28 results about "Gene and protein expression" patented technology

A spatial omics multi-modal fusion method under a single cell level comprises the following steps: extracting spatial morphological characteristics of differential expression genes and cell nucleuses from spatial transcriptome data, single cell sequencing data and histological images, and realizing field adaptation among different platforms by using a conditional variation auto-encoder. And based on a probability inference model, fusing spatial transcriptome expression, unicellular omics and morphological characteristics, and jointly inferring the type and gene expression level of each cell. A spatial cell network is constructed through a graph attention mechanism, and spatial diffusion and recognition of cell types in a full slice range are realized. In combination with a multi-omics enhancement module, undetected gene and protein expression is completed based on expression similarity, and prediction consistency is improved through spatial correction. According to the method, high-resolution reconstruction of single-cell multi-omics information in a three-dimensional space is realized, the information coverage and spatial resolution of spatial omics data are improved, and an efficient and low-cost solution is provided for spatial biology and precise medical research.

The invention discloses Dong'e donkey-hide gelatin active peptide and application thereof. Relates to the technical field of biology. The invention provides an amino acid sequence and application of the Dong'e donkey-hide gelatin active peptide. The amino acid sequence of the CCA active peptide is YYTSASGDEMVSLK, and the CCA active peptide can significantly up-regulate the I-type collagen gene expression, increase the I-type and III-type collagen content and reduce senescence markers. The peptide fragment can be combined with TGFBR2, especially TGFBR1, gene and protein expression of the TGFBR2 can be up-regulated, downstream targets of a TGF-beta-SMAD pathway can be activated, and the MMPs / TIMPs ratio can be reduced. According to the present invention, the TGF beta-SMAD pathway is activated, the MMPs / TIMPs ratio is reduced, the collagen synthesis is promoted, and the excessive decomposition of the collagen is inhibited, such that the I type collagen content and the III type collagen content are increased, and the aging degree of the photoaging dermal fibroblast is reduced;

The invention discloses a virus infection risk assessment method based on procambarus clarkia TRI M23 analysis, which comprises the following steps: acquiring TRI M23 gene and protein expression data of procambarus clarkia in a culture cycle, performing correlation analysis through an expression sequence and a contrast sequence, assessing infection probability, and judging infection aggregation of each region based on a Moran's I index. The infection migration and the infection risk of each region are predicted, and meanwhile, a monitoring strategy and an isolation scheme are dynamically set. The method solves the problems that a traditional method depends on experience evaluation and risk analysis is high in hysteresis quality, the biological detection technology is combined, the risk prediction accuracy is remarkably improved, and a scientific basis is provided for aquaculture virus prevention and control.

The invention relates to the field of biological medicines, and discloses application of a GOSR1 gene in prevention and / or treatment of respiratory pathogens. The GOSR1 gene is specifically knocked down, so that the gene and protein expression level of the pathogen can be reduced, and the proliferation of the respiratory pathogen can be remarkably inhibited. In addition, the invention also discloses that overexpression of the GOSR1 gene can activate proliferation of pathogens in cells infected by respiratory pathogens. Based on the regulation effect of the GOSR1 gene on respiratory tract pathogen proliferation, a brand new strategy is provided for treatment of respiratory tract pathogen infection, and the application is particularly suitable for intervention of influenza A virus infection.

The invention discloses application of mitoxantrone in preparation of an anti-rabies virus drug, reveals that clinical anti-tumor and immunosuppression drug mitoxantrone has remarkable anti-rabies virus activity for the first time, and opens up a new application field for mitoxantrone. The rigorous in-vitro and in-vivo tests prove that the mitoxantrone can inhibit virus replication in a dose-dependent manner, reduce virus gene and protein expression and remarkably improve the survival rate of infected mice in an animal model. The invention provides a new clinical application approach for mitoxantrone, provides a powerful candidate drug and a brand-new technical scheme for anti-rabies virus drugs, and has important practical significance and clinical application prospects for coping with rabies which is a major public health threat.

