Phosphate dissolving bacterium and acquisition method thereof

An acquisition method and technology of phosphate-solubilizing bacteria, applied in the field of agricultural biology, can solve the problems of lack of culture medium improvement and innovation, limited discovery of novel strains, insufficient system and depth, etc., to enrich patent strain resources, enrich the screening technology system, strengthen the Effect of Phosphorus Dissolution Ability

Inactive Publication Date: 2015-07-15
HENAN UNIV OF SCI & TECH
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  • Abstract
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Problems solved by technology

Generally speaking, the wild strains used for industrial production and development are extremely single. Except for the development and application of Bacillus megaterium phospholyticus, others are far from systematic and in-depth.
As far as the screening method of phosphate-solubilizing bacteria is concerned, the method was single in the past, and the improvement and innovation of the medium were lacking, which limited the discovery of novel strains

Method used

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  • Phosphate dissolving bacterium and acquisition method thereof
  • Phosphate dissolving bacterium and acquisition method thereof
  • Phosphate dissolving bacterium and acquisition method thereof

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Embodiment Construction

[0037] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative work all belong to the scope of protection of the present invention. The experimental methods in the following examples, if no special instructions , are conventional methods.

[0038] The embodiment of the present invention provides a method for obtaining phosphate solubilizing bacteria, the method comprising the following steps:

[0039] S1. Sample collection: Collect soil samples from the sunny slope of mixed coniferous and broad-leaved forest in Tianchishan National Forest Park, Songxian County, Luoyang City, at an altitu...

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Abstract

The invention provides a phosphate dissolving bacterium and an acquisition method thereof, belonging to the field of agricultural biotechnology. The phosphate dissolving bacterium and the acquisition method thereof can enrich the genetic resource of wild phosphate dissolving bacteria and enlarge a backup library for whole-genome breeding of phosphate dissolving bacteria. The phosphate dissolving bacterium provided by the invention is named as YYF22 and is preserved in China Center for Type Culture Collection with an accession number of CCTCC No: M2015043. The colony characteristics of the phosphate dissolving bacterium are as follows: the YYF22 strain is coccobacillus with a length of 1.2 to 2.9 [mu]m and a width of 0.4 to 1.5 [mu]m; the phosphate dissolving bacterium is normally arrayed in pairs and may independently exist; the phosphate dissolving bacteria sometimes adhere to from a filose shape or chain shape; the phosphate dissolving bacterium is free of flagellum, has a capsule, and cannot move; and on an LB flat, the colony of the bacterium has a neat edge, a moist surface and low bulges, and is glossy and cream yellow.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a phosphate solubilizing bacterium and an acquisition method thereof. Background technique [0002] The content of phosphorus in plants is second only to nitrogen and potassium, and it is one of the key nutrients for plant growth and development. Phosphorus-deficient soil generally refers to the soil with soil available phosphorus (P) less than 10mg / kg. The area of ​​phosphorus-deficient soil in my country exceeds 74% of the total cultivated land area. Most of the phosphorus in the soil exists in the form of insoluble phosphate, and plants cannot directly Absorb and utilize. In order to meet the needs of production, most of the cultivated fields in my country need to supplement a large amount of phosphorus fertilizers. Due to long-term accumulation and enrichment, a series of production and ecological problems such as soil compaction and nutrient imbalance have been caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04C12R1/01
CPCC12N1/20C12N1/205C12R2001/01
Inventor 孙会忠王小东宋月芹侯小改张利霞
Owner HENAN UNIV OF SCI & TECH
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