Method for identifying Fujian Guanxi honey pomelos by chromatography method

A technique of chromatography and honey pomelo, applied in the field of analytical chemistry chromatography, to achieve scientific and reliable results, simple operation, and accurate qualitative results

Active Publication Date: 2017-02-22
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the detection and identification of honey pomelo by gas chromatography or liquid chromatography

Method used

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  • Method for identifying Fujian Guanxi honey pomelos by chromatography method
  • Method for identifying Fujian Guanxi honey pomelos by chromatography method
  • Method for identifying Fujian Guanxi honey pomelos by chromatography method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Using gas chromatography and hydrogen flame ion detector to identify the method of Fujian Guanxi honey pomelo, the specific operation steps are as follows:

[0050] 1. The initial temperature of the chromatographic column of the gas chromatograph is room temperature, and the temperature rises at a rate of 10°C / min to a temperature of 50-280°C. In order to meet the complete separation of the peaks of all components in the sample, the optimal analysis of the gas chromatogram is determined in combination with the boiling point of each substance. The conditions are as follows:

[0051] Gas Chromatography Analyzer Analysis Conditions

[0052] Chromatographic column: 30m×0.25mm DB-17 column; carrier gas: N 2 ; Carrier gas flow rate: 1mL / min; Chromatographic column temperature: 120°C; Injection volume: 1μL; Vaporization chamber temperature: 260°C;

[0053] 2. Preparation of external standard solution: according to the mass fraction of external standard, weigh different masse...

Embodiment 2

[0056] The method for identifying Fujian Guanxi honey pomelo by liquid chromatography-ultraviolet detector, the specific operation steps are as follows:

[0057] 1. The liquid chromatograph uses 254nm as the ultraviolet absorption wavelength, gradient elution, in order to meet the complete separation of peaks of all components in the sample, combined with the polarity of each substance, determine the optimal analysis conditions of liquid chromatography as follows:

[0058] Analysis conditions of liquid chromatography analyzer

[0059] Chromatographic column: Zorbax Eclipse XDB C18 250mm×4.6mm×5μm chromatographic column; mobile phase: A is aqueous phase, B is acetonitrile; elution flow rate: 1mL / min; column temperature: 30°C; injection volume: 10μL; gradient Elution program: water A%: acetonitrile B% is 70:30, elution is 0-15min, water A%: acetonitrile B% is 40:60, elution is 15-20min, water A%: acetonitrile B% is 40: 60, elution 20-40min, water A%: acetonitrile B% is 20:80, e...

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PUM

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Abstract

The invention discloses a method for identifying Fujian Guanxi honey pomelos by a chromatography method. According to the method, firstly, acetonitrile is used for extracting chemical ingredients in each position of outer peels, endodermis, endocarp, pulp and the like of the honey pomelos; then, a gas chromatograph, a hydrogen flame ionization detector and a liquid chromatogram-ultraviolet detector is used at the same time for detection; chemical ingredients in each position of the Fujian Guanxi honey pomelos are subjected to chromatographic qualitative analysis; specific substances of cnidium lactone, limonene, alpha-linoleic acid, palmitic acid, sitosterol, oleamide and nootkatone and the like in the Fujian Guanxi honey pomelos can be accurately detected; a honey pomelo specific substance chromatographic fingerprint chromatogram is successfully built. The method provided by the invention has the advantages that the operation is simple; the qualitative detection is accurate; the detection is fast; the method belongs to a fire-new fast, accurate and simple honey pomelo origin traceability and quality identification method.

Description

technical field [0001] The invention belongs to the field of analytical chemistry chromatography. Specifically, it is a method for identifying Fujian Guanxi honey pomelo by chromatography. The method uses gas chromatography and liquid chromatography to qualitatively analyze the chemical components in Fujian Guanxi honey pomelo. Background technique [0002] Pomelo (citrus junos tanaka), also known as Wendan, is a plant of the genus Pomelo in the citrus subfamily of Rutaceae. my country is the largest producer of pomelo in the world, and its output ranks second. Our country is rich in pomelo varieties and there are many varieties (strains). At present, the main varieties are honey pomelo, Guanxi honey pomelo, Yuhuan pomelo, etc., accounting for about 80% of the country's pomelo cultivation area, with an average planting area of ​​more than 3,700 hectares. The output exceeds 12,000 tons, mainly distributed in Fujian, Zhejiang, Taiwan, Guangxi, Guangdong and other provinces. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 李雪生宋世明陈兆杰
Owner GUANGXI UNIV
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