232 results about "Microtubule cytoskeleton" patented technology

Disclosed is a bio-electrospinning technique for preparing a cell-containing, oriented, continuous tubular scaffold, made of biodegradable polymer, designed for use as a nerve guide conduit (NGC) in nerve regeneration. With a coaxial spinneret, the PC-12 cell medium solution was co-electrospun into a core of tubular fibers, with PLA on the outer shell. The resulted fibers' morphology was characterized via SEM and optical microscopy, and following structural characteristics were found: 1. the larger, hollow fibers had diameters in tenth of microns and wall thicknesses around few microns, 2. an orientation in a preferred direction with the aid of a high-rotating collection device. The fluorescent PC12 cells embedded within the scaffold were cultured and nerve growth factor was added. We observed cells could not only survive the process, but also sustain their viability by undergoing differentiation process, extending neurite along the micro tubular scaffold in the desired direction. All these results demonstrate its potential application for advanced NGC.

The present invention pertains to a microencapsulated fire retardant and the uses thereof. The inventive micro-encapsulated fire retardant comprises a core containing a fire retardant and a shell of a natural microtubule, wherein the core is encapsulated by the shell. The microencapsulated fire retardant of the present invention can be composited into a certain polymeric substrate by a mixing method so as to form into a corresponding, fire-retardant polymeric composite material.

The present invention discloses analysis system and method of microbubble behavior in biomedicine ultrasonic field based on superhigh speed photograph technology. Specifically, he present invention includes proposing superhigh speed photograph system for observing the microbubbles in ultrasonic field and microscopic superhigh speed photograph system for observing single microbubble; presenting the method of analyzing the microbubble behavior in free field, near tissue and inside microtubule; and presenting the method of controlling microbubbles generation and breaking and estimating microbubble strain.

A processes for the separation and purification of microtubules of halloysite clay by separating an inlet stream of a liquid slurry of suspended clay particles flowing into a cross-flow filter into a decantate stream and a filtrate stream, the filtrate stream having a higher concentration of microtubular particles than the inlet stream. Also disclosed are subsequent preparations of novel structures and compositions of matter that include microtubules of halloysite clay.

The invention relates to translation enhancer elements that enhance translation of the gene encoding the human microtubule-associated tau protein and nucleic acid molecules that enhance translation of the gene encoding the human α-synuclein protein. The translation enhancer elements of the invention are useful in compositions and methods for identifying compounds for the prevention and / or treatment of neurodegenerative disease. The invention also includes in some aspects, vectors that include a translation enhancer element of the invention. The invention also includes the use of enhancer element containing vectors in methods to produce recombinant protein and in assays to identify compounds that modulate expression of tau protein or α-synuclein protein.

Disclosed is an ultracapacitor having electrodes containing mineral microtubules, an electrolyte between the electrodes, and a separator in the electrolyte to provide electrical insulation between the electrodes, while allowing ion flow within the electrolyte. The electrodes may be formed from a paste containing microtubules, a conductive polymer containing mineral microtubules, or an aerogel containing the mineral microtubles. The mineral microtubules may be filed with carbon, a pseudocapacitance material, or a magnetoresistive material. The mineral microtubules may also be coated with a photoconductive material.

An ultracapacitor comprising a first electrode containing mineral microtubules, a second electrode containing mineral microtubules, an electrolyte disposed between the first electrode and the second electrode, and a separator disposed in the electrolyte to provide electrical insulation between the first electrode and the second electrode, while allowing ion flow within the electrolyte. The electrodes may be formed from a paste containing mineral microtubules, or may consist essentially of a conductive polymer containing mineral microtubules, or an aerogel containing the mineral microtubules. The mineral microtubules may be filled with carbon, a pseudocapacitance material, or a magnetoresistive material. The mineral microtubules may also be coated with a photoconductive material.

Disclosed are a radiation absorptive material and more particularly a method of economically coating halloysite or other mineral tubules (including nanotubules and microtubules) with a conductive metal (Cu) in order to produce an absorptive composite material capable of providing shielding and attenuation of radio-frequency signals.

