11 results about "Enhancer Elements" patented technology

Provided herein are novel anti-effector moiety antibodies or antigen binding domains thereof, and CARs comprising such effector moiety antigen binding domains with or without one or more enhancer elements, as well as host cells expressing the receptors and nucleic acid molecules encoding the receptors, and methods of using the same in patient-specific immunotherapy useful for the treatment of solid tumor cancers and other diseases and disorders.

The invention provides a disease risk elncRNA and risk gene identification method, equipment, a medium and a program product, and relates to the field of intelligent medical treatment. The invention provides a process SCZ-elnc. Risk elncRNA driven by a disease GWAS signal and a target gene thereof are identified by integrating the following two steps. The method comprises the following steps: (1) identifying risk elncRNA by combining risk SNP, eQTL data and enhancer elements in a genome position; and (2) predicting the gene regulated by the elncRNA through a plurality of support evidences from transcriptomics and epigenomics data.

Provided are isolated enhancer element sequences that regulate and restrict expression of a transgene, such as a therapeutic gene, to certain neuronal cell types and / or populations in the brain and CNS. Therapeutic virus vectors containing the cloned enhancer element sequences, particularly, recombinant adeno-associated virus (rAAV) vectors, and a transgene are described. The rAAV vectors, compositions and methods are useful for treating subjects afflicted with neuropsychiatric and neuropathological diseases, disorders and conditions and symptoms thereof. The vectors can be used to restore normal cellular function, e.g., by restoring expression of certain genes to the appropriate interneuron or neuron target cell populations, to address the root cause of the disease, e.g., by restoring the excitation-inhibition balance in the neuronal cell or cell population.

Provided are isolated, cloned enhancer element sequences that target certain neuronal cell types, subtypes, and / or populations in the brain and CNS. The isolated and cloned enhancer elements can target a gene of interest, such as a therapeutic gene or an effector gene, to be expressed in the certain cell types. Virus vectors containing the isolated, cloned enhancer element sequences, particularly, recombinant adeno-associated virus (rAAV) vectors, and a gene or polynucleotide to be expressed in a given neuronal cell type are described. The rAAV vectors, compositions and methods are useful for treating subjects afflicted with neuropsychiatric and neuropathological diseases, disorders and conditions and symptoms thereof.

The present invention relates to the field of plant molecular biology and provides materials and methods for modulating expression of a gene of interest in plants. In particular, the invention provides modified plant promoters or modified coding sequences having increased expression, for example, in developing spikes as well as methods for producing promoters or coding sequences having increased expression. The modified promoters comprise i) at least one binding site for an EIL3 transcription factor and / or at least one binding site for a PHD transcription factor and / or ii) one or more enhancer elements. Moreover, the present invention concerns a nucleic acid molecule encoding a functional restorer polypeptide for wheat G-type cytoplasmic male sterility comprising in the coding sequence a mutated microRNA (“miRNA”) binding site. In some embodiments, said nucleic acid molecule is operably linked to the modified promoter of the present invention.

The invention relates to the technical field of biotechnology and genetic engineering, in particular to a fungus-derived element with a transcription enhancement function and application thereof. The invention provides a polynucleotide sequence capable of enhancing the transcription function of the promoter, and when the polynucleotide sequence is added to the upstream of different promoters, the expression intensity of downstream genes mediated by the promoter can be remarkably improved. The polynucleotide sequence provided by the invention can be applied to the genetic engineering technology of fungi, the expression of a target gene is enhanced, the yield of organic acids and enzyme preparations is further improved, and the polynucleotide sequence has a relatively wide application prospect.

The disclosure relates, at least in part, to methods and compositions for improving rAAV production. The disclosure provides methods of contacting a cell culture with one or more enhancer elements to improve rAAV yield.

The present invention relates to synthetic enhancer elements that can be used to increase the expression of a gene of interest in any plant tissue. The disclosed synthetic enhancer elements can be used in a gene expression cassette comprising nucleic acid further comprising a promoter and a sequence to be expressed, wherein the sequence to be expressed has a transcription start site, and wherein the synthetic enhancer element is operably linked to the promoter and the sequence to be expressed. The present invention also relates to a modified plant or part thereof comprising a synthetic enhancer element, wherein the synthetic enhancer element is operably linked to an endogenous promoter in the modified plant or part thereof and an endogenous gene in the modified plant or part thereof, and wherein the synthetic enhancer element increases expression of a transcript or protein encoded by the endogenous gene.

The invention relates to a novel artificial enhancement subelement, an assembly and a construction method and application of the novel artificial enhancement subelement. The invention provides an artificial enhancer element capable of guiding high-level expression of a target gene as well as an assembly and application of the artificial enhancer element. Preferably, the artificial enhancer element and the assembly of the artificial enhancer element can guide the high expression level of an antibody gene and enhance the diversity of an antibody. The designed and assembled artificial enhancer element and assembly have the function of a super enhancer, can construct a targeting vector, guide high-level expression of a target gene, especially an antibody gene, and increase diversity of antibody gene expression products. Expression of luciferase in mouse myeloma cells is guided according to the artificial enhancer element and the composition thereof, but the artificial enhancer element and the composition thereof can be generally used for expression of target genes in mice, preferably expression of antibody genes.

The present invention provides a method, device, medium, and program product for identifying disease risk elncRNAs and risk genes, relating to the field of intelligent medicine. This application provides a process, SCZ-elnc, for identifying risk elncRNAs and their target genes driven by disease GWAS signals by integrating the following two steps: (1) identifying risk elncRNAs by combining risk SNPs, eQTL data, and enhancer elements in genomic locations; and (2) predicting genes regulated by elncRNAs using multiple supporting evidence from transcriptomics and epigenomic data.

The present disclosure provides DNA binding proteins engineered to bind selectively to engineered synthetic enhancer elements that are orthogonal to a host genome. Introduction of the DNA binding protein and the synthetic enhancer is used, in several embodiments, to modulate gene expression of a gene of interest without disruption or alteration of other genes within the host cell genome. In several embodiments, the DNA binding protein selected is a zinc finger protein.