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Aged rat brain vascular endothelial cell culture fluid

A technology for cerebrovascular endothelial and cell culture, which is applied in the field of cerebrovascular endothelial cell culture medium in aged rats, and can solve the problems that the sample object and culture technology cannot meet the aged blood-brain barrier model

Inactive Publication Date: 2013-04-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The precedent of successful culture of cerebrovascular endothelial cells in aged rats (19-22 months, 900g-1200g) has not been reported so far; the existing material objects and culture techniques cannot meet the needs of establishing the aged blood-brain barrier model in vitro and studying the effects of the aged blood-brain barrier. Physiological properties, and requirements for blood-brain barrier-related degeneration in geriatric neurological diseases

Method used

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  • Aged rat brain vascular endothelial cell culture fluid
  • Aged rat brain vascular endothelial cell culture fluid
  • Aged rat brain vascular endothelial cell culture fluid

Examples

Experimental program
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Effect test

Embodiment 1

[0047] A kind of old rat cerebral vascular endothelial cell culture fluid, on the basis of every 100 milliliters of DMEM culture medium, add the following components:

[0048] 30ml of plasma-derived serum, 100μl of penicillin-streptomycin solution, 1.0ml of L-glutamine, 100μl of gentamycin, 1.5ml of heparin, 150μl of basic fibroblast factor, 1.0ml of endothelial cell growth supplementation factor, 400μl Vitamin C, 200 μl vascular endothelial growth factor, 200 μl insulin-like growth factor-1, 200 μl endothelial factor;

[0049] Add the above components to the DMEM medium.

Embodiment 2

[0051] A kind of old rat cerebral vascular endothelial cell culture fluid, on the basis of every 100 milliliters of DMEM culture medium, add the following components:

[0052] 20ml of plasma-derived serum, 100μl of penicillin-streptomycin solution, 1.0ml of L-glutamine, 100μl of gentamycin, 1.0ml of heparin, 100μl of basic fibroblast factor, 1.0ml of endothelial cell growth supplementation factor, 200μl Vitamin C, 100 μl vascular endothelial growth factor, 100 μl insulin-like growth factor-1, 100 μl endothelial factor;

[0053] Add the above components to the DMEM medium.

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Abstract

The invention relates to an aged rat brain vascular endothelial cell culture fluid. On the basis of each 100 ml of DMEM (Dulbecco Modified Eagle Medium) culture medium, components including plasma derived serum (PDS), penicillin-streptomycin, L-glutamine, gentamicin, heparin, an alkaline fiber growth factor, an endothelial cell growth replenishing factor, vitamin C, a vascular endothelial growth factor, an insulin-like growth factor-1 and an endothelium growth factor are added. According to the aged rat brain vascular endothelial cell culture fluid, the PDS is adopted for replacing FBS (Fetal Bovine Serum), beef calf serum or horse serum in the traditional culture fluid to ensure that the purity of brain vascular endothelial cells is increased by 30 percent; and the vitamin C and puromycin are added to ensure that the brain vascular endothelial cells grow more rapidly and purely.

Description

technical field [0001] The invention relates to a culture medium of cerebrovascular endothelial cells of aged rats, which belongs to the field of biotechnology. Background technique [0002] The existing primary culture of rat cerebrovascular endothelial cells is obtained from neonatal rats (<10 days, 35-50g), juvenile rats (<3weeks, 80-100g) or young adult rats (2-3 months old, 200g- 300g), and the cultured cerebrovascular endothelial cells can only be passaged once and cannot be frozen. Using rat cerebrovascular endothelial cells as experimental materials leads to the disadvantages of long time and high experimental cost. For example: Bowman PD, Betz AL, Ar D, Wolinsky JS, Penney JB, Shivers RR, Goldstein GW. 1981. Primary Culture of Capillary Endothelium From Rat Brain. INVITRO. 17(4), 353-362. [0003] The precedent of successful culture of cerebrovascular endothelial cells in aged rats (19-22 months, 900g-1200g) has not been reported so far; the existing material...

Claims

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Application Information

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IPC IPC(8): C12N5/071
Inventor 王丽梅崔敏王越陈哲宇
Owner SHANDONG UNIV
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