The invention discloses a thrombus extraction support device. The thrombus extraction support device comprises a thrombus extraction support, a conveying guide wire, a guiding guide wire and marking rings, and is characterized in that the thrombus extraction support is composed of a front thrombus extraction support body and a back thrombus extraction support body; the marking rings include the middle-section marking ring and the near-end marking ring, the back end of the front thrombus extraction support body is fixedly connected with the back thrombus extraction support body through the middle-section marking ring, and the front end of the front thrombus extraction support body is fixedly connected with the guiding guide wire so that vascular irritation of the front thrombus extraction support body can be effectively reduced, and vasospasm in the thrombus extraction process can be prevented; the back end of the back thrombus extraction support body is fixedly connected with the near-end marking ring, the front end of the conveying guide wire is connected with the near-end marking ring, and the back end of the conveying guide wire is connected with a microtubule so that the front thrombus extraction support body and the back thrombus extraction support body can successively carry out thrombus extraction, and thrombus can be effectively prevented from falling in blood vessels. Due to the structure, the thrombus extraction support device has the advantages that the structure is simple, the capturing performance is high, thrombus extraction is complete, vasospasm is prevented, and the blood flow recovering velocity is high.

The administration of cytotoxic agents followed by the administration of heat shock protein 90 inhibitors, such as ansamycins, has a synergistic effect on the growth inhibition of cells. This synergy occurs at doses of each cytotoxic agent that normally only causes minimal growth inhibition of cells. Such combination therapy thus allows one to use lower doses of cytotoxic agents to avoid or reduce their respective toxicity to patients without compromising their growth inhibitory effects. Thus, these combinations can be used for the treatment of an animal, preferably a mammal, that has a cell proliferative disorder, whether the cells have wild-type Rb or are Rb deficient or Rb negative. One such method, directed to treating cell proliferative disorders includes the step of administering a therapeutic effective amount of a cytotoxic agent followed by administering a therapeutic effective amount of a heat shock protein 90 inhibitor. The cytotoxic agent may be a microtubule-affecting agent, topoisomerase II inhibitor, a platinum complex, paclitaxel, or a paclitaxel derivative. The HSP90 inhibitor may be an ansamycin, radicicol or a synthetic compound that binds to the ATP-binding site of HSP90.

This invention relates to certain 5-arylpyrimidine compounds or a pharmaceutically acceptable salt thereof, and compositions containing said compounds or a pharmaceutically acceptable salt thereof, wherein said compounds are anti-cancer agents useful for the treatment of cancer in mammals. This invention further relates to a method of treating or inhibiting the growth of cancerous tumor cells and associated diseases in a mammal and further provides a method for the treatment or prevention of cancerous tumors that express multiple drug resistance (MDR) or are resistant because of MDR, in a mammal in need thereof which method comprises administering to said mammal an effective amount of said compounds or a pharmaceutically acceptable salt thereof.More specifically, the present invention relates to a method of treating or inhibiting the growth of cancerous tumor cells and associated diseases in a mammal in need thereof by promotion of microtubule polymerization which comprises administering to said mammal an effective amount of said compounds and pharmaceutically acceptable salts thereof.

The invention discloses a device and a method of assembly of a microtubule and a microsphere based on monocular microscopic vision. The device of the assembly of the microtubule and the microsphere based on the monocular microscopic vision comprises a motion platform, a manipulator, a clamping device, a translational motion platform, a microscopic vision system, a support frame and a vibration isolation platform, wherein the microscopic vision system is installed on the translational motion platform, the translational motion platform is installed on the support frame, the microtubule is installed on the manipulator through the clamping device, the microsphere is placed on the motion platform, and the support frame, the manipulator and the motion platform are installed on the vibration isolation platform. When the microtubule and the microsphere are assembled, the motion platform is adjusted so that the microsphere is enabled to enter a view of the microscopic vision system, the translational motion platform is adjusted so that an image of micropore formed in the microsphere is enabled to be clear, the microtubule is driven by the manipulator to enter the view of the microscopic vision system, the manipulator is adjusted till the tail end of the microtubule is clear, the manipulator drives the microtubule to conduct a three-dimensional motion, and the microtubule is driven to be align with and be inserted into the micropore. According to the alignment and assembly device and the method of the microtubule and the microsphere based on the monocular microscopic vision, the monocular microscopic system is utilized to achieve alignment and assembly of the microtubule and the microsphere without the need of repeated focusing of the microscopic vision system, and the application is convenient.

