Aronia melanocarpa chloroplast genome and applications thereof

A technology of chloroplast genome, Aronia nigrum, applied in the field of genetics

Pending Publication Date: 2021-10-08
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current research on the chlo

Method used

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  • Aronia melanocarpa chloroplast genome and applications thereof
  • Aronia melanocarpa chloroplast genome and applications thereof
  • Aronia melanocarpa chloroplast genome and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1, Sample Total DNA Extraction

[0033] The present invention extracts the total DNA of the sample by CTAB method. The experimental steps are as follows:

[0034] Fresh blades (or refrigerated from the - 80 ° C refrigerator) were placed in a liquid nitrogen-containing mortar in a mortar containing liquid nitrogen. Take 200-400 μL of powder in a 2.0 mL centrifuge tube, 800 μL of preheated CTAB solution (0.2% β-mercaptoethanol) is added to promote lysis of cells and prevent DNA oxidation. With a whirlpool, fully mixed, it was placed in a thermostatic oven at 65 ° C for 30 minutes, and the mixed solution was reversed every 10 min. Afterwards, 400 μl of chloroform, isopentyl alcohol mixed solution (24: 1), reversed mixing 1 min, 12,000 rpm, centrifugation for 10 min. The supernatant was taken into a new 1.5 ml centrifuge tube with a pipette, and 400 μl of pre-cooling isopropyl alcohol was added, and the mixed isopropyl alcohol was added, and then pelletted in the -20 ° ...

Embodiment 2

[0036] Example 2, sequencing library construction

[0037] The total DNA sequence is randomly interrupt with the ultrasonic crusher to repair the interrupted sequence end and add A tail and sequencing joints; cutting recovery, purification; PCR amplification completion of the construction of sequencing library.

Embodiment 3

[0038] Example 3, and the data assembling chloroplast genome annotation embodiment

[0039] Using fastp software filter sequencing data obtained and to obtain clean data; application bowtie2 the comparative analysis cleandata the NCBI published Rosaceae species chloroplast genome, pick out a fragment belonging to the chloroplast genome, then reuse NOVOPlasty software These fragments splicing assembly; using GeSeq chloroplast genome analysis and annotation, and use correct tRNA result, artificial correction encoding genes and annotations rRNA encoding gene-SE tRNAscan, finally obtained a complete black fruit Aronia chloroplast genome; OGDRAW ( V1.3.1) the data finally obtained is formed chloroplast genome annotation FIG see figure 1 .

[0040] Results: chloroplast genome size of ribs Sorbus black fruit gland is 157,987bp, total GC content of 36.7%; contains one pair of inverted repeat 26,436bp size, GC content 42.5%; a small size of the single copy 19,121bp regions, GC content of 3...

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Abstract

The invention firstly discloses chloroplast complete genome information of aronia melanocarpa, the definition that the chloroplast complete genome information is applied to aronia plants, and the phylogenetic status of the aronia melanocarpa in the rosaceae. According to the research, molecular biology and phylogenomics methods are utilized, the phylogenetic relationship of aronia species is established through an aronia melanocarpa chloroplast genome. On this basis, the whole chloroplast genome is used as a super DNA bar code of the aronia and is used for identifying the variety of the aronia. Technical support is provided for introduction, protection and species identification of aronia melanocarpa germ plasm resources. The method has important significance for introduction, definition, breeding and genetic diversity evaluation, protection and utilization of aronia melanocarpa plants.

Description

Technical field [0001] The present invention belongs to the field of genetic technology, and specific to the macro gland genome and its application thereof. Background technique [0002] Aronia Melanocarpa is a rosacea adenfench (Aronia), which is also called no old raspberry, wild riverberry, and north America. Strains 1.3-2.4 meters; leaves, intervodes; gender flowers, white, umbrella fancy; fruits are pear fruit, near spherical, purple black. Its berry is rich in various functional components (such as flavonoids, anthocyanins and polyphenols, etc.), which have special effects in the treatment of cancer, diabetes, cardiovascular and cerebrovascular diseases, and obesity, so as functional food in Europe and America. And the raw materials developed by drugs are widely used in the pharmaceutical and food industry. The macarrostal ribben and the 1990s introduced our country, but also set to ornamental flowers, leaves, fruits and other values, and cold-resistant, drought resistance,...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12Q1/6869C12Q1/6895
CPCC07K14/415C12Q1/6869C12Q1/6895C12Q2535/122C12Q2537/165
Inventor 张凡王传永周艳威种昕冉陈红李云龙周婷陆小清
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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