The application provides application of ABT199 in preparation of a drug for treating muscle atrophy. Compared with the prior art, the application provides a new use of ABT199 in treating muscle atrophy. Experimental research proves that ABT199 administration can significantly restore the exercise capacity of a cachexia-induced muscle atrophy mouse, improve the muscle cross-sectional area of the mouse, restore the reduced glutathione content of the mouse, and improve the superoxide dismutase activity, so as to improve the muscle atrophy phenotype of the mouse and muscle atrophy-related biochemical indexes. ABT199 can significantly restore cell muscle atrophy-related gene and protein expression, and improve the cachexia-induced muscle atrophy phenotype by reducing related inflammatory mediators; ABT199 can significantly improve the mitochondrial membrane potential, increase the expression of mitochondrial neogenesis-related genes, and reduce the production of active oxygen.

A single-cell level spatial omics multi-modal fusion method, comprising: extracting differential expression genes and nuclear spatial morphological features from spatial transcriptome data, single-cell sequencing data and histological images, and realizing field adaptation between different platforms by using conditional variational autoencoder. Based on a probability inference model, spatial transcriptome expression, single-cell omics and morphological features are fused to jointly infer the type and gene expression level of each cell. A spatial cell network is constructed by a graph attention mechanism to realize the spatial diffusion and recognition of cell types in the whole slice range. Combined with a multi-omics enhancement module, the unmeasured gene and protein expression are completed based on expression similarity, and the prediction consistency is improved through spatial correction. The method realizes high-resolution reconstruction of single-cell multi-omics information in three-dimensional space, improves the information coverage and spatial resolution of spatial omics data, and provides an efficient and low-cost solution for spatial biology and precision medicine research.

The invention discloses ulva pertusa polysaccharide UPP and ulva pertusa homogeneous polysaccharide UPP-5 as well as a preparation method and application thereof. The single-component polysaccharide UPP-5 is obtained by adopting DEAE-52 anion exchange chromatography in combination with ultrafiltration purification. The structure of the UPP-5 is systematically characterized by high performance gel permeation chromatography, infrared spectroscopy, methylation analysis, nuclear magnetic resonance and other methods, and the result shows that the UPP-5 is acidic heteropolysaccharide with a novel structure. Based on an AOM / DSS induced colorectal cancer mouse model, the effect of the mouse model is evaluated from the aspects of disease activity index, colon length, tumor number, histological observation, gene and protein expression and the like. Results show that the UPP can significantly improve inflammation and pathological states and reduce the incidence of tumors, and has the potential of preventing and treating colorectal cancer. The invention provides a new thought and theoretical basis for structural research and medical function development of algal polysaccharides.

The invention provides application of an OMA1 inhibitor in preparation of a medicine for treating heart failure, relates to the technical field of biomedicine, and is technically characterized in that the OMA1 inhibitor is provided, preferably ZINC14880369, and the effect of the Oma1 small-molecule inhibitor ZINC14880369 in the heart failure is verified by adopting a heart failure model in an experiment. The heart failure model is constructed through aortic arch constriction. In-vitro experiments in the application verify that Oma1mRNA and protein expression in the heart of a mouse after heart failure are increased, the small-molecule inhibitor ZINC14880369 is designed and proved to be capable of effectively inhibiting Oma1 gene expression, and further research is carried out, so that a potential treatment strategy is provided for drug research and development. The Oma1 small-molecule inhibitor in the heart can relieve the heart failure course of a mouse and lower the expression of Oma1 mRNA at the same time. Therefore, down-regulation or inhibition of Oma1 gene and protein expression by using a small-molecule inhibitor is an effective way for treating heart failure.

The present invention relates to, among other things, probes, compositions, methods, and kits for simultaneous, multiplexed detection and quantification of protein and / or nucleic acid expression in a user-defined region of a tissue, user-defined cell, and / or user-defined subcellular structure within a cell that are adaptable for use with existing sequencing technologies.

Described herein are compositions and methods for accelerating the healing of a bone fracture in a subject, regenerating bone in a subject, and reducing the likelihood of bone loss in an osteoporotic environment of a subject. In some embodiments, the compositions and methods may comprise one or more inhibitors of p21 to suppress p21 gene and protein expression in a subject. In some embodiments, suppression of p21 expression in mesenchymal stem cells (MSCs) in a subject may reduce adipogenic differentiation, increase osteogenic capacity, and increase chondrogenic capacity in the subject.

