Vitrification ultralow-temperature preserving method for agapanthus embryogenic callus

A technology of embryogenic callus and vitrification ultra-low temperature, applied in gardening methods, plant preservation, botanical equipment and methods, etc., can solve the problems of low survival rate, failure to survive, and difficulty in preserving plant materials

Inactive Publication Date: 2012-12-19
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, research on cryopreservation technology has been carried out on different explants of more than 200 kinds of plants. However, some plants (such as tropical plants or plants with high water content) have a low survival rate or cannot survive at all after recovery. Agapanthus belongs to the tropical Plants, whose embryogenic callus has a high water content, are plant materials that are difficult to preserve, and the cryopreservation technology of Agapanthus has not been reported yet.

Method used

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  • Vitrification ultralow-temperature preserving method for agapanthus embryogenic callus
  • Vitrification ultralow-temperature preserving method for agapanthus embryogenic callus

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Embodiment 1

[0037] 1. Experimental materials

[0038] 1.1 Experimental object: Agapanthus embryogenic callus was propagated in MS solid medium containing 1.5 mg / L picloram for 20 days at 25°C, and the obtained friable and loose embryogenic callus was used as a vitrification cryopreservation experiment Object (for the preparation method, please refer to the literature "Research on Rapid Propagation Technology of Blue Lily", Fan Xianli, 2009, Master's Degree Thesis of Shanghai Jiaotong University).

[0039] 1.2 Experimental reagents

[0040] 1.2.1 The loading solution contains 2M glycerol, 0.4M sucrose, 10mM KNO 3 MS culture medium.

[0041] 1.2.2 Vitrification solution is MS culture solution containing 30wt% glycerol, 15wt% ethylene glycol, 15wt% dimethyl sulfoxide, and 0.4M sucrose.

[0042] 1.2.3 The washing solution contains 1.2M sucrose and 10mM KNO 3 MS culture medium.

[0043] 1.2.4 The pretreatment medium is MS solid medium with sucrose content of 0.3M, 0.5M and 0.7M respective...

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Abstract

The invention relates to the field of ultralow-temperature preservation, and in particular relates to a vitrification ultralow-temperature preserving method for agapanthus embryogenic callus, after low temperature-hypertonic pretreatment, the agapanthus embryogenic callus is sequentially dehydrated in loading solution and vitrification solution; and finally, the dehydrated agapanthus embryogenic callus is added with the vitrification solution again, and then quickly put in liquid nitrogen to be preserved. The agapanthus embryogenic callus is preserved at ultralow temperature through specially designing the treatment methods, conditions and reagents of a vitrification ultralow temperature preserving process, so a technical guarantee is provided for preserving agapanthus genetic resources, a new effective method is provided for somatic embryogenetic seedlings and new product cultivating materials, and a guiding function is achieved for preserving plant resources which grow in tropical zones or contain high water contents and for completing an asexual propagation technical system.

Description

technical field [0001] The invention relates to the field of cryopreservation, in particular to a method for vitrifying cryopreservation of embryogenic callus of Agapanthus lilifolia. Background technique [0002] Agapanthus praecox ssp.orientalis, also known as 'blue lily', is a perennial rhizome flower native to southern Africa. Agapanthus has beautiful leaf shape, beautiful flower posture, elegant flower color, long flowering period, strong resistance to stress, and strong ornamental characteristics. From the use of medicinal components of its leaves and rhizomes to ornamental flowers, from garden cultivation to cut flowers Production, with high ornamental value and application value, is high-grade fresh cut flowers, potted flowers and coastal park ground covering materials in western developed countries, and is also the most expressive love flower besides roses. In order to rapidly propagate the introduced excellent germplasm resources, it is necessary to carry out the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00A01H4/00
Inventor 申晓辉李晓丹任丽张荻
Owner SHANGHAI JIAO TONG UNIV
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