Nano probe transfected adipose-derived cell and application thereof in preparing MRI (magnatic resonance imaging) dynamic tracer

Abstract

The invention belongs to the field of cytobiology, and relates to a nano probe transfected adipose-derived cell and application thereof in preparing an MRI (magnatic resonance imaging) dynamic tracer. The adipose-derived progenitor / stem cell is separated and screened from adipose tissues, the phenotype of the adipose-derived progenitor / stem cell is identified by stream mode, immune fluorescence, molecular biology and other methods, and the inward epidermic cell differentiation is induced. After the nanoparticle labeled adipose-derived endodermis progenitor / stem cell is prepared into an MRI dynamic tracer, the in-vivo transplantation vascular injury restoration test, which is used for evaluating vascular endodermis conditions by transmission electron microscope, immunofluorescence, real-time PCR (polymerase chain reaction) and other methods according to the in-vivo survival, migration, outcome and restoration vascular injury conditions of the nuclear magnetic resonance dynamic tracing adipose-derived endothelium progenitor / stem cell at different time points, proves that the nano probe transfected adipose-derived cell can illuminate the mechanism of the adipose-derived endodermis progenitor / stem cell in vascular injury restoration. The nano probe transfected adipose-derived cell can provide new practice data for vascular injury treatment as well as new seed cell sources and experimental reference.

Description

technical field

[0001] The invention belongs to the field of cell biology, and relates to adipose-derived cells transfected with nanoprobes and its application, in particular to a method for separating and obtaining adipose-derived cells from adipose tissue, transfecting them with nanoprobes and preparing MRI dynamics. Use in tracer. Background technique

[0002] It is known that the heart and cerebrovascular injury caused by atherosclerosis (AS) seriously affects and threatens human health; in an aging society, heart and cerebrovascular diseases have become the number one cause of human death. Therefore, the prevention and treatment of AS and vascular injury has always been the focus and difficulty of research. The traditional view is that lipid deposits in the subintima of arteries and atherosclerotic plaques progress, resulting in high stenosis of vascular lumens, tissue ischemia and hypoxia, and ultimately acute cardiovascular and cerebrovascular events. However, in re...

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