The invention discloses a skin-derived staphylococcus coriolis CCSM006 for improving skin health based on multiple targets and a metagen thereof, and belongs to the field of microbial technologies and medicines. The staphylococcus coriolis CCSM006 provided by the invention shows a remarkable barrier repair effect in the aspect of external use, and the remarkable barrier repair effect is specifically shown as follows: in an in-vitro experiment, the activity of damaged HaCaT cells can be promoted, the gene and protein expression levels of functional proteins and structural proteins after damage are improved, and meanwhile, the expression of sphingomyelinase 1 (SMPD1) and tissue kinin enzyme 7 (KLK7) is remarkably improved. Research finds that the strain regulates the expression of skin barrier related proteins and enzymes through an AHR-OVOL1 pathway. The staphylococcus coriolis metagen has a wide application prospect in the field of cosmetics or medicines.

The present invention provides methods of profiling gene and protein expression of a plurality of nuclei from a single cell type and comparing the profiles to determine variability among cell populations, samples from different subjects, and cells expressing a disease phenotype.

The invention discloses a composition helpful for controlling in-vivo fat and application thereof, and relates to the technical field of biology. The hesperidin composition is prepared from hesperidin and lactobacillus johnsonii (Lactobacillus johnsonii), and the hesperidin composition is prepared from the hesperidin and the lactobacillus johnsonii. Research finds that the composition can achieve synergistic up-regulation of gene and protein expression of adipose tissue PGC1alpha and UCP1 and increase the body surface temperature; the composition can also significantly improve the bioavailability of hesperidin and improve the concentration of hesperetin and other metabolites in serum; the composition can also optimize the intestinal microecology, improve the intestinal flora structure, increase the lactobacillus colonization rate, enrich the abundance of beneficial bacteria and promote the generation of short-chain fatty acids; the composition can also significantly improve lipid metabolism disorder of an organism, reduce fat accumulation, regulate adiponectin and leptin levels, control fat in the body and realize effective weight loss management.

The present disclosure provides methods and systems for mapping gene and protein expression in a cell (i.e., mapping gene and protein expression within the same cell simultaneously). The present disclosure also provides methods for diagnosing a disease or disorder (e.g., a neurological disorder such as Alzheimer's disease) in a subject. Methods of screening for a candidate agent capable of modulating gene and / or protein expression are also provided by the present disclosure. The present disclosure also provides methods for treating a disease or disorder, such as Alzheimer's disease, in a subject in need thereof. A plurality of oligonucleotide probes, which may be useful for performing the methods described herein, are also described by the present disclosure, as well as kits comprising any of the oligonucleotide probes described herein. Additionally, the present disclosure provides methods, apparatuses, and non-transitory computer-readable storage media for identifying spatial variations of cell types in at least one image.

The invention relates to the field of biological medicines, discloses application of a VTI1A gene, and particularly relates to application of the VTI1A gene in prevention and / or treatment of respiratory pathogens. The VTI1A gene is specifically knocked down, so that the gene and protein expression level of the pathogen can be reduced, and the proliferation of the respiratory pathogen can be remarkably inhibited. In addition, the invention also discloses that overexpression of the VTI1A gene can activate proliferation of pathogens in cells infected by respiratory pathogens. Based on the regulation and control effect of the VTI1A gene on respiratory tract pathogen proliferation, a brand new strategy is provided for treatment of respiratory tract pathogen infection, and the application is particularly suitable for intervention of influenza A virus infection.

The invention relates to an in-vitro simulation device, in particular to an in-vitro vascular perfusion device and method for simulating shearing force of vascular tissue, and the device comprises a peristaltic pump, a liquid storage container, a constant-temperature perfusion box and a constant-temperature assembly; the peristaltic pump, the liquid storage container and the constant-temperature perfusion box are sequentially connected through a circulating pipeline; the constant-temperature perfusion box comprises a bearing seat, an upper cover and a sealing gasket; the upper cover covers the bearing seat; the sealing gasket is arranged between the upper cover and the bearing seat; two ends of the in-vitro blood vessel are respectively connected with the circulating pipeline through perfusion needles; the constant-temperature assembly is used for maintaining the internal temperature of the constant-temperature perfusion box. Compared with the prior art, the method solves the problem that an accurate simulation result cannot be obtained due to the fact that the shearing force environment borne by endothelial cells cannot be reproduced when artificial materials are used for simulating blood vessels in the prior art. According to the scheme, the perfusion device for simulating the shearing force of the vascular tissue in vitro is constructed based on the in-vitro blood vessel, and the gene and protein expression regulation mechanism of endothelial cells under the stimulation of different shearing forces can be disclosed more accurately.

