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Method for preparing a plurality of caffeoylquinic acid monomers from honeysuckle

A technology for caffeoylquinic acid and honeysuckle, which is applied in the field of chemical substances or pharmaceutical manufacturing, can solve the problems of low purity of caffeoylquinic acid substances, difficult to scale up production of separation technology, consumption of organic solvents, etc. Effectiveness of utilization, easy promotion, and simple method

Inactive Publication Date: 2013-02-27
HUBEI CHUTIANSHU PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The invention provides a method for preparing various caffeoylquinic acid monomers from honeysuckle, which solves the problem that the purity of caffeoylquinic acid substances in the existing preparation is not high, and solves the problem that the existing technology of extracting and preparing chlorogenic acid consumes a lot of energy. Organic solvents cause environmental pollution; or it is difficult to scale up production by using more complicated separation techniques; or the extraction efficiency is not high, and the utilization rate of raw materials is low, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Raw material pretreatment steps:

[0025] Get honeysuckle dried product as raw material, after pulverizing and sieving, accurately weigh 1000g, add the aqueous solution 500ml that contains cellulase 0.50g, pectinase 0.50g, wherein the volume of aqueous solution and the mass ratio of raw material are 0.5: 1 (volume mass ratio), the mass ratio of the amount of cellulase to the raw material is 0.5: 1000 (mass ratio), the mass ratio of the mass of pectinase to the raw material is 0.5: 1000 (mass ratio), heated to 60 ° C, after 1 hour of reaction , dried to obtain the extracted raw material;

[0026] (2) Extraction step

[0027] Extract the raw materials obtained in step (1), extract with 80% ethanol, extract at 60°C, and extract 3 times at a solid-liquid ratio of 1:10, and combine the extracts;

[0028] (3) macromolecule precipitation step

[0029] Obtain concentrated solution 5000ml after the extraction solution obtained in step (2) is concentrated, the volume mass ...

Embodiment 2

[0038] (1) Raw material pretreatment steps:

[0039] Get honeysuckle dried product as raw material, after pulverizing and sieving, accurately weigh 2000g, add the aqueous solution 4000ml that contains cellulase 6.0g, pectinase 4.0g, wherein the volume of aqueous solution and the mass ratio of raw material are 2: 1 (volume mass ratio), the mass ratio of the amount of cellulase to the raw material is 3: 1000 (mass ratio), the mass ratio of the mass of pectinase to the raw material is 2: 1000 (mass ratio), heated to 30 ° C, after 3 hours of reaction , dried to obtain the extracted raw material;

[0040] (2) Extraction step

[0041] Extract the raw materials obtained in step (1), extract with 50% ethanol, extract at 90°C, and extract 4 times at a solid-liquid ratio of 1:5, and combine the extracts;

[0042] (3) macromolecule precipitation step

[0043] Obtain concentrated solution 4000ml after the extraction solution obtained in step (2) is concentrated, the volume mass ratio o...

Embodiment 3

[0052] (1) Raw material pretreatment steps:

[0053] Take honeysuckle dry product as raw material, after crushing and sieving, accurately weigh 1000g, add 1000ml of aqueous solution containing 2.0g of cellulase and 1.0g of pectinase, wherein the volume of the aqueous solution and the mass ratio of the raw material are 1: 1 (volume mass ratio), the mass ratio of the amount of cellulase to the raw material is 2: 1000 (mass ratio), the mass ratio of the mass of pectinase to the raw material is 1: 1000 (mass ratio), heated to 45 ° C, after 2 hours of reaction , dried to obtain the extracted raw material;

[0054] (2) Extraction step

[0055] Extract the raw materials obtained in step (1), extract with 70% ethanol, extract at 75°C, and extract twice at a solid-liquid ratio of 1:8, and combine the extracts;

[0056] (3) macromolecule precipitation step

[0057] Obtain concentrated solution 4000ml after the extraction solution obtained in step (2) is concentrated, the volume mass ...

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Abstract

The invention relates to a method for preparing a plurality of caffeoylquinic acid monomers from honeysuckle, and belongs to preparation methods for chemical substances or drugs. In the prior art, caffeoylquinic acid substances prepared by the existing method do not have high purity; the existing chlorogenic acid extraction and preparation technology has a disadvantage of high organic solvent consumption so as to cause environmental pollution, or adopts a complex separation technology, such that production enlarging is difficult; and extraction efficiency is not high, raw material utilization rate is low, and other problems exist. With the present invention, the problems in the prior art are solved, and high purity 3-caffeoylquinic acid, high purity 4-caffeoylquinic acid, high purity 2-caffeoylquinic acid, high purity 4,5-dicaffeoylquinic acid, high purity 3,4-dicaffeoylquinic acid and other monomer components are obtained. In addition, the project technology comprises a raw material pretreatment step, an extraction step, a macromolecule precipitation step, a macroporous resin separation step, a reversed phase column separation step, a crystallization step and the like, such that production cost is saved, production efficiency is improved, the process is simple and easily enlarged into industrialization production, and the raw material utilization rate can be greatly improved.

Description

technical field [0001] The invention belongs to a method for producing chemical substances or medicines, and in particular relates to a method for obtaining various caffeoylquinic acid monomers by using honeysuckle as a raw material, extracting, separating, and purifying. Background technique [0002] Honeysuckle, also known as honeysuckle, is a perennial semi-evergreen winding woody vine of the Lonicera family. The name "Honeysuckle" comes from "Compendium of Materia Medica". Because the honeysuckle flower is white at first, and then turns yellow, it is named honeysuckle. The medicinal material honeysuckle is Lonicera Lonicera Lonicera and the dried