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Fusarium oxysporum extract and application thereof

A Fusarium oxysporum extract technology, applied in the field of biopharmaceuticals, can solve the problem of no immune enhancement effect of Fusarium oxysporum water extract polysaccharides

Active Publication Date: 2012-10-31
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, people's research on Fusarium oxysporum mainly focuses on its pathogenic mechanism, molecular biology and biological control of plants. The latest research shows that polysaccharides from Fusarium oxysporum have antioxidant activity in vitro [Li, P., Luo, C ., Sun, W., et al.In vitro antioxidant activities of polysaccharides from endophytic fungus Fusarium oxysporum Dzf17.African Journal of Microbiology Research, 2011,5:5990-5993], but there is no water extraction of Fusarium oxysporum at home and abroad so far. Reports on Immunoenhancing Effects of Compounds and Their Polysaccharides

Method used

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  • Fusarium oxysporum extract and application thereof
  • Fusarium oxysporum extract and application thereof
  • Fusarium oxysporum extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Mouse mononuclear macrophage RAW264.7 was cultured in DMEM medium containing 10% fetal bovine serum, 1% streptomycin / penicillin at 37°C, 5% CO 2 Growth was incubated in a constant temperature incubator. Adjust RAW264.7 macrophage cell suspension 5×10 3 One per well was inoculated in a 96-well culture plate overnight, and 200 μL of a certain concentration of Fusarium oxysporum water extract or its crude polysaccharide was added, and the culture was continued for 24 hours. The cell viability was detected by MTT method, and the macrophage proliferation ability was calculated by the absorbance ratio of the drug-dosed group and the blank control group.

[0033] The results showed that the aqueous extract of Fusarium oxysporum and the polysaccharide had a significant promotion effect on the proliferation of macrophages in the concentration range of 0.5-128 μg / mL, as shown in Table 1.

[0034] Table 1. Effect of water extract of Fusarium oxysporum and its polysaccharides on ...

Embodiment 2

[0038] RAW 264.7 cells 7.5 x 10 5 Each well was inoculated in a 24-well cell culture plate, and 250 μL of different concentrations of Fusarium oxysporum water extract or its crude polysaccharide was added to each well. The same volume of lipopolysaccharide (LPS, 400ng / mL) and culture solution were used as the positive control group and the blank control group respectively. 2 After incubating in the incubator for 1 h, add 5 μL Rainbow fluorescent particles (about 10×10 per well 6 cells / well) and incubated in the dark for 2 h, the culture medium was discarded, washed twice with PBS, the cells were collected, and the phagocytosis percentage (%) was detected by flow cytometry, and the result was expressed as the ratio of the phagocytosis rate of the solvent control group.

[0039] The results showed that the water extract of Fusarium oxysporum and its polysaccharide could significantly promote the phagocytosis of macrophages at a certain concentration. Water extract (300μg / mL) i...

Embodiment 3

[0041] RAW 264.7 cells 6 x 10 4 Inoculate / well in 96-well cell culture plate, at 37°C, 5% CO 2 After incubating in the incubator for 24 hours, each well was added with different concentrations of Fusarium oxysporum water extract or its crude polysaccharide, and the same volume of LPS (400ng / mL) or culture solution was used as a positive control and a blank control, respectively, and the Griess method was used to determine the macrobiotic concentration. For the amount of NO released by phagocytes, ELISA kits were used to measure the levels of TNF-α and IL-1α in the cell culture supernatant, and the NO release capacity (%) was calculated by the ratio of the absorbance of the LPS positive control group.

[0042] The results showed that the water extract of Fusarium oxysporum (8-128 μg / mL) and its polysaccharide (0.5-128 μg / mL) could significantly (p<0.05) or extremely significantly (p<0.01) promote macrophages to release NO (see Table 2); the water extract and its polysaccharide...

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Abstract

The invention discloses a fusarium oxysporum water extract and a function and an application of a polysaccharide thereof on the aspect of immunological enhancement, which belong to the field of bio-pharmaceuticals. The fusarium oxysporum water extract and the polysaccharide thereof have remarkable immunological enhancement effects, and can be used for promoting macrophagocyte proliferation, improving the macrophagocyte cytophagy function and activating release of immune active factors NO, IL-1alpha and TNF-alpha by macrophagocytes. The fusarium oxysporum water extract and the polysaccharide can be used for assisting in treating immunocompromise diseases and tumors under an immunological enhancement action.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to the effect and application of Fusarium oxysporum water extract and its polysaccharide in enhancing immunity. Background technique [0002] The immune system is the most important defense system for the body to eliminate the invasion of pathogenic microorganisms and eliminate aging, damaged and diseased cells to maintain its own stability. Immunoenhancing drugs can improve the biological activity of immune system tissue cells, increase the growth of immune cells and the synthesis of antibodies, regulate the body's immune function, maintain the balance of the body's internal environment, and thus improve the body's disease resistance. At present, immunoenhancing drugs have been widely used in the adjuvant therapy of anti-tumor, various infectious diseases and secondary immunodeficiency diseases [Hadden, J.W. Immunostimulants. Trends in Pharmacological Sciences, 1993, 14: 169-174]. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P35/00A61P37/04
CPCA61K35/74A61K36/062A61P35/00A61P37/04
Inventor 李绍平冯昆孟兰贞赵静
Owner UNIVERSITY OF MACAU
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