Endotoxin adsorption medium based on magnetic chitosan microballoon and its application method
A technology of chitosan microspheres and adsorption media, applied in the field of biomedical materials, can solve the problems of small processing capacity and large loss of active ingredients, and achieve high selectivity and easy operation
Inactive Publication Date: 2012-02-15
ZHEJIANG UNIV OF TECH
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The invention discloses an endotoxin adsorption medium based on magnetic chitosan microballoon and an application method, wherein the preparation method of endotoxin adsorption medium comprises the following steps: (1) dispersing a magnetic material in an acetic acid solution, adding chitosan to prepare water phase I, transferring water phase I to liquid paraffin containing span 80, taking glutaraldehyde as a cross-linking agent, preparing chitosan microballoon by using an anti-phase suspension method; (2) dispersing magnetic chitosan microballoon in a mixed solution of dimethyl sulfoxide and sodium hydroxide, adding chloropropylene oxide for activating, washing by deionized water till neutralizing to obtain an activated product; (3) placing the activated product in an endotoxin affinity ligand solution for immobilizing to obtain the endotoxin adsorption medium based on the magnetic chitosan microballoon. The endotoxin adsorption medium of the present invention has high selectivity of endotoxin and simple operation, and can be used for removing the endotoxin in a plurality of biological products.
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Property | Measurement | Unit |
Concentration | 30.0 ~ 40.0 | g/l |
tensile | MPa | |
Particle size | Pa | |
strength | 10 |
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