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Sea cucumber polypeptides and application thereof

A sea cucumber polypeptide, macrophage technology, applied in the application, peptide, peptide/protein components and other directions, to achieve a significant effect of macrophage proliferation

Active Publication Date: 2020-11-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors found that so far, there are few research reports on the effect of sea cucumber polypeptides on macrophages

Method used

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  • Sea cucumber polypeptides and application thereof
  • Sea cucumber polypeptides and application thereof
  • Sea cucumber polypeptides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0133] Example 1 P1 (polypeptide sequence 5'-3' is VWDGVGSGR, SEQ ID NO.1) research results of sea cucumber synthetic peptide on macrophage proliferation rate

[0134] MTT assay was used to detect the effect of P1 on the proliferation activity of macrophages. Experimental results such as figure 2 As shown, P1 significantly promoted the proliferation of macrophages in a concentration-dependent manner at 25 μg / ml-400 μg / ml, showing a linear upward trend. The value-added rate of P1 at 25μg / ml, 100μg / ml, and 400μg / ml was 3.44%, 11.57%, and 17.78%, respectively. After t-test test, when P1 was at 100μg / ml, P<0.05, there was a significant difference; When P1 is at 400μg / ml, P<0.01, there is a very significant difference.

Embodiment 2

[0135] Example 2 Research results of P2 (polypeptide sequence 5'-3' is WDGVVGSGR, SEQ ID NO.2) sea cucumber synthetic peptide on macrophage proliferation rate

[0136] MTT assay was used to detect the effect of P2 on the proliferation activity of macrophages. Experimental results such as image 3 As shown, P2 can significantly promote the proliferation of macrophages at 25μg / ml-400ug / ml. The value-added rate of P2 at 25μg / ml, 100μg / ml, and 400μg / ml was 9.15%, 18.87%, and 17.92%, respectively. After t-test test, when the concentration of P2 is 25μg / ml, P<0.05, there is a significant difference; when the concentration of P2 is 100μg / ml, P<0.01, there is a very significant difference; when the concentration of P2 is 400μg / ml, P<0.001, there is a very significant difference.

Embodiment 3

[0137] Example 3 Research results of P3 (polypeptide sequence 5'-3' is WDGVGSVGR, SEQ ID NO.3) sea cucumber synthetic peptide on macrophage proliferation rate

[0138] MTT assay was used to detect the effect of P3 on the proliferation activity of macrophages. Experimental results such as Figure 4 As shown, P3 can significantly promote the proliferation of macrophages at 25 μg / ml-400 μg / ml. The value-added rate of P3 at 25μg / ml, 100μg / ml, and 400μg / ml was 22.50%, 33.76%, and 25.77%, respectively. And when the concentration is 25μg / ml, 40μg / ml, P<0.05, there is a very significant difference; when the concentration is 400μg / ml, P<0.001, there is a very significant difference.

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Abstract

The present invention provides sea cucumber polypeptides and an application thereof, and belongs to the technical field of biological pharmacy. The provided sea cucumber polypeptides can be used for preventing or treating diseases related to macrophages, including but not limited to body local tissue infection or lesion and autoimmune diseases caused by pathogenic microorganisms such as viruses and the like, by promoting proliferation of the macrophages, ; Meanwhile, the sea cucumber polypeptide can also be used as a macrophage proliferation promoter to promote in-vitro culture of macrophages.A plurality of the sea cucumber polypeptides with activity of promoting macrophage proliferation are artificially synthesized, so that the sea cucumber polypeptides have good practical application value.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and specifically relates to sea cucumber polypeptides and applications thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The marine environment occupies 95% of the biosphere and is an important source of new marine natural products, which has broad prospects for the development of a large number of efficient new and cheap compounds. In order to resist the extreme harsh environment in the ocean such as temperature, high salinity, pressure, hypoxia, etc., marine organisms produce a large number of chemical substances with special structures and biological activities, which make...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12N15/12C12N5/0786A61K38/08A61P31/00A61P37/02A23L33/18
CPCC07K7/06C12N5/0645A61P31/00A61P37/02A23L33/18C12N2501/999A61K38/00A23V2002/00A23V2200/324A23V2200/30
Inventor 宋淑亮吉爱国
Owner SHANDONG UNIV
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