Preparation method for Sinkiang turnip polysaccharide having immunoregulatory activity
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
An immunomodulatory activity, polysaccharide technology, applied in allergic diseases, drug combinations, etc.
Inactive Publication Date: 2015-04-01
NANJING AGRICULTURAL UNIVERSITY
View PDF5 Cites 4 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Pharmacological studies have found that plant polysaccharides have the effects of anti-aging, anti-mutagenesis, immune regulation, anti-radiation and inhibiting the growth of cancer cells. However, so far, systematic research on Xinjiang turnip polysaccharides has rarely been reported at home and abroad.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0020] (1) Take Xinjiang turnip tubers, wash them, dry them at 30°C, pulverize them, and pass through a 60-mesh sieve to obtain pulverized objects with uniform particles;
[0021] (2) Soak the pulverized product obtained in step (1) with 80% ethanol to remove impurities twice, each time for 4 hours, discard the supernatant by suction filtration, and dry the obtained residue naturally;
[0022] (3) Add distilled water to extract the residue obtained in step (2) at a liquid-to-solid ratio of 40-80mL / g. The extraction temperature is 80-100°C, the time is 1-5h, and the number of extractions is 1-5 times. liquid;
[0023] (4) Suction filtration of the extract obtained in step (3) to get the supernatant;
[0024] (5) Concentrate the supernatant obtained in step (4) to an appropriate volume under reduced pressure at 50°C, add 3 times absolute ethanol to the concentrated solution, precipitate at 4°C overnight, and centrifuge (4000rpm, 10min) to take the precipitate ;
[0025] (6) W...
Embodiment 2
[0032] Optimization of extraction conditions for the preparation of Xinjiang turnip polysaccharides with immunomodulatory activity
[0033] (1) A single factor experiment on the optimization of extraction conditions for the preparation of Xinjiang turnip polysaccharides with immunomodulatory activity
[0034] ①Effect of liquid-material ratio on the extraction rate of Xinjiang turnip polysaccharides
[0035] The liquid-solid ratio was set to five gradients of 40, 50, 60, 70 and 80mL / g, and the extraction temperature, extraction time and extraction times were 90°C, 2h and 1 time, respectively. The polysaccharide extraction rates of different liquid-to-solid ratios are shown in Table 1:
[0036]
[0037] It can be seen from Table 1 that when the liquid-solid ratio exceeds 70mL / g, the extraction rate decreases slowly, so 70mL / g is selected as the center point of the response surface.
[0038] ②Effect of extraction temperature on the extraction rate of Xinjiang turnip polysacc...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a preparation method for Sinkiang turnip polysaccharide having immunoregulatory activity. The method includes the steps that Sinkiang turnip root tubers are dried and smashed, ethyl alcohol with the concentration of 80% is used for removing impurities from the Sinkiang turnip root tubers, the Sinkiang turnip root tubers are subjected to hot water extraction, suction filtration is conducted to extract supernate, absolute ethyl alcohol precipitation and centrifugation are conducted, precipitate is washed sequentially through diethyl ether, ethyl alcohol and acetone and is dried until the constant weight state is reached, and the Sinkiang turnip polysaccharide is obtained. An MTT method is used for analyzing the immunoregulatory activity of the Sinkiang turnip polysaccharide and it is discovered that the Sinkiang turnip polysaccharide has a remarkable effect of promoting mouse peritoneal macrophage proliferation. The preparation method for the Sinkiang turnip polysaccharide has the advantages that the extraction yield is high, cost is low, the structure of the polysaccharide is complete, and no pollution is caused. The prepared Sinkiang turnip polysaccharide has the prominent immunoregulatory activity, thereby having broad application prospects in the food industry and the pharmaceutical industry.
Description
technical field [0001] The invention relates to the technical field of extracting related components from brassica brassica roots, in particular to a method for preparing Xinjiang turnip polysaccharides with immunoregulatory activity. Background technique [0002] Xinjiang turnip belongs to Brassica brassica species of Brassicaceae. It is a biennial herbaceous plant with fleshy roots, spherical, oblate or oblong. The upper end of the appearance is greener, and the lower end is green and white. It has a mustard smell and a spicy taste. The flowering period is from April to May every year, and it is harvested in autumn. It is mainly produced in the southwest of Tianshan Mountains and Northwest Tarim in Xinjiang, China. It is widely planted in southern Xinjiang. It is a traditional medicine and food that is loved by ethnic minorities such as Xinjiang Uyghurs and has a long history of consumption. homologous plants. It is clinically used for nourishing yin and nourishing the lu...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more