6 results about "Enhancer Elements" patented technology

Provided are isolated, cloned enhancer element sequences that target certain neuronal cell types, subtypes, and / or populations in the brain and CNS. The isolated and cloned enhancer elements can target a gene of interest, such as a therapeutic gene or an effector gene, to be expressed in the certain cell types. Virus vectors containing the isolated, cloned enhancer element sequences, particularly, recombinant adeno-associated virus (rAAV) vectors, and a gene or polynucleotide to be expressed in a given neuronal cell type are described. The rAAV vectors, compositions and methods are useful for treating subjects afflicted with neuropsychiatric and neuropathological diseases, disorders and conditions and symptoms thereof.

The invention relates to the technical field of biotechnology and genetic engineering, in particular to a fungus-derived element with a transcription enhancement function and application thereof. The invention provides a polynucleotide sequence capable of enhancing the transcription function of the promoter, and when the polynucleotide sequence is added to the upstream of different promoters, the expression intensity of downstream genes mediated by the promoter can be remarkably improved. The polynucleotide sequence provided by the invention can be applied to the genetic engineering technology of fungi, the expression of a target gene is enhanced, the yield of organic acids and enzyme preparations is further improved, and the polynucleotide sequence has a relatively wide application prospect.

The disclosure relates, at least in part, to methods and compositions for improving rAAV production. The disclosure provides methods of contacting a cell culture with one or more enhancer elements to improve rAAV yield.

The present invention relates to synthetic enhancer elements that can be used to increase the expression of a gene of interest in any plant tissue. The disclosed synthetic enhancer elements can be used in a gene expression cassette comprising nucleic acid further comprising a promoter and a sequence to be expressed, wherein the sequence to be expressed has a transcription start site, and wherein the synthetic enhancer element is operably linked to the promoter and the sequence to be expressed. The present invention also relates to a modified plant or part thereof comprising a synthetic enhancer element, wherein the synthetic enhancer element is operably linked to an endogenous promoter in the modified plant or part thereof and an endogenous gene in the modified plant or part thereof, and wherein the synthetic enhancer element increases expression of a transcript or protein encoded by the endogenous gene.

The present invention provides a method, device, medium, and program product for identifying disease risk elncRNAs and risk genes, relating to the field of intelligent medicine. This application provides a process, SCZ-elnc, for identifying risk elncRNAs and their target genes driven by disease GWAS signals by integrating the following two steps: (1) identifying risk elncRNAs by combining risk SNPs, eQTL data, and enhancer elements in genomic locations; and (2) predicting genes regulated by elncRNAs using multiple supporting evidence from transcriptomics and epigenomic data.

The present disclosure provides DNA binding proteins engineered to bind selectively to engineered synthetic enhancer elements that are orthogonal to a host genome. Introduction of the DNA binding protein and the synthetic enhancer is used, in several embodiments, to modulate gene expression of a gene of interest without disruption or alteration of other genes within the host cell genome. In several embodiments, the DNA binding protein selected is a zinc finger protein.