Application of rMBP-NAP in regulation and control of macrophage and direct tumor resistance
A macrophage, anti-tumor technology, applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, peptide/protein components, etc., can solve the problem of no literature disclosure of tumors
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[0020] Experiment 1. rMBP-NAP promotes the proliferation of RAW264.7 macrophages
[0021] Method: CCK-8 method was used to detect the proliferation of RAW264.7 cells
[0022] (1) Collect RAW264.7 cells in good growth state, make a single cell suspension, and adjust the cell concentration to 5×10 4 / mL, 100 μL per well, spread in a 96-well plate, and pre-culture for a period of time to allow the cells to adhere to the wall.
[0023] (2) The cells were treated with different concentrations of rMBP-NAP (10 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL), while PBS was used as a negative control and LPS (1 μg / mL) was used as a positive control. Set up 5 duplicate wells in one group, place at 37°C, 5% CO 2 cultured in an incubator.
[0024] (3) After culturing for 24 hours, add CCK-8 solution, 10 μL / well, place in an incubator and incubate for 4 hours, and measure the absorbance at 450 nm.
[0025] (4) Calculation formula of cell proliferation rate: cell proliferation rate=(experimental ...
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