Bionic in-situ regeneration repair nano sticking patch and preparation method and application thereof

Abstract

The invention relates to a bionic in-situ regeneration repair nano sticking patch, which is formed by overlapping an layer A with a cationic group material and a layer B with an anionic group material sequentially, wherein the sticking patch comprises 20 to 80 percent by mass of the A layer with the cationic group material and the balance of the layer B with the anionic group material; layer A with the cationic group material comprises at least one of polysaccharide with a cationic group, protein with the cationic group or opypeptide (polypeptide) with the cationic group; layer B with the anionic group material comprises at least one of polysaccharide with an anionic group, protein with the anionic group or opypeptide (polypeptide) with the anionic group; and the sticking patch comprises not more than 10 percent by mass of added functional factors and / or functional polypeptide. The raw materials used by the invention are materials which can be degraded in vitro and have biocompatibility. The sticking patch is flexible, has good tissue adhesivity, is suitable for concave-convex surfaces of visceral organs, has strong stretching resistance and air pressure resistance, is suitable for application of sticking, sealing, leak stoppage, hemostasia, isolation, repair, adhesion prevention and artificial meninges of defect tissues and can also be used as a slowly-releasing carrier of a medicament and a nano-grade tissue engineering support material.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a nano-patch composed of a material with anion groups, a functional factor and / or a functional polypeptide, and a material with a cationic group. It also provides the preparation method of the nanopatch and its application in the sticking, sealing, plugging, hemostasis, isolation, repair, prevention of adhesion, anti-infection and artificial meninges of defective tissue in surgical operations, and it can also be used as a sustained release of drugs. Carriers and nanoscale tissue engineering scaffold materials. Background technique [0002] In surgical operations, it is often necessary to seal, repair and prevent adhesion of defects and damaged tissues. The existing products include film and gel products of carboxymethylcellulose, chitosan, polylactic acid, collagen and fibrin glue. To a certain extent, the product has played a positive role in the sealing, repairi...

AI Technical Summary

Problems solved by technology

However, there are many defects in its products that affect the performance of its functions. For example, the thickness of collagen sponge or membrane products ranges from 0.5mm to 5mm, resulting in soft products and poor tissue adhesion. Tensile strength makes it inconvenient for clinical surgeons to operate. In order to obtain certain mechanical properties, cross-linking agents such as glutaraldehyde must be added. In addition to certain tissue toxicity, cross-linking agents can easily lead to calcification; animal-derived proteins These products have relatively large safety risks (such as viruses, pathogenic microorganisms, etc.) and adverse reactions such as human allergies caused by protein macromolecules; the mechanical strength of carboxymethyl cellulose and chitosan products is not enough, and the degradation time in vivo is either too short or too long. long; and the degradation of polylactic acid products produces acidic products, etc.; therefore, the development of an innovative product that overcomes the defects of existing membrane products has very important clinical application value