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Polymer/silver active bonding compound for inhibiting proliferation of activated macrophage, and preparation and applications of bonding compound

A technology of macrophages and polymers, applied in the direction of inorganic active ingredients, drug combinations, drug delivery, etc., can solve the problem of rheumatoid arthritis that does not publicly inhibit the proliferation of activated macrophages, affect the treatment effect of rheumatoid arthritis, Drug toxicity and side effects and other problems can be achieved to improve drug efficacy, relieve joint swelling and inflammation, and reduce side effects

Inactive Publication Date: 2019-12-27
上海百易基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the side effects of drugs affect the therapeutic effect of rheumatoid arthritis
[0005] Nanosilver is a kind of metal nanoparticles, but it is mainly used for antibacterial effects, such as wound healing and gynecological inflammatory diseases. There are no published reports on inhibiting the proliferation of activated macrophages, especially rheumatoid arthritis.

Method used

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  • Polymer/silver active bonding compound for inhibiting proliferation of activated macrophage, and preparation and applications of bonding compound
  • Polymer/silver active bonding compound for inhibiting proliferation of activated macrophage, and preparation and applications of bonding compound
  • Polymer/silver active bonding compound for inhibiting proliferation of activated macrophage, and preparation and applications of bonding compound

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Experimental program
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Effect test

Embodiment 1

[0067] The preparation of embodiment 1 nanometer silver preparation

[0068] Dissolve folic acid in dimethyl sulfoxide (DMSO), add DCC / NHS catalyst, the molar weight of DCC or NHS is 1.1 times that of folic acid, react at room temperature for 4 hours, and activate the carboxyl group; then add amino-modified lipoic acid-polyethylene glycol (LA-PEG 3400 -NH 2 ), reacted at room temperature for 48 hours, suction filtered, dialyzed, and freeze-dried to obtain PEG modified with folic acid (labeled as PEG-FA). Nano elemental silver and PEG-FA were mixed, and stirred at room temperature in the dark for 24 hours to obtain a polymer / silver active bond, that is, a nano silver preparation.

[0069] The particle size of the nanometer elemental silver (marked as AgNPs) of the present invention is 19-22nm, and the particle size of the nanometer silver preparation (marked as FA-AgNPs) is 31-35nm. Particle size measurement method: take the sample solution and place it in a Marlven Nano ZS ...

Embodiment 2

[0072] The stability of embodiment 2 nano silver preparations

[0073] AgNPs were mixed with different concentrations (10-200mM) of sodium chloride (NaCl) solutions. As the concentration of NaCl increased, the color of the AgNPs suspension gradually became light to colorless, and the absorbance value of the plasmon resonance absorption peak also gradually decreased. ( figure 1 D). When FA-AgNPs were mixed with different concentrations (10-200mM) of NaCl solutions, the appearance color did not change, and the absorbance value of the plasmon resonance absorption peak did not change significantly ( figure 1 E). AgNPs and FA-AgNPs were incubated with complete medium containing 10% fetal bovine serum (FBS) for 48 h at 37°C, and there was no significant change in particle size ( figure 1 F). The results showed that FA-AgNPs had good stability in NaCl solution and plasma.

[0074] Part III: Effect Measurement:

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Abstract

The invention discloses a polymer / silver active bonding compound for inhibiting the proliferation of activated macrophage, and a preparation process and applications of the bonding compound. The polymer / silver active bonding compound consists of nano elemental silver and a hydrophilic polymer modified by a target head. The polymer / silver active bonding compound is excellent in stability and can inhibit the proliferation of activated macrophage, so that the polymer / silver active bonding compound is good in anti-rheumatoid arthritis effect in vitro and in vivo.

Description

technical field [0001] The invention relates to the field of nano-biotechnology, in particular to the application of nano-silver in treating rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis is an autoimmune disease, mainly involving the small joints of the whole body, such as wrist joints, finger joints, ankle joints, etc., causing joint swelling, pain, and dysfunction; its pathological features are synovial tissue hyperplasia, inflammatory cells and inflammatory cytokine infiltration. The pathogenesis of rheumatoid arthritis is still unclear, and it may be related to genetic, environmental and other factors. [0003] In the development of rheumatoid arthritis, inflammatory cells infiltrate, macrophages and vascular endothelial cells are activated. Some receptors are highly expressed on the surface of activated macrophages, such as folate receptor (FR), selectin E, and CD44 molecules. Inflammatory cells produce a large number of proinflammatory cyt...

Claims

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Application Information

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IPC IPC(8): A61K33/38A61K47/54A61K47/69A61P19/02A61P37/02A61P29/00
CPCA61K33/38A61K47/545A61K47/549A61K47/6933A61K47/6935A61P19/02A61P29/00A61P37/02
Inventor 周文虎徐有伟杨宜华丁劲松
Owner 上海百易基因科技有限公司
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