Application of rMBP-NAP in regulation and control of macrophage and direct tumor resistance

A macrophage, anti-tumor technology, applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, peptide/protein components, etc., can solve the problem of no literature disclosure of tumors

Inactive Publication Date: 2018-04-06
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant research on the effect of rMBP-NAP on macrophages, and there is no literature disclosing its direct anti-tumor use

Method used

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  • Application of rMBP-NAP in regulation and control of macrophage and direct tumor resistance
  • Application of rMBP-NAP in regulation and control of macrophage and direct tumor resistance
  • Application of rMBP-NAP in regulation and control of macrophage and direct tumor resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] Experiment 1. rMBP-NAP promotes the proliferation of RAW264.7 macrophages

[0021] Method: CCK-8 method was used to detect the proliferation of RAW264.7 cells

[0022] (1) Collect RAW264.7 cells in good growth state, make a single cell suspension, and adjust the cell concentration to 5×10 4 / mL, 100 μL per well, spread in a 96-well plate, and pre-culture for a period of time to allow the cells to adhere to the wall.

[0023] (2) The cells were treated with different concentrations of rMBP-NAP (10 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL), while PBS was used as a negative control and LPS (1 μg / mL) was used as a positive control. Set up 5 duplicate wells in one group, place at 37°C, 5% CO 2 cultured in an incubator.

[0024] (3) After culturing for 24 hours, add CCK-8 solution, 10 μL / well, place in an incubator and incubate for 4 hours, and measure the absorbance at 450 nm.

[0025] (4) Calculation formula of cell proliferation rate: cell proliferation rate=(experimental ...

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Abstract

The invention provides application of rMBP-NAP in regulation and control of a macrophage. The inventor accidently finds that the MBP-NAP can promote proliferation of the macrophage and / or enhance pinocytosis capability of the macrophage and / or promote the macrophage to be polarized toward an M1 type. The invention also provides application of MBP-NAP fusion protein in direct tumor resistance.

Description

technical field [0001] The invention belongs to the field of biotechnology. Background technique [0002] Macrophages are widely distributed in the tissues and organs of the body, and play an important role in pathogen defense, inflammatory response, homeostasis maintenance, and injury repair. They can also serve as professional antigen-presenting cells to process and present antigens to activate adaptive immune responses. Depending on the way of activation, macrophages can be polarized into two phenotypes, M1 and M2. Classically activated macrophages (M1 type) activated by LPS or IFN-γ can highly express iNOS, secrete NO, ROI and other killer molecules, and TNF-α, IL-6, IL-12 and other pro-inflammatory factors. The alternative activated macrophages (M2 type) activated by IL-4, etc., secrete a large amount of anti-inflammatory factors such as IL-10 and TGF-β, participate in the resolution of inflammation, tissue remodeling, angiogenesis, and can promote tumor growth. growt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61K47/42A61P13/08A61P35/00
CPCA61K38/164A61K47/42
Inventor 康巧珍刘鑫江慧杜娟王婷鲁吉珂汲振余石雨
Owner ZHENGZHOU UNIV
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