A kind of human vascular endothelial cell culture medium and culture method thereof

Abstract

The invention provides a human vascular endothelial cell culture solution. The human vascular endothelial cell culture solution is prepared from a basic culture medium, fetal calf serum, human recombined epidermal growth factors, human recombined fibroblast growth factors, vascular endothelial growth factors, insulin-like growth factors, vitamin C, cortisol, cholera toxin, aminoethanol and ethanolamine phosphate. Compared with a traditional vascular endothelial cell culture medium, the human vascular endothelial cell culture solution has the advantages that the culture solution can be suitable for multiple kinds of vascular endothelial cells, cell proliferation is effectively promoted, the division time is effectively shortened, the characteristics of the vascular endothelial cells are maintained, the passage number of the in-vitro human vascular endothelial cells is greatly increased, and the survival time of the in-vitro human vascular endothelial cells is greatly prolonged; meanwhile, all the ingredients of the cell culture solution can be obtained in a general laboratory, preparation is convenient, a special finished product culture medium is not needed, therefore, the cost for culturing the endothelial cells is greatly reduced, and the human vascular endothelial cell culture solution is suitable for application and popularization.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a human vascular endothelial cell culture medium and a culture method thereof. Background technique [0002] Vascular endothelial cells not only participate in the mediation of vascular permeability and blood coagulation, but also play an important role in many processes such as immune mediation, transplant rejection, tumor metastasis, and inflammation. In scientific research, in vitro culture is often used to study vascular endothelial cells. Since most people still do not recognize the use of immortalized vascular endothelial cell lines, it is still routine to use purified vascular endothelial cells from humans for research. However, in the culture of vascular endothelial cells, the cell proliferation is often slow, and the properties of the cells change or die after several generations, which increases the research cost and prolongs the research time. [0003] The in vi...

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Problems solved by technology

At present, the relatively mature endothelial cell culture media on the market mainly include EGM and EGM-2 series of Lonza Company, and ECM culture medium of Sciencell. effect, but the formula is secret and expensive

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