Human glomerular endothelial cell culture and drug detection toxicity evaluation method based on micro-fluidic chip

Abstract

The invention relates to a human glomerular endothelial cell culture and drug detection toxicity evaluation method based on a micro-fluidic chip, and the specific culture method comprises the following steps: carrying out cell culture and test by using a micro-fluidic cell chip analyzer, and specifically comprises the following steps: 1, preprocessing the chip; step 2, cell inoculation; step 3, cell culture; after cell culture is completed, successfully cultured cells are used for subsequent drug evaluation detection, and the method specifically comprises the following steps: step 1, preparation for detection; 2, detecting the concentration of LDH; step 3, activity detection; in addition, living and dead cell staining, immunofluorescence detection and inflammatory cytokine detection can also be used, the temperature and gas components of a cell growth area can be accurately regulated and controlled by implementing the steps, an external incubator is omitted, long-term continuous observation of cells under a microscope is achieved, and the detection accuracy is improved. The microfluidic cell model can better simulate the human body microenvironment, and the drug evaluation effect is more accurate.

Description

Technical field: [0001] The invention relates to the fields of cell culture and drug toxicity detection and evaluation, in particular to a microfluidic chip-based culture of human glomerular endothelial cells and a method for drug detection and toxicity evaluation. Background technique [0002] The poor predictive ability of the existing in vitro toxicology evaluation models hinders the progress of pharmaceutical research and development to a large extent. Establishing and developing reliable in vitro toxicology evaluation alternative models can effectively improve the accuracy of evaluation data, but the existing It is necessary to determine its drug efficacy and safety or its evaluation data through a series of toxicological evaluation methods including animal experiments and in vitro experiments. The existing technology has not yet established a human glomerular endothelial cell model that mimics the human environment, resulting in preclinical The lack of models and their...

AI Technical Summary

Problems solved by technology

[0002] The poor predictive ability of the existing in vitro toxicology evaluation models hinders the progress of pharmaceutical research and development to a large extent. Establishing and developing reliable in vitro toxicology evaluation alternative models can effectively improve the accuracy of evaluation data, but the existing It is necessary to determine its drug efficacy and safety or its evaluation data through a series of toxicological evaluation methods including animal experiments and in vitro experiments. The existing technology has not yet established a human glomerular endothelial cell model that mimics the human environment, resulting in preclinical The lack of models and their poor predictive ability have led to the failure of a large number of drugs in clinical trials and the increase in the cost of developing new drugs. Reliable in vitro toxicological evaluation of alternative models and cultivation in a human-like environment are urgent needs in the field of pharmaceutical research and development

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