway of cells is changed, so that tertiary fermentation is shortened to secondary fermentation, and the fermentation time is shortened; meanwhile, the cost of fermentation raw materials is reduced, the fermentation culture medium is diluted, oxygen supply in the fermentation process is improved, and energy consumption of oxygen supply is reduced; through implementation of the technology, the fermentation level of erythromycin is significantly improved, and erythromycin E in the product is significantly reduced.