Endothelio-aneurysm stand and its production
A technology of endothelial cells and aneurysms, applied in the field of biomedical materials, can solve problems such as limited adhesion and promote thrombus formation, and achieve the effect of preventing thrombus formation, reducing the incidence rate, and mature and feasible technical methods
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Embodiment 1
[0035] (1) Culture autologous vascular endothelial cells
[0036]Take the jugular vein from one side of the dog used for the experiment, peel off the mustard tissue around the vein under aseptic conditions, wash the blood vessel repeatedly with D-hank's solution, wash the blood in the blood vessel, tie the blood vessel at one end with surgical suture, and turn the inner wall of the blood vessel outward , the other end of the blood vessel was tied with surgical sutures, and the blood vessel segment was digested in 1% collagenase (Sigma) and 0.125% trypsin (Sigma) at 37°C for 20 minutes, then the digestion was terminated with serum, the cell suspension was sucked out, and centrifuged at 1500 rpm After 5 minutes, discard the supernatant, resuspend the cells with 80% DMEM, 20% fetal bovine serum, 20ng / ml VEGF medium, and mix 1×10 6 The cell suspension of the cell density was planted in a petri dish coated with poly-lysine and placed in 37°C 5% CO 2 Cultured in an incubator, the c...
Embodiment 2
[0048] 1. The same method as in Example 1 for culturing autologous vascular endothelial cells.
[0049] 2. Preparation of coating material and surface coating of stainless steel stent
[0050] Lactide or lactone and morpholine dione derivatives containing polyfunctional amino acids, wherein the molar ratio of the two is 1:99, are polymerized with stannous octoate as a catalyst; the reaction temperature is 50-200 ° C, and the reaction The time is 0.5 to 46 hours, and the molar ratio of the lactide or lactone of the obtained polymer to the morpholinodione derivative containing a multifunctional amino acid is 1:99; the obtained polymer uses HBr / HAc as a catalyst to remove the protective group Finally, a biodegradable polyester with reactive side groups is obtained, the reaction temperature is 10-35°C, and the reaction time is 8-80h; then the deprotected polymer and heparin are dissolved in the mixed solvent THF / H2O, The reaction is carried out with dicyclohexylcarbodiimide as a ...
Embodiment 3
[0056] Example 3 Experiment of Interventional Treatment of Aneurysms with Endothelialized Stents
[0057] The autoendothelialized aneurysm stent is uniformly compressed on the surface of the microcatheter balloon for use.
[0058] Select the same experimental dog (15-20Kg) as above, and establish the common carotid artery aneurysm model after collecting the external jugular vein (aneurysm neck: 8mm; tumor body 6×8×10mm 3 ). Three weeks after the establishment of the model, dogs with common carotid artery aneurysm were fixed on a digital subtraction angiography (DSA) bed after intravenous general anesthesia, and the 6F arterial sheath was inserted through the right femoral artery. After routine angiography showed the location and shape of the aneurysm, a 6F guiding catheter was placed at the proximal end of the parent common carotid artery. The autoendothelialized aneurysm is delivered to the aneurysm neck through a microcatheter, and the stent is released after the balloon i...
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