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Preparation method for Sinkiang turnip polysaccharide having immunoregulatory activity

An immunomodulatory activity, polysaccharide technology, applied in allergic diseases, drug combinations, etc.

Inactive Publication Date: 2015-04-01
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pharmacological studies have found that plant polysaccharides have the effects of anti-aging, anti-mutagenesis, immune regulation, anti-radiation and inhibiting the growth of cancer cells. However, so far, systematic research on Xinjiang turnip polysaccharides has rarely been reported at home and abroad.

Method used

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  • Preparation method for Sinkiang turnip polysaccharide having immunoregulatory activity
  • Preparation method for Sinkiang turnip polysaccharide having immunoregulatory activity
  • Preparation method for Sinkiang turnip polysaccharide having immunoregulatory activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Take Xinjiang turnip tubers, wash them, dry them at 30°C, pulverize them, and pass through a 60-mesh sieve to obtain pulverized objects with uniform particles;

[0021] (2) Soak the pulverized product obtained in step (1) with 80% ethanol to remove impurities twice, each time for 4 hours, discard the supernatant by suction filtration, and dry the obtained residue naturally;

[0022] (3) Add distilled water to extract the residue obtained in step (2) at a liquid-to-solid ratio of 40-80mL / g. The extraction temperature is 80-100°C, the time is 1-5h, and the number of extractions is 1-5 times. liquid;

[0023] (4) Suction filtration of the extract obtained in step (3) to get the supernatant;

[0024] (5) Concentrate the supernatant obtained in step (4) to an appropriate volume under reduced pressure at 50°C, add 3 times absolute ethanol to the concentrated solution, precipitate at 4°C overnight, and centrifuge (4000rpm, 10min) to take the precipitate ;

[0025] (6) W...

Embodiment 2

[0032] Optimization of extraction conditions for the preparation of Xinjiang turnip polysaccharides with immunomodulatory activity

[0033] (1) A single factor experiment on the optimization of extraction conditions for the preparation of Xinjiang turnip polysaccharides with immunomodulatory activity

[0034] ①Effect of liquid-material ratio on the extraction rate of Xinjiang turnip polysaccharides

[0035] The liquid-solid ratio was set to five gradients of 40, 50, 60, 70 and 80mL / g, and the extraction temperature, extraction time and extraction times were 90°C, 2h and 1 time, respectively. The polysaccharide extraction rates of different liquid-to-solid ratios are shown in Table 1:

[0036]

[0037] It can be seen from Table 1 that when the liquid-solid ratio exceeds 70mL / g, the extraction rate decreases slowly, so 70mL / g is selected as the center point of the response surface.

[0038] ②Effect of extraction temperature on the extraction rate of Xinjiang turnip polysacc...

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Abstract

The invention relates to a preparation method for Sinkiang turnip polysaccharide having immunoregulatory activity. The method includes the steps that Sinkiang turnip root tubers are dried and smashed, ethyl alcohol with the concentration of 80% is used for removing impurities from the Sinkiang turnip root tubers, the Sinkiang turnip root tubers are subjected to hot water extraction, suction filtration is conducted to extract supernate, absolute ethyl alcohol precipitation and centrifugation are conducted, precipitate is washed sequentially through diethyl ether, ethyl alcohol and acetone and is dried until the constant weight state is reached, and the Sinkiang turnip polysaccharide is obtained. An MTT method is used for analyzing the immunoregulatory activity of the Sinkiang turnip polysaccharide and it is discovered that the Sinkiang turnip polysaccharide has a remarkable effect of promoting mouse peritoneal macrophage proliferation. The preparation method for the Sinkiang turnip polysaccharide has the advantages that the extraction yield is high, cost is low, the structure of the polysaccharide is complete, and no pollution is caused. The prepared Sinkiang turnip polysaccharide has the prominent immunoregulatory activity, thereby having broad application prospects in the food industry and the pharmaceutical industry.

Description

technical field [0001] The invention relates to the technical field of extracting related components from brassica brassica roots, in particular to a method for preparing Xinjiang turnip polysaccharides with immunoregulatory activity. Background technique [0002] Xinjiang turnip belongs to Brassica brassica species of Brassicaceae. It is a biennial herbaceous plant with fleshy roots, spherical, oblate or oblong. The upper end of the appearance is greener, and the lower end is green and white. It has a mustard smell and a spicy taste. The flowering period is from April to May every year, and it is harvested in autumn. It is mainly produced in the southwest of Tianshan Mountains and Northwest Tarim in Xinjiang, China. It is widely planted in southern Xinjiang. It is a traditional medicine and food that is loved by ethnic minorities such as Xinjiang Uyghurs and has a long history of consumption. homologous plants. It is clinically used for nourishing yin and nourishing the lu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/04
Inventor 曾晓雄王伟
Owner NANJING AGRICULTURAL UNIVERSITY
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