The invention relates to a preparation method for a coaxial micro-channel reactor and comprises the following steps: (1) selecting three polyfluortetraethylene micro tubes, wherein a micro tube with a larger inner diameter (A) is used as an outer tube and the other two with relatively small inner diameter (a1, a2) are used as inner tubes; (2) adopting epoxide resin to seal in a length of glass capillary or hollow fibre at the tube head supposed to enter micro tube A of the micro tube a1; (3) puting the two micro tubes a1and a2 in the micro tube A, wherein a1 is located in the position of the central axis of the micro tube A and the length of a1 entering the tube A is larger than a2; and utilizing epoxide resin to seal and fixe the micro tubes and get a coaxial micro channel; (4) and connecting micro tube a1 and a2 respectively to two injectors which are placed on an inject pump to form the coaxial micro-channel reactor. The invention has simple and convenient production process, and controllable micro-channel size, thereby realizing the use for producing monodisperse polymeric beads with the diameter ranging from 200 to 1000 micrometers. Furthermore, the invention has the advanthges of low cost and short consumption time.

Current approaches for treating cancer are limited, in part, by the inability of drugs to affect the poorly vascularized regions of tumors. We have found that spores of anaerobic bacteria in combination with agents which interact with microtubules can cause the destruction of both the vascular and avascular compartments of tumors. Two classes of microtubule inhibitors were found to exert markedly different effects. Some agents that inhibited microtubule synthesis, such as vinorelbine, caused rapid, massive hemorrhagic necrosis when used in combination with spores. In contrast, agents that stabilized microtubules, such as the taxane docetaxel, resulted in slow tumor regressions that killed most neoplastic cells. Remaining cells in the poorly perfused regions of tumors could be eradicated by sponzlated bacteria. Mechanistic studies showed that the microtubule destabilizers, but not the microtubule stabilizers, radically reduced blood flow to tumors, thereby enlarging the hypoxic niche in which spores could germinate. A single intravenous injection of spores plus selected microtubule-interacting agents was able to cause regressions of several tumors in the absence of excessive toxicity.

Embryogenesis from plant microspores is routinely induced with a 16-24 h temperature treatment of 32.5° C. Continuous culture at 25° C. results in pollen development. However, microspore treatment with anti-cytoskeletal agents, or protein synthesis inhibitors, at the non-inductive temperature of 25° C., can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction. Furthermore, when anti-microtubule agents (e.g. colchicine) are used, embryo induction and chromosome doubling occur simultaneously, thus generating doubled haploids, whereas heat induction generates haploids. Thus, the use of microtubule inhibitors will provide a simple one-step process to simultaneously induce embryogenesis and chromosome doubling for the production of fertile plants, thus providing minimal manipulation which will be very advantageous for genetic studies and plant breeding programs. As noted, heat shock induces haploids. A low level of chromosome doubling can be obtained by adding colchicine to microspore cultures during the heat treatment. However, the use of trifluralin with the heat treatment, to generate doubled haploid plants results in an improved recovery of fertile doubled haploid plants than previously shown in the prior art.

The administration of cytotoxic agents followed by the administration of heat shock protein 90 inhibitors, such as ansamycins, has a synergistic effect on the growth inhibition of cells. This synergy occurs at doses of each cytotoxic agent that normally only causes minimal growth inhibition of cells. Such combination therapy thus allows one to use lower doses of cytotoxic agents to avoid or reduce their respective toxicity to patients without compromising their growth inhibitory effects. Thus, these combinations can be used for the treatment of an animal, preferably a mammal, that has a cell proliferative disorder, whether the cells have wild-type Rb or are Rb deficient or Rb negative. One such method, directed to treating cell proliferative disorders includes the step of administering a therapeutic effective amount of a cytotoxic agent followed by administering a therapeutic effective amount of a heat shock protein 90 inhibitor. The cytotoxic agent may be a microtubule-affecting agent, topoisomerase II inhibitor, a platinum complex, paclitaxel, or a paclitaxel derivative. The HSP90 inhibitor may be an ansamycin, radicicol or a synthetic compound that binds to the ATP-binding site of HSP90.

Disclosed are a novel bionic hepatic tissue engineering scaffold and a interface, and producing method thereof, comprising designing a hepatic tissue engineering scaffold imitating natural hepatic lobule structure, and arranging two separate sets of micro duct structure and a interface thereof for hepatocytes and endothelial cells inside the scaffold. The micro duct interface of endothelial cells can be connected with blood vessels in the nature liver, or with the circulatory system of a bioreactor. The hepatocyte micro duct structure is used for accelerating hepatocyte diffusion when perfusing hepatocytes. A mono-layer flow passage and a interface are produced by press print method, and then pore space between two micro duct structures is formed by freeze drying, so that the porosity factor of the material is increased to make the material have certain semi-permeable membrane function. The three-dimensional scaffold and the interface thereof with hepatocyte micro duct structure and endothelial cell micro duct structure are produced by lamination bonding process.

