Ginseng derived nano-particles, as well as preparation and application thereof

A nanoparticle and ginseng technology, applied in the field of immune enhancers, can solve the problems of inability to activate immune molecules and inability to fully promote cell-mediated immune responses, and achieve good application prospects, easy operation, and less time-consuming effects

Pending Publication Date: 2017-05-31
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional granulocyte-monocyte-macrophage colony-stimulating factor can only promote the growth of monocyte-macrophages, but it cannot activate TLR and other related i

Method used

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  • Ginseng derived nano-particles, as well as preparation and application thereof
  • Ginseng derived nano-particles, as well as preparation and application thereof
  • Ginseng derived nano-particles, as well as preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1: Preparation of ginseng-derived nanoparticles

[0038] (1) Wash fresh ginseng with clean water, and extract the original pulp through low-speed screw extrusion technology in an environment of 20°C;

[0039] (2) filtering the squeezed raw pulp through a 500-mesh sieve in an environment of 20°C to remove impurities, and collecting the filtrate;

[0040] (3) Centrifuge the collected filtrate at 200×g for 10 minutes, discard the precipitate, and collect the supernatant; then centrifuge the supernatant at 2000×g for 30 minutes, discard the precipitate, and collect the supernatant; Centrifuge at 10,000×g for 60 minutes, discard the precipitate, and collect the supernatant; finally, centrifuge the supernatant at 120,000×g for 60 minutes, and collect the precipitate;

[0041] (4) After the precipitate was resuspended with pH 7.2 phosphate buffer, centrifuge at 120,000×g for 60 minutes to collect the precipitate; , centrifuged for 60 minutes, and the supernatant was ...

Embodiment 2

[0042] Example 2: Preparation of nanoparticles derived from American ginseng

[0043] (1) Wash the fresh American ginseng with clean water, and extract the original pulp by low-speed screw extrusion technology in an environment of 10°C;

[0044] (2) filter the extracted raw pulp through a 200-mesh sieve in an environment of 10°C to remove impurities, and collect the filtrate;

[0045] (3) Centrifuge the collected filtrate at 500×g for 5 minutes, discard the precipitate, and collect the supernatant; then centrifuge the supernatant at 5000×g for 10 minutes, discard the precipitate, and collect the supernatant; Centrifuge at 12,000×g for 30 minutes, discard the precipitate, and collect the supernatant; finally, centrifuge the supernatant at 100,000×g for 90 minutes, and collect the precipitate;

[0046] (4) After the precipitate was resuspended with pH 7.4 phosphate buffer, centrifuge at 100,000×g for 90 minutes to collect the precipitate; , centrifuged for 30 minutes, and the su...

Embodiment 3

[0047] Example 3: Preparation of nanoparticles derived from Panax notoginseng

[0048] (1) Wash the fresh Panax notoginseng with clean water, and extract the original pulp by low-speed screw extrusion technology in an environment of 25°C;

[0049] (2) Filtrating the squeezed raw pulp through a 1000-mesh sieve in an environment of 25°C to remove impurities, and collecting the filtrate;

[0050] (3) Centrifuge the collected filtrate at 500×g for 10 minutes, discard the precipitate, and collect the supernatant; then centrifuge the supernatant at 5000×g for 30 minutes, discard the precipitate, and collect the supernatant; Centrifuge at 12,000×g for 45 minutes, discard the precipitate, and collect the supernatant; finally, centrifuge the supernatant at 200,000×g for 60 minutes, and collect the precipitate;

[0051] (4) After the precipitate was resuspended with pH 7.3 phosphate buffer, centrifuge at 200,000×g for 60 minutes to collect the precipitate; , centrifuged for 45 minutes...

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Abstract

The invention discloses ginseng derived nano-particles, as well as a preparation method and application thereof. The ginseng derived nano-particles have a membrane structure, wherein the particle size range is 150 to 500nm, and the peak particle size is 280 to 350nm. The ginseng derived nano-particle is prepared by performing centrifugation and filtering impurity removal for many times. By the ginseng derived nano-particle, a bone marrow derived monocyte-macrophage can be effectively induced to be proliferated and activated, meanwhile, multiple surfactant molecules such as a TLR2/4 (toll-like receptor 2/4) and a CD80 (cluster of differentiation 80) are up-regulated, and cell factors such as a TNF-a (tumor necrosis factor-a) and an IL-6 (interleukin-6) are secreted. The ginseng derived nano-particle has broad application prospect in development of a medicament for preparing a natural immunoenhancer.

Description

technical field [0001] The invention belongs to the field of immune enhancers, and in particular relates to a ginseng-derived nanoparticle and its preparation and application. Background technique [0002] Microvesicles are a type of extracellular vesicles. Different from exosomes (Exosomes), microvesicles are mainly nano-scale particles with a diameter of about 100-1000 nm produced through the outward budding of the plasma membrane. These nanoparticles often carry a variety of active substances such as protein, DNA, RNA, etc., and play a variety of biological activities. [0003] Immunopotentiators generally refer to substances that can enhance the body's immune response when used alone or together with antigens, and are also called immunomodulators or immunostimulators. Immunopotentiators can work through different pathways, such as enhancing the activity of monocyte-macrophages, enhancing the immunogenicity and stability of antigenic substances, and promoting the synthe...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61K36/344A61K36/36A61K36/537A61K36/808A61K9/14A61P37/04A61P31/18A61P31/06A61P31/10A61P35/00A61P25/00A61P33/02
CPCA61K9/14A61K36/258A61K36/344A61K36/36A61K36/537A61K36/808
Inventor 曹鹏曹萌颜怀江
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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