Excised reproduction method for mountain ash

A technology of in vitro propagation and rowan, which is applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of complicated seed germination treatment technology, easy variation of gene traits, low fruit seed rate, etc., to achieve Improve growth and ornamental quality, reduce pests and diseases, and facilitate detoxification

Inactive Publication Date: 2008-01-16
NORTHEAST FORESTRY UNIVERSITY +3
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of long growth cycle, slow molding, difficult seed collection, low fruit germination rate, complicated seed germination treatment technology, low seed emerge

Method used

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Experimental program
Comparison scheme
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specific Embodiment approach 1

[0009] Specific embodiment one: present embodiment Sorbus is propagated in vitro according to the following steps: 1. Inoculate the sterilized Sorbus embryo to every liter and add 0.5~1.0mg 6-benzylaminopurine and 0.05~0.1mg naphthalene acetic acid, pH Adventitious buds were formed by culturing on MS solid medium with a value of 5.5-6.0 for 30-40 days; 2. Transfer the adventitious buds to a pH value of 0.5-1.0 mg 6-benzylaminopurine and 0.05-0.1 mg per liter Subculture on the MS solid medium of 5.5-6.0 until micro-branches are formed; 3. Cut the micro-branches or subcultured micro-branches into stem segments with a length of 1-3 cm, with axillary buds or terminal buds, Then inoculate the rootless seedlings with 0.5 to 1.0 mg of 6-benzylaminopurine and 0.05 to 0.1 mg of naphthalene acetic acid per liter and a pH value of 5.5 to 6.0 on the WPM medium and cultivate them until the length of the axillary or terminal buds is ≥ 1 cm; 4. Transfer the rootless seedlings to MS solid med...

specific Embodiment approach 2

[0011] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the rowan embryos in step one are mature rowan embryos or young rowan embryos. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0012] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 is: it also includes step 5: transplanting the regenerated plants with well-developed root system into the domestication substrate mixed by peat soil and sand at a volume ratio of 2:1 , acclimatized and cultivated under the conditions of a temperature of 25-30°C and a humidity of >80%. Other steps and parameters are the same as those in Embodiment 1.

[0013] The acclimatization and cultivation in this embodiment is to gradually increase the light time and ventilation of the regenerated plants, and the regenerated plants that have been acclimated and cultivated can be transplanted into greenhouses or greenhouses for cultivation.

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Abstract

The mountain ash in vitro propagation method relates to the propagation method of mountain ash, which resolves the deficiencies existed in the prior sowing pattern of mountain ash propagation such as long life cycle, slow forming speed, difficult seed collection, low seedling volume ratio of fruit, complex germination processing technology, low seeding ratio, low seedling yield and the variation easily occurred in the gene character. According to the in vitro propagation, the adventitious bud is firstly formed, after that the mini shoot is formed as well. And the mini shoot or the subculture mini shoot propagation is implemented for the formation of the rootless plant. After the rooting, the regenerated plant of mountain ash is obtained. The mountain ash in vitro propagation method of the invention is simple and easy to master, which is not subject to the restrictions of seasonal, climatic factors and without requirement to occupy a large amount of land. The mountain ash propagated with the method of the invention has the same gene as that of the fine parent. Thereby the unit of characters is ensured. The mountain ash propagation of the invention method has the advantages of short breeding cycle, high reproduction coefficient, high survival rate and rapid growth.

Description

technical field [0001] The present invention relates to the propagation method of rowan. Background technique [0002] Sorbus (Sorbus pohuashanensis Hendl), also known as Baihuashan Sorbus and Sorbus tree, belongs to the deciduous small tree of Rosaceae Malinae Sorbus, and is one of the precious ornamental tree species in northern my country. Sorbus has both ornamental, cold resistance, and adaptability, so it is very suitable for promotion and cultivation in northern cities with cold winters and monotonous colors. [0003] At present, in production and scientific research, rowan is propagated by sowing, which has the following disadvantages: 1. The growth cycle is long and the molding is slow; 2. Since the mother trees for seed collection are all natural wild rowan trees, and they are scattered in the forest , so the seed collection is difficult and the source is unstable; 3. The fruit emergence rate is low, only about 1%; 4. The seeds have deep dormancy characteristics, a...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 沈海龙王爱芝杨玲
Owner NORTHEAST FORESTRY UNIVERSITY
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