A Rapid Preparation Method of Dry Silver Stained Samples of Freshwater Ciliates

A ciliate and silver dyeing technology, which is applied in the biological field, can solve the problems of failure of film production, strict requirements for egg white coating thickness and difficult control during egg white preparation and storage time, and achieves the effect of reducing the cost of raw materials and shortening the production cycle.

Active Publication Date: 2021-06-04
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method improves the quality of tablet production, but it is difficult to control because of strict requirements on egg white preparation and storage time and the thickness of egg white coating, which often leads to tablet production failure.

Method used

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  • A Rapid Preparation Method of Dry Silver Stained Samples of Freshwater Ciliates
  • A Rapid Preparation Method of Dry Silver Stained Samples of Freshwater Ciliates
  • A Rapid Preparation Method of Dry Silver Stained Samples of Freshwater Ciliates

Examples

Experimental program
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Embodiment 1

[0036] In this embodiment, taking trichotillophorus as an example, a rapid preparation method for dry silver-stained samples of freshwater ciliates is introduced, which includes the following steps:

[0037] Step 1: Use a micropipette to pick and collect trichomonas and place them in distilled water drops for 5 minutes; then transfer them to new distilled water drops and wash them twice for 5 minutes each time;

[0038] Step 2, absorb the insect liquid and drop it on the clean glass slide treated with polylysine, absorb the excess liquid as much as possible, and divide the insect liquid into several small pieces with an eyebrow pencil (made by sticking the eyebrows on the silver acupuncture needles with gum). Drops, and adjust the position of the worms as needed, pay attention to avoid the two worms sticking together, and then quickly dry the worms with the ear washing ball;

[0039] Step 3, add 1 drop of silver nitrate solution with a mass concentration of 2wt% to cover the w...

Embodiment 2

[0045] This embodiment takes tube worms as an example, and introduces a rapid preparation method for freshwater ciliate dry silver-stained samples, including the following steps:

[0046] Step 1: Use a micropipette to pick and collect the worms and place them in a drop of distilled water for 5 minutes; then transfer them to a new drop of distilled water and wash them twice for 5 minutes each time;

[0047] Step 2, absorb the insect liquid and drop it on the clean glass slide treated with polylysine, absorb the excess liquid as much as possible, and divide the insect liquid into several small pieces with an eyebrow pencil (made by sticking the eyebrows on the silver acupuncture needles with gum). Drops, and adjust the position of the worms as needed, pay attention to avoid the two worms sticking together, and then quickly dry the worms with the ear washing ball;

[0048] Step 3, adding 1 drop of silver nitrate solution with a mass concentration of 2wt% to cover the worm body fo...

Embodiment 3

[0054] In this example, taking intestinal kidney worms as an example, a rapid preparation method for dry silver-stained samples of freshwater ciliates is introduced, which includes the following steps:

[0055] Step 1: Use a micropipette to pick and collect intestinal kidney worms and place them in distilled water drops for 5 minutes; then transfer them to new distilled water drops and wash them twice, 5 minutes each time;

[0056] Step 2, absorb the insect liquid and drop it on the clean glass slide treated with polylysine, absorb the excess liquid as much as possible, and divide the insect liquid into several small pieces with an eyebrow pencil (made by sticking the eyebrows on the silver acupuncture needles with gum). Drops, and adjust the position of the worms as needed, pay attention to avoid the two worms sticking together, and then quickly dry the worms with the ear washing ball;

[0057] Step 3, adding 1 drop of silver nitrate solution with a mass concentration of 1 wt...

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Abstract

The invention discloses a rapid preparation method for dry silver-stained samples of freshwater ciliates, belonging to the field of biotechnology. The method of the present invention comprises: (1) picking and collecting ciliates, and cleaning the surface of the ciliates; (2) adhering the clean worm liquid obtained from cleaning to the surface of a clean glass slide treated with polylysine, and removing the ciliate liquid Divide into several small drops and blow dry; (3) Add silver nitrate solution dropwise to the surface of the worm body, and clean the residue after standing; (4) Use a halogen cold light source to irradiate and develop; (5) Rinse the scum and dry it; (6) Add neutral gum dropwise to the surface of the worm body, add a cover slip and seal the slide. The method of the invention can efficiently and high-quality prepare dry silver-stained samples of freshwater ciliates, and at the same time greatly simplifies the film-making steps, and the invention does not need to use a developing solution, thereby saving raw material costs. In addition, the sample of the present invention is irradiated and developed under a cold halogen light source after being stained with silver nitrate, which is not affected by weather factors and is easier to control.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for preparing a protozoan silver-stained sample, more specifically, the invention relates to a rapid preparation method for a freshwater ciliate dry silver-stained sample. Background technique [0002] Ciliata is a phylum with the largest variety, the most complex structure and the highest level among the single-celled protozoa. They are named ciliates because of the presence of cilia in at least one stage of their life cycle and the use of cilia as locomotion and feeding organs. Although ciliates are very small and can only be observed with a microscope, they play an extremely important role in the biological world and the natural environment, and are also closely related to our human life. Some free-living taxa in ciliates are important components of microfood webs in planktonic and benthic ecosystems, and play an important pivotal role in the process of material cycle and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 李明胡光冉王桂堂邹红李文祥吴山功赵威山汪润秋
Owner INST OF AQUATIC LIFE ACAD SINICA
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