In accordance with the invention, novel gene deletions and translocations involving chromosome 2 resulting in fusion proteins combining part of Anaplastic Lymphoma Kinase (ALK) kinase with part of a secondary protein have now been identified in human solid tumors, e.g. non-small cell lung carcinoma (NSCLC). Secondary proteins include Echinoderm Microtubule- Associated Protein-Like 4 (EML-4) and TRK- Fusion Gene (TFG). The EML4-ALK fusion protein, which retains ALK tyrosine kinase activity, was confirmed to drive the proliferation and survival of NSCLC characterized by this mutation. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ALK kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of this new fusion protein enables new methods for determining the presence of these mutant ALK kinase polypeptides in a biological sample, methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides, which are also provided by the invention.

The application discloses novel 2-alkoxyestradiol analogs which exhibit anti-proliferative properties, and methods of making and using such compounds to inhibit undesired cell proliferation and tumor growth. Additionally, methods are disclosed of treating diseases associated with undesired angiogenesis and undesired proliferation, and methods of treating infectious disease wherein the infectious agent is particularly susceptible to inhibition by agents that disrupt microtubule organization and function.

The new-type spinal cord and peripheral nerve repairing material possesses same-direction microtube type structure characteristics, and can be made into the forms of cylinder and rectangle according to the requirements. It has the following characteristics: 1. exteral surface of said material is full-closed structure, can effectively preventing internal fibre connective tissue from growing into; 2. the microtube diameter size of the material can be controlled in 30-200 micrometers, it is favorable for growth of nerve regenerative fibre; and 3. arrangement directions of microtubes are axial, and mutual parallel and uniform. it is favourable of directional extension of nerve regenerative fibre. It can be dirctly implanted for repairing spinal cord and peripheral nerve defect, also can be used as carrier of various cell implantation, can carry various nerve growth factors and nerve trunk cell, etc.

The present invention generally concerns microtubule protrusions in cancer cells, including detached cancer cells, and inhibition of the protrusions. In particular aspects, the inhibition of the protrusions interferes with attachment of the cell to a vessel wall, and in further aspects the cell is killed by forcing it to enter capillaries and be destroyed, for example by shearing. Inhibition by a variety of agents and methods is contemplated.

The invention discloses an NGF (nerve growth factor) chitosan microsphere and high-bionic stent slow releasing system, which is a cylindrical collagen and chitosan nerve stent. Chitosan microspheres buried with nerve growth factors are uniformly loaded in the stent. A preparation method of the stent includes firstly, preparing the chitosan microspheres buried with the nerve growth factors by an emulsification chemical-crosslink method; secondly, preparing the collagen and chitosan nerve stent with mutually parallel axial microtubules sample structures; and finally, compounding the chitosan microspheres onto the collagen and chitosan nerve stent via after-compounding technology and preparing the NGF chitosan microsphere and high-bionic stent slow releasing system. The stent structure is similar to a normal nerve basilar membrane structure, and the stent realizes an effect of physically guiding regeneration nerves to the greatest extent. Besides, the stent can slowly release the nerve growth factors with bioactivity for 12 weeks, can lead the nerve growth factors to be effectively released to a nerve injury portion for a long time, and accordingly achieves the purpose of promoting long nerve defect repair better and faster.

This invention relates to certain 6-[(substituted)phenyl]triazolopyrimidine compounds or pharmaceutically acceptable salts thereof, and compositions containing said compounds or pharmaceutically acceptable salts thereof, wherein said compounds are anti-cancer agents useful for the treatment of cancer in mammals. This invention further relates to a method of treating or inhibiting the growth of cancerous tumor cells and associated diseases in a mammal and further provides a method for the treatment or prevention of cancerous tumors that express multiple drug resistance (MDR) or are resistant because of MDR, in a mammal in need thereof which method comprises administering to said mammal an effective amount of said compounds or pharmaceutically acceptable salts thereof. The present invention relates to a method of treating or inhibiting the growth of cancerous tumor cells and associated diseases in a mammal in need thereof by promotion of microtubule polymerization which comprises administering to said mammal an effective amount of said compounds and pharmaceutically acceptable salts thereof.