The invention provides an application of APOL1 in diagnosis of neonatal necrotizing enterocolitis. Research finds that compared with a control child patient, the APOL1 gene and protein expression level in NEC child patients are remarkably improved for the first time, ROC curve analysis results show that when APOL1 is used for NEC diagnosis, AUC is 0.86, and diagnosis accuracy is high. Therefore, the APOL1 can be used as a specific diagnostic marker of the NEC for assisting clinical diagnosis of the NEC. Furthermore, when the APOL1 is combined with C reactive protein, lymphocyte count (LYM) or hemoglobin level to be used for NEC diagnosis, AUC can be further improved to 0.91-0.94, and the diagnosis accuracy is further improved. The invention provides a novel specific biomarker for diagnosis of NEC, and has important clinical value for early diagnosis and early treatment of NEC.

The invention discloses a construction method of a 3D bio-printed fatty liver tissue, and relates to the technical field of fatty liver models. Comprising the following steps: (1) carrying out 3D biological printing on liver tissues; and (2) inducing fatty liver tissues. The artificial liver tissue disclosed by the invention can be uniformly and standardly produced in vitro through a 3D biological printing technology, so that high-throughput screening of drugs is realized. Specific genes and protein expression levels and functional phenotypes of mature hepatocytes are shown in vitro. The successfully induced fatty liver model shows disease characterization corresponding to physiological lesions and makes sensitive response to drugs.

The invention relates to the technical field of animal models, in particular to construction and application of a Stard6 gene knockout mouse animal model, and the Stard6 gene knockout mouse animal model is obtained by mating a Stard6-flox mouse with a Star8-Cre mouse. A Stard6 gene knockout mouse model is constructed, and it is found that gene level expression and protein level expression of a specific Stard6 gene knockout mouse in male germ cells are remarkably reduced, so that testis development and spermatogenesis of a male mouse of the Stard6 gene knockout mouse are abnormal. Therefore, the problems that a common animal model cannot target a target gene, interference of other factors exists, an animal model with whole body knockout is prone to embryo death and the like are solved.

The invention relates to the field of biological medicines, and discloses application of an STX8 gene, in particular to application of the STX8 gene in prevention and / or treatment of respiratory pathogens. The STX8 gene is specifically knocked down, so that the gene and protein expression level of the pathogen can be reduced, and the proliferation of the respiratory pathogen can be remarkably inhibited. In addition, the invention also discloses that overexpression of the STX8 gene can activate proliferation of pathogens in cells infected by respiratory pathogens. Based on the regulation and control effect of the STX8 gene on respiratory tract pathogen proliferation, a brand new strategy is provided for treatment of respiratory tract pathogen infection, and the STX8 gene is particularly suitable for intervention of influenza A virus infection.

The invention provides a pichia pastoris vector for integrating a gene to a TEF1 site, the vector comprises a prokaryotic replication element, a resistance gene expression cassette and a gene expression cassette, and the gene expression cassette is driven by a TEF1 promoter. The invention further provides a construction method of the pichia pastoris vector for integrating the genes to the TEF1 site, and directional cloning of different and even multiple target genes and protein expression and secretion in pichia pastoris are achieved.

A construction method and use of a gastruloid stem cell line. Gastruloid stem cells that can be passaged stably are established in this research. The cells maintain the pluripotency of stem cells to a certain extent, exhibit gene and protein expression of cells from three germ layers, endoderm, mesoderm, and ectoderm, during gastrulation, and have characteristics consistent with those of cells during gastrulation, so that key characteristics of cells during gastrulation can be well reproduced. A three-dimensional gastruloid model that can mimic gastrulation can be constructed in mice or in vitro by using the stem cell line, and key biological events can be partially reproduced, so that key characteristics of post-implantation embryos during gastrulation can be well reproduced. Through this model, an in vitro platform for screening drugs affecting early embryonic development can be established, providing a reference for clinical medication.

