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Spruce somatic embryogenesis and plant regeneration method

A technology of embryogenesis and somatic cells, applied in plant regeneration, botany equipment and methods, horticultural methods, etc., can solve problems such as difficulty in cultivating organs, difficulty in meeting production and application needs, low plant regeneration rate, etc., and achieve high reproduction coefficient, Low cost, the effect of alleviating urgent needs

Active Publication Date: 2011-03-09
NANJING FORESTRY UNIV
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AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: the purpose of the present invention is to provide a method of using immature embryos of Chinese fir trees to induce the formation of embryogenic embryonic stalks for the current Chinese fir tissue culture organ generation difficulties, low plant regeneration rate, and difficulty in meeting the needs of production and application. A method for realizing somatic embryogenesis and regenerating Chinese fir plants, in order to realize the rapid production of a large number of high-quality seedlings and meet the needs of forestry production for high-quality Chinese fir seedlings

Method used

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  • Spruce somatic embryogenesis and plant regeneration method
  • Spruce somatic embryogenesis and plant regeneration method
  • Spruce somatic embryogenesis and plant regeneration method

Examples

Experimental program
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Effect test

Embodiment 1

[0038] (1) Preparation of Chinese fir immature seed embryos

[0039] The test materials were immature embryos of the hybrid combination of Chinese fir excellent trees, collected from the seed orchards of Chinese fir clones in Shunchang and Weimin, Fujian, respectively, and repeated sampling for 2 years. The cones are collected every 7 days from the end of June to the end of August every year. The seed development stages corresponding to each seed collection time point were the developmental stages from immature embryo to mature embryo. After the cones are collected, put them in a pre-frozen ice box on site and wrap them with moist gauze to prevent water loss; then quickly put them in a 4°C refrigerator for storage for later use. Store in refrigerator at 4°C for at least one week, which is conducive to the efficient induction of ESM. Before inoculation, take the spare cones out of the refrigerator, use a scalpel and tweezers to remove the well-developed immature seeds with se...

Embodiment 2

[0081] In step (3), the 2 in the maintenance solid medium formula, 4-D is 2mg / L, 6-BA is 0.5mg / L, and the 2 in the liquid proliferation medium formula, 4-D is 0.5mg / L; In step (5), the ABA in the maturation medium formula is 8 mg / L, GA is 1 mg / L, and PEG 8000 is 200 g / L. Other each reaction step and condition are all with embodiment 1, test result shows, can reach the effect of embodiment 1.

Embodiment 3

[0083] In step (3), the 2 in the maintenance solid medium formula, 4-D is 0.5mg / L, 6-BA is 0.2mg / L, and the 2 in the liquid proliferation medium formula, 4-D is 2mg / L; In step (5), the ABA in the maturation medium formula is 3mg / L, GA is 5mg / L, and PEG 8000 is 100g / L. Other each reaction step and condition are all with embodiment 1, test result shows, test result shows, can reach the effect of embodiment 1.

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Abstract

The invention discloses a spruce somatic embryogenesis and plant regeneration method, which comprises preparation of immature seed embryo of spruce, initial induction of embryonal suspensor mass (ESM), maintenance and proliferation of the ESM, prophase maturation culture of the somatic embryo, maturation culture of the somatic embryo and germination of the somatic. The spruce plant regeneration method has the advantages of high propagation coefficient and no limitation on the propagation season and provides an efficient means for the rapid propagation of the genetically improved materials of spruce, which not only is conducive to the storage of genetic resources of spruce, but also provides a large number of genetically improved plant materials for accelerating the cultivation of the improved materials of spruce, eases urgent requirements of the society on the genetically improved nursery stock of spruce, and provides a technology for producing an improved variety of nursery stock with short period, high efficiency and low cost.

Description

technical field [0001] The present invention relates to the biological technology field of seedling propagation in forestry, in particular to a method of inducing in vitro proliferation of immature zygotic embryogenic embryonic stalk cell mass (Embryonal Suspensor Mass, ESM for short) developed into a complete body A method of embryonic and plant regeneration. Background technique [0002] Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) belongs to the genus Cunninghamia of Taxodiaceae, and is one of the endemic evergreen conifer species in my country. Chinese fir grows fast, has a straight and round stem shape, and is famous for its good material properties and beautiful wood texture. It has a wide range of uses and is an important commercial timber afforestation tree species in my country. Since the 1960s, research on the selection and breeding of Chinese fir varieties has cultivated a large number of excellent provenances, families and excellent clones for afforestatio...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈金慧周小红施季森
Owner NANJING FORESTRY UNIV
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