Red Saccharopolyspora and method for producing erythromycin by fermenting Saccharopolyspora

Abstract

The invention relates to Saccharopolyspora and a method for producing erythromycin by fermenting the Saccharopolyspora. The depositary institution of Saccharopolyspora erythraea HD03 is the common microbe center of China microbial culture preservation commission; the preservation code is GMCC No.2916; the method comprises the following specific steps that: a block with area of 1 cm*1 cm or a glycerin tube is dug from the preservation bevel and is inoculated into a sterilized seed culture medium; the temperature is between 32 and 34 DEG C; the rotational speed of a rocking bed is between 220 and 240 revolutions/minute; the time for cultivation is between 44 and 48 hours; subsequently, the block or the glycerin with 3 to 10 percent of inoculation amount is inoculated in a sterilized fermentation medium; the temperature is between 32 and 34 DEG C; the rotational speed of the rocking bed is between 220 and 240 revolutions/minute; the time for cultivation is 6 to 7 days; n-propyl alcohol as a precursor is added into the fermentation medium at 20 to 24 hours; and at late stage of fermentation, a methylation reagent and a hydroxylation reagent are added into the fermentation medium to obtain the erythromycin. The method has the advantages that: in the fermentation process, the addition of the methylation reagent and the hydroxylation reagent improves the methylation action and the hydroxylation action of the precursor in the process of synthesizing the erythromycin, reduces the accumulation of byproducts and improves the yield of main products.

Description

【Technical field】 [0001] The invention relates to the technical field of erythramycin, in particular to a red Saccharopolyspora erythraea HD03 CGMCC No.2916 and a method for producing erythramycin by fermentation thereof. 【Background technique】 [0002] Erythromycin (Er) is a secondary metabolite synthesized by Saccharopolysporaerythraea and is a 14-membered macrolide antibiotic. Erythromycin has a broad-spectrum antibacterial effect, and has antibacterial activity against Gram-positive bacteria, acid-fast bacilli, Rickettsia and large viruses. The drug of choice for the disease, clinically available for patients allergic to penicillin. Since the 1990s, erythromycin has been selling well in the international market, and its output has increased significantly, becoming the third largest class of drugs in the world antibiotic market. [0003] Erythromycin is a multicomponent antibiotic. Erythromycin A (ErA), which is widely used clinically, has the highest antibacterial act...

AI Technical Summary

Problems solved by technology

However, although high dissolved oxygen concentration is necessary, high intracellular aglycone flux can also lead to the conversion of ErD intermediates to ErB, a side product.