Disclosed herein are novel benzophenone derivatives represented by formula I, a pharmaceutically acceptable salt thereof, a hydrate thereof or a solvate thereof, a pharmacological composition containing the same, and a use of the composition as therapeutic drugs. The benzophenone derivatives have an inhibition activity of microtubule formation and can be used to treat a normal proliferative state of a malignant tumor by killing the actively proliferating cells.

An immune-fluorescence labeling method of micro-pipe frame at pollen tube on gymnosperm includes fixing pollen tube of gymnosperm on fixing liquid for 40-60min, washing pollen tube, placing pollen tube in enzyme solution for carrying out enzymolysis of 30-40 min under temperature of 20-30deg.c, washing pollen tube, setting pollen tube in Triton X-100 to penetrate there for 1-3h, washing pollen tube, adding anti-beta-tubulin single clone antibody to carry out hatching for 1-3h, washing pollen tube, adding fluorescence label to carry out shielded hatching for 1-3h for making micro-pipe frame be immune-fluorescence labeled.

The invention discloses a pesticide microcapsule and a preparation method thereof. The pesticide microcapsule consists of a capsule core and a capsule wall; and the capsule core is pesticide and the capsule wall is a compound capsule wall which is formed by encapsulating natural fibers by high polymer materials. The preparation method comprises the following steps of: 1) dispersing natural fibers in liquid pesticide, and soaking to ensure that liquid pesticide is filled in microtubules of natural fibers; 2) and encapsulating the natural fibers in which pesticide is filled in the step 1) by using the high polymer materials to obtain the pesticide microcapsule. The cotton fibers which are adopted in the method have natural plant microtubules which have high hollowness, are environment-friendly, and low in price and can be obtained easily, and have the characteristics of high encapsulation capacity, low cost, sustained release and the like when used for the pesticide microcapsule.

A medical implant that delivers therapeutic via microtubes and a method of making the same is provided. In one embodiment a biologically implantable structure adapted to fit within the body of a patient is provided. This structure may have a plurality of individual microtubes in physical communication with its outer surface, the microtubes containing or carrying a therapeutic. In an alternative embodiment a method of manufacturing an implantable medical appliance is provided. This method includes placing a pliant stratum of microtubes onto a biologically implantable medical structure and then applying a therapeutic to the pliant stratum to cover or fill the microtubes.

The invention provides a laser-enhanced three-dimensional micro-region electro-deposition method. the method integrates laser irradiation into a microtubule-based micro-region electro-deposition device to obtain an improved deposition rate. Electrolyte is poured into an electrolyte chamber and a microtubule, a tip of the microtubule is close to a sample surface by the regulation of a translation stage, a droplet at the tip of the microtubule forms a meniscus on the sample surface so that the diffusion of the droplet on the sample surface is limited, and machining accuracy of electro-depositionis beneficially controlled. As the electrolyte in the microtubule is connected with the positive potential and the surface of a conductive sample is the negative potential, the electro-deposition process can occur. The laser is irradiated on the contact surface between the droplet and the sample, and a laser thermal effect improves the electron transfer efficiency in the electrolyte, acceleratesthe positive shift of the electrochemical balance potential and causes local eddy current stirring in the droplet, and therefore accelerates the electro-deposition process. According to the preset pattern, the electronic control transition stage movement is regulated, so the three-dimensional relative movement of the microtubule and the sample surface is realized.

The invention discloses a microtubule-associated protein and coding genes and application thereof. The microtubule-associated protein is protein of (a) or (b): (a) protein formed by an amino acid sequence as shown by a sequence one in a sequence table; and (b) protein derived by the sequence one, related to the inhibition of the microtubule de-polymerization activity of MCAK protein and formed ina way the amino acid sequence of the sequence one is substituted and / or deleted and / or added by one to ten amino acid residues. The protein of the invention participates in the regulation and the control of the forward end dynamics of microtubules through the mutual effect with EB1 and MCAK and stabilizes the microtubules through inhibiting the microtubule de-polymerization activity of the MCAK protein. After the coding genes of the low-interference RNA of microtubule-associated protein genes are introduced into a host, the microtubule positioning of the MCAK is seriously affected. The protein and the coding genes thereof play a great role in the medical field and the pharmaceutical field and thereby the application prospect is wide.