A polysaccharide sm-0.2m and anti-tumor product prepared therefrom

A technology of polysaccharides and products, which is applied to the polysaccharide SM-0.2M in raspberry polysaccharides and its preparation field, can solve the problems that have not been studied in depth, and achieve the effects of enhancing phagocytosis, promoting macrophage proliferation, and improving immunity

A technology of polysaccharides and products, which is applied to the polysaccharide SM-0.2M in raspberry polysaccharides and its preparation field, can solve the problems that have not been studied in depth, and achieve the effects of enhancing phagocytosis, promoting macrophage proliferation, and improving immunity

CN112358553BActive Publication Date: 2021-10-01QINGHAI UNIVERSITY
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  • A polysaccharide sm-0.2m and anti-tumor product prepared therefrom
  • A polysaccharide sm-0.2m and anti-tumor product prepared therefrom
  • A polysaccharide sm-0.2m and anti-tumor product prepared therefrom

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Experimental program
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Effect test

Embodiment 1

[0052] 1. Extraction of Raspberry Crude Polysaccharide

[0053]Dried raspberry frozen fruit, crushed and removed seeds, added 1-10 times of petroleum ether (boiling range 60-90°C) to degrease, added 85% ethanol at 50-70°C to reflux to remove monosaccharides and pigments, and Add 5-30 times of pure water to the filtered residue for ultrasonic extraction (power 50-70W, temperature: 50-90°C, processing time: 60min-100min), centrifuge to get the supernatant, extract the filter residue twice according to the original conditions, and combine the filtrate , concentrated under reduced pressure to 1 / 4 of the original volume, added 3 to 5 times the volume of 95% ethanol, stood at 4 degrees Celsius for 24 hours, filtered to collect the filter residue, and freeze-dried to obtain the raspberry crude polysaccharide.

[0054] 2. Separation and purification of raspberry homogeneous polysaccharide fraction

[0055] The DEAE Sepharose Fast Flow filler is used for polar separation of polysaccha...

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Abstract

The invention discloses a polysaccharide SM‑0.2M, which is separated from raspberry polysaccharides, and its specific sugar chain structure is identified. It is verified by experiments that the polysaccharide SM‑0.2M can promote the proliferation of macrophages, and can promote macrophages The cells release NO, up-regulate the expression of TNF-α and IL-6, and then enhance the phagocytosis of macrophages, improve immunity, and help macrophages play an anti-tumor effect, which can be used to prepare related products, and to further prove The structure-activity relationship of raspberry polysaccharides and the development of polysaccharide antitumor drugs and immune adjuvants provide reference.

Description

technical field [0001] The invention belongs to the technical field of natural products, and in particular relates to polysaccharide SM-0.2M in raspberry polysaccharide and its preparation method and application. Background technique [0002] Plant polysaccharides, also known as plant polysaccharides, are polysaccharides with a degree of polymerization of more than 10 produced by plant cell metabolism. Important biological macromolecules in living organisms are one of the basic substances to maintain the normal operation of life activities. [0003] At the same time, plant polysaccharides are recognized as natural macromolecules with various biological activities, and most plant polysaccharides have significant immunomodulatory and antitumor activities in vivo. They can not only directly affect the functions of various immune cells such as macrophages, T lymphocytes, B lymphocytes, and natural killer cells, but also regulate the production of related cytokines and complemen...

Claims

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Application Information

Patent Timeline
01 Oct 2021
Publication
CN112358553B
IPC
C08B37/00; A61K31/715; A61P37/04; A61P35/00
CPC
A61K31/715; A61P35/00; A61P37/04; C08B37/0003; C08B37/006
Inventors
杨永晶; 王学红