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Gastrodia elata macromolecular linear straight-chain glucan and preparation method and application thereof

A glucan and macromolecular technology, applied in the field of traditional Chinese pharmacy, can solve the problems such as the lack of large-molecular-weight gastrodia elata linear glucan, affecting the separation and extraction process, difficulty in development and utilization, etc. The effect of macrophage proliferation

Active Publication Date: 2021-03-30
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The Gastrodia elata polysaccharides extracted in the above studies are all composed of glucose and other monosaccharides. They are heteropolysaccharides with complex structures, which brings certain difficulties to their development and utilization, and their molecular weights are small, which affects their separation and extraction process. Makes it easy to appear impurities during separation
There is no report of large molecular weight Gastrodia elata linear dextran

Method used

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  • Gastrodia elata macromolecular linear straight-chain glucan and preparation method and application thereof
  • Gastrodia elata macromolecular linear straight-chain glucan and preparation method and application thereof
  • Gastrodia elata macromolecular linear straight-chain glucan and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Extraction of Gastrodia elata α-D-1,4-glucan

[0041] A. Dry and pulverize the tubers of Gastrodia elata. The obtained powder is degreased with 80% ethanol for 24 hours. After drying at room temperature, 100 g of Gastrodia elata powder is added to 3000 mL of distilled water, soaked at room temperature for 2 hours, stirred and extracted for 3 hours, centrifuged, and the residue is extracted twice and combined. The supernatant was then concentrated, and 3 times the volume of 95% ethanol was added for precipitation, and the precipitate was freeze-dried to obtain the crude polysaccharide of Gastrodia elata cold water.

[0042] B. Take Gastrodia elata crude polysaccharide and prepare a 1% solution, add an equal volume of dichloromethane / n-butanol mixture (chloroform:n-butanol=4:1, volume ratio), stir to remove protein, repeat the deproteinization twice , the polysaccharide solution after protein removal was concentrated, and dialyzed with a dialysis bag with a molecular w...

Embodiment 2

[0044] 1. Extraction of Gastrodia elata α-D-1,4-glucan

[0045] A. Dry and pulverize the tubers of Gastrodia elata. The obtained powder is degreased with 80% ethanol for 24 hours. After drying at room temperature, 100 g of Gastrodia elata powder is added to 2000 mL of distilled water, soaked at room temperature for 1 hour, stirred and extracted for 3 hours, centrifuged, and the residue is repeatedly extracted for 3 times and combined. The supernatant was concentrated, and 2 times the volume of 95% ethanol was added for precipitation, and the precipitate was freeze-dried to obtain the crude polysaccharide of Gastrodia elata cold water.

[0046] B. Take Gastrodia elata crude polysaccharide and prepare a 1% solution, add an equal volume of dichloromethane / n-butanol mixture (chloroform:n-butanol=4:1, volume ratio), stir to remove protein, repeat the deproteinization twice , the polysaccharide solution after protein removal was concentrated, and dialyzed with a dialysis bag with a ...

Embodiment 3

[0048] 1. Extraction of Gastrodia elata α-D-1,4-glucan

[0049] A. Dry and pulverize the tubers of Gastrodia elata. The obtained powder is degreased with 80% ethanol for 24 hours. After drying at room temperature, 100 g of Gastrodia elata powder is added into 6000 mL of distilled water, soaked at room temperature for 6 hours, stirred and extracted for 3 hours, centrifuged, and the residue is extracted three times and combined. The supernatant was concentrated, and 6 times the volume of 95% ethanol was added for precipitation, and the precipitate was freeze-dried to obtain the crude polysaccharide of Gastrodia elata cold water.

[0050] B. Take Gastrodia elata crude polysaccharide and prepare a 1% solution, add an equal volume of dichloromethane / n-butanol mixture (chloroform:n-butanol=4:1, volume ratio), stir to remove protein, repeat the deproteinization twice , the polysaccharide solution after protein removal was concentrated, and dialyzed with a dialysis bag with a molecula...

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Abstract

The invention discloses gastrodia elata macromolecule linear straight-chain glucan and a preparation method and application thereof, and belongs to the field of traditional Chinese medicine. The method comprises the steps: drying gastrodia elata tubers, smashing and degreasing with ethyl alcohol; extracting at room temperature, centrifuging and concentrating an extracting solution, carrying out alcohol precipitation, precipitating and redissolving, dialyzing by adopting a dialysis bag with the molecular weight cutoff of 8000-14000 Da, concentrating the dialyzed solution, and further separatingand purifying by using ion exchange resin and gel exclusion chromatography to obtain the gastrodia elata macromolecular linear straight-chain glucan. The polysaccharide is only composed of glucose, is alpha-D-1, 4 connected homopolydextran, and is a new dextran. When the concentration is 400 [mu] g / mL, macrophage proliferation can be significantly promoted, the phagocytic ability of macrophages can be enhanced, the macrophages can be promoted to release NO and cytokines TNF-alpha and IL-6, and the body immunity can be enhanced.

Description

technical field [0001] The invention relates to the field of traditional Chinese pharmacy, in particular to a linear linear glucan of Gastrodia elata macromolecules and a preparation method and application thereof. Background technique [0002] Gastrodia elata Blume (Gastrodia elata Blume) is a heterotrophic perennial herb of the genus Gastrodia of Orchidaceae. Its dried tubers for medicinal purposes are mainly produced in the four provinces of Yunnan, Guizhou, Sichuan and Tibet in my country. Gastrodia elata is mainly used for the treatment of hypertension, dizziness, headache, deviated mouth and eyes, limb numbness, infantile convulsions, epilepsy convulsions, and tetanus. Modern pharmacological studies have found that Gastrodia elata also has the functions of increasing intelligence and strengthening the brain, scavenging free radicals, and delaying aging, and has a certain curative effect on senile dementia. Polysaccharide is an important constituent substance in Gastr...

Claims

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Application Information

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IPC IPC(8): C08B37/02A61K31/716A61P37/04A23L33/125
CPCC08B37/0009C08B37/0003A61K31/716A61P37/04A23L33/125A23V2002/00A23V2250/5042A23V2200/324
Inventor 李红燕解万翠杨锡洪宋琳车红霞董秀芳
Owner QINGDAO UNIV OF SCI & TECH
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