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Brevendimonas vesicularis strain and application thereof in preparing immobilized microbial adsorbent

A technology of brevundimonas and adsorbents, applied in the direction of microorganism-based methods, microorganisms, fixed on/in organic carriers, etc., can solve problems affecting the growth of animals and plants, environmental damage, human hazards, etc., and achieve high efficiency and easy to recycle

Inactive Publication Date: 2016-12-07
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heavy metal pollution is special and difficult to degrade. Even if the concentration of heavy metals in sewage is very low, it may cause great damage to the environment, affect the growth of animals and plants, and cause great harm to humans due to bioaccumulation

Method used

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  • Brevendimonas vesicularis strain and application thereof in preparing immobilized microbial adsorbent
  • Brevendimonas vesicularis strain and application thereof in preparing immobilized microbial adsorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation method of immobilized microbial adsorbent:

[0017] (1) Shake 10 mL of Brevundimonas vesicularis S3CGMCC NO.11072 bacterial suspension and 5 g of chitosan in 100 mL of liquid beef extract and peptone medium for 24 hours at room temperature to grow Brevundimonas vesicularis S3 Absorbed on the surface of chitosan, the immobilized Brevundimonas S3 chitosan composite material formed by suction filtration, washing with water, and drying at 4°C in a low-temperature vacuum; the preparation method of beef extract and peptone medium is 10g of peptone and 3g of beef extract , NaCl 5g, add distilled water 1000mL, pH=7.2-7.5;

[0018] (2) Add 5 g of the obtained composite material to 100 mL of a 5wt.% sodium alginate aqueous solution and stir to mix evenly, then use a row gun to inhale 50 μL of the mixed solution each time, and add the mixed solution dropwise to a 2wt.% calcium chloride solution to make the seaweed The sodium alginate forms a gel, and the microbial...

Embodiment 2

[0021] The preparation method of immobilized microbial adsorbent:

[0022] (1) Shake 10 mL of Brevundimonas vesicularis S3CGMCC NO.11072 bacterial suspension and 10 g of chitosan in 100 mL of liquid beef extract and peptone medium for 24 hours at room temperature to grow Brevundimonas vesicularis S3 Absorbed on the surface of chitosan, the immobilized Brevundimonas S3 chitosan composite material formed by suction filtration, washing with water, and drying at 4°C in a low-temperature vacuum; the preparation method of beef extract and peptone medium is 10g of peptone and 3g of beef extract , NaCl 5g, add distilled water 1000mL, pH=7.2-7.5;

[0023] (2) Add 10 g of the obtained composite material to 100 mL of a 3wt.% sodium alginate aqueous solution and stir and mix evenly, then use a row gun to inhale 50 μL of the mixed solution each time, and add the mixed solution dropwise to a 1wt.% calcium chloride solution to make the seaweed The sodium alginate forms a gel, and the microb...

Embodiment 3

[0026] Add 3g of immobilized microbial adsorbent made in Example 2 to 100mL containing 128mg / L streptomycin and 20mg / L Zn 2+ In the solution, the pH value is 6, and after shaking in a shaking incubator at 120r / min, 30°C in the dark for 24 hours, the degradation rate of antibiotics can reach more than 60%, and the removal rate of heavy metals can reach more than 80%.

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Abstract

The invention relates to a Brevendimonas vesicularis strain and application thereof in preparing an immobilized microbial adsorbent. The application method comprises the following steps: carrying out co-culture on a Brevendimonas vesicularis S3 solution and chitosan so that the Brevendimonas vesicularis S3 grows and is adsorbed to the chitosan surface to form an immobilized Brevendimonas vesicularis S3 chitosan composite material, carrying out vacuum filtration, and carrying out low-temperature vacuum drying; adding the obtained microbial composite material into a sodium-alginate-containing water solution, uniformly stirring and mixing, dropwisely adding a calcium chloride solution so that the microbial chitosan composite material is embedded to form sodium alginate microbial chitosan microspheres, washing with water, and airing to obtain the immobilized bacterium microspheres. The Brevendimonas vesicularis S3 is collected by China General Microbiological Culture Collection Center, and the collection number is CGMCC No.11072. The immobilized bacterium microspheres can be used for treating heavy-metal-polluted industrial wastewater, have the advantages of low cost and favorable effect, and are convenient and easy to recycle.

Description

technical field [0001] The invention relates to a sewage treatment material, in particular to a strain of Brevundimonas vesicles and its application in the preparation of immobilized microbial adsorbents. Background technique [0002] The sources of heavy metal polluted wastewater are very extensive, and the main sources are industrial, agricultural and domestic sewage. According to statistics, millions of tons of toxic heavy metals are released into the environment every year around the world. Heavy metal pollution is special and difficult to degrade. Even if the concentration of heavy metals in sewage is very low, it may cause great damage to the environment, affect the growth of animals and plants, and cause great harm to humans due to bioaccumulation. How to better deal with heavy metal wastewater and recover the metals in it, while reducing the treatment operating costs and avoiding secondary pollution has become a prominent issue in heavy metal treatment. [0003] Im...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C12N11/04C02F3/34C02F1/28C12R1/01
CPCC02F1/28C02F1/285C02F1/286C02F3/34C12N11/04C12N11/10C12N1/205C12R2001/01
Inventor 杨统一顾昊晨杨芬唐玉斌陈芳艳王新刚
Owner JIANGSU UNIV OF SCI & TECH
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