The invention discloses application of an ADAM8 gene and / or ADAM8 protein in preparation of a medicine for treating chronic sinusitis with nasal polyp and the medicine, and relates to the technical field of biological medicines. According to the application of the ADAM8 gene and / or the ADAM8 protein in preparation of a medicine for treating chronic sinusitis with nasal polyp, the ADAM8 gene and / or the ADAM8 protein are / is used as a treatment target. The research finds that the gene and protein expression level of ADAM8 are obviously up-regulated in ECRSwNP and NECRSwNP and are mainly distributed in inflammatory cells of epithelium and below the epithelium, which prompts that ADAM8 may be a key pathogenic factor in CRSwNP pathogenesis. By specifically inhibiting the activity of ADAM8, nasal polyp-like lesions can be remarkably relieved, infiltration of eosinophilic granulocytes, neutrophil granulocytes and other inflammatory cells can be reduced, key inflammatory signal pathways can be inhibited, collagen deposition and metal matrix protease expression can be reduced, then inflammatory response and a tissue remodeling process can be effectively relieved, and the application of ADAM8 in treatment of nasal polyp-like diseases can be promoted. The scientificity and clinical application prospect of the ADAM8 as a treatment target are verified.

The invention belongs to the technical field of agricultural biology, and particularly relates to an application of a biological product for inhibiting expression of an OsCYP74C1 gene and a protein thereof in improving rice insect pest resistance. The amino acid sequence of protein coded by the OsCYP74C1 gene is shown as SEQ ID NO.1. The OsCYP74C1 gene disclosed by the invention is related to the resistance of rice to insect pests: after the OsCYP74C1 gene is over-expressed, the resistance of the rice to brown planthopper and chilo suppressalis can be reduced; and after the OsCYP74C1 gene in the rice is silenced by using the biological product of the silent OsCYP74C1 gene, the resistance of the rice to brown planthopper and chilo suppressalis pests can be remarkably enhanced, and new insect-resistant varieties of the rice can be cultivated by using the biological product of the silent OsCYP74C1 gene, so that the yield of the rice is increased.

The invention is applicable to the technical field of biomedicine, relates to a construction method and application of a diuretic-resistant cell model, and establishes the diuretic-resistant cell model by using angiotensin II and aldosterone to jointly induce mouse renal manifold M-1 cells. The model is verified by detecting gene and protein expression levels of aquaporin 2, epithelial sodium channel alpha subunit and arginine vasopressin receptor 2. The method is simple to construct and good in repeatability, can truly reflect the pathological characteristics of diuretic resistance, can stably simulate the state of water-sodium retention caused by excessive activation of a renin-angiotensin-aldosterone system, can reproduce the core characteristic of insufficient curative effect of the diuretic, verifies the occurrence effect of the diuretic resistance, and has a good application prospect. And a stable and controllable in-vitro model is provided for researching a molecular mechanism of diuretic resistance and screening prevention and treatment drugs.

The application relates to the technical field of probiotics, and discloses probiotic protein powder for promoting sleep. A preparation method comprises the following steps: co-fermenting protein raw materials and GABA-producing Lactobacillus brevis starter, stopping fermentation when the final pH value of fermentation is controlled to be reduced to 4.5-5.5, collecting the fermentation product and drying the fermentation product to obtain the probiotic protein powder. Through regulation of the final pH value of fermentation, the application realizes accurate control of the fermentation degree, guarantees the GABA yield, and retains the structural integrity of 500-3000 Da active peptides generated by hydrolysis of the protein raw materials, so that the fermentation product contains high-content GABA and high-proportion active peptides, the two coexist, respectively activate GABA A receptors and up-regulate GABA A receptor genes and protein expression, form a double synergistic mechanism of rapid activation and long-term regulation, and improve the overall sleep-promoting activity. The probiotic protein powder prepared by the application can effectively improve sleep quality, prolong sleep time and shorten sleep latency, and is suitable for the fields of functional food or health products.

The invention discloses a skin-derived staphylococcus coriolis CCSM008 and a prepared metagen with a skin barrier repairing effect, and belongs to the field of microbial technology and medicine. The staphylococcus coriolis CCSM008 provided by the invention shows a remarkable barrier repair effect in the aspect of external use, and the remarkable barrier repair effect is specifically shown as follows: in an in-vitro experiment, the activity of damaged HaCaT cells can be promoted, the gene and protein expression levels of functional proteins and structural proteins after damage are improved, and meanwhile, the expression of sphingomyelinase 1 (SMPD1) and tissue kinin enzyme 7 (KLK7) is remarkably improved. Research finds that the strain regulates expression of skin barrier related proteins and enzymes through TSLP and ERK receptors. The staphylococcus coriolis metagen prepared by the invention has a wide application prospect in the aspects of preparing cosmetics or medicines and the like.