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Sea intestine gum as well as preparation method and application thereof

A sea intestine and buffer technology, applied in the field of sea intestine glue and its preparation, can solve the problems of unfavorable large-scale popularization or use, inability to use drugs or experimental reagents, single components and functions, etc., to promote macrophage proliferation, The effect of widening the utilization route and simple preparation method

Active Publication Date: 2021-07-16
威海银河生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The inventor studied the existing animal extracts or gelatin products and found that the existing animal extracts have single components and functions, and only have certain nutritional value, so they cannot be used for medicine or experimental reagents
Some animal gum products are complicated to prepare and need to be heated and pressurized to prepare, and the yield of gum is low, which is not conducive to large-scale promotion or use

Method used

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  • Sea intestine gum as well as preparation method and application thereof
  • Sea intestine gum as well as preparation method and application thereof
  • Sea intestine gum as well as preparation method and application thereof

Examples

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preparation example Construction

[0024] In a first aspect, the present invention provides a method for preparing sea intestine gum, comprising the following steps:

[0025] The enzymolysis solution is obtained by mixing sea intestine, buffer solution and protease, heating at high temperature and drying.

[0026] The purpose of enzymatic hydrolysis of sea intestine is to increase the yield of sea intestine glue, the purpose of high temperature heating is to remove fishy smell, and the purpose of drying is to form glue, heat and dry sea intestine glue in an oven, and cut the dried glue into long strips Continue to dry, turn the rubber block regularly during the period, keep the same wind and low humidity, prevent the rubber block from getting wet, until it is completely dry and solid, that is, the finished sea intestine glue is obtained.

[0027] In order to improve the quality and purity of the sea intestine glue from the source, the sea intestine also includes a pretreatment step before mixing with the buffer...

Embodiment 1

[0053] Example 1: Research on enzymatic hydrolysis conditions of sea intestine glue:

[0054] (1) Selection of hydrolase: The study was divided into three groups, using neutral protease, papain and bromelain respectively.

[0055] (2) Sea sausage pretreatment: Take 2 kg of fresh sea sausage, wash it with tap water, remove the viscera, etc., and leave the body wall muscle for later use. Then tap water and distilled water were washed repeatedly until no foam was produced, and the water in the sea intestine was dried and weighed.

[0056] (3) Enzymolysis: The research is divided into using a homogenizer to homogenate, adding phosphate buffer (pH 7.0) of 1 times the mass of sea intestine, and then adding 3% neutral protease, papain, bromelain, respectively, After stirring evenly, add the sea sausage, place in a water bath at a constant temperature of 50°C for 4 hours, and stir continuously during the whole process to obtain the enzymatic hydrolysis solution. Subsequently, the te...

Embodiment 2

[0063] (1) Sea sausage pretreatment: Take 2 kg of fresh sea sausage, wash it with tap water, remove the viscera, etc., and leave the body wall muscle for later use. Then use tap water and distilled water to elutriate repeatedly until no foam is produced, control the moisture in the sea intestine and then weigh it.

[0064] (2) Enzymolysis: Use a homogenizer to homogenate, add phosphate buffer (pH 7.0) that is 1 times the weight of sea intestine, then add 3% bromelain, stir well, add sea intestine, and place in a constant temperature water bath at 40°C 5h, stirring continuously during the whole process, to obtain the enzymatic hydrolysis solution. Subsequently, the temperature of the water bath was adjusted to 80° C. for 4 minutes to inactivate the enzyme and stop the reaction. Then, the enzymolysis solution was rapidly cooled to below 30°C and left for 30 minutes.

[0065] (3) Deodorization: Centrifuge the sea intestine hydrolyzate at room temperature (1000rpm, 20min), keep ...

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Abstract

The invention relates to the field of food processing and application, in particular to sea intestine gum as well as a preparation method and application thereof. Collagen from sea intestines is extracted by adopting an enzyme method, and a concentrated gel product is further prepared. Activity of the product is evaluated by measuring a proliferation activity of the product on macrophages, and it is found that the product can significantly promote proliferation of the macrophages. The sea intestine gum and the preparation method thereof not only prolong shelf life of sea intestines, but also facilitate further development of sea intestine products, increase resource utilization, and provide a basis for future sea intestine research and resource development.

Description

technical field [0001] The invention relates to the field of food processing and application, in particular to a sea intestine gum and its preparation method and application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] With the development of the economy, people's income levels are gradually increasing, and healthy living has gradually become the mainstream of life. At the same time, the fast-paced and high-pressure social life has spawned a large number of patients with chronic diseases, and the growing demand has led to the rapid development of the food health care industry. [0004] Glue is a dosage form with a long history in traditional Chinese medicine. It uses...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/14A61K38/39A61P37/04
CPCC12P21/06C07K14/78A61K38/39A61P37/04
Inventor 宋淑亮吉爱国赵佳锐李霞程泽宇刘家齐
Owner 威海银河生物技术有限公司
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