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This specification provides a structure for in vitro blood perfusion that can effectively remove reactive oxygen species (ROS) that cause inflammation in the blood, an in vitro blood perfusion tube including the structure and an in vitro blood purification device, a method for preparing the structure, and an in vitro blood purification method using the in vitro blood purification device, wherein the structure for in vitro blood perfusion includes cerium dioxide nanoparticles and a carrier loaded with cerium dioxide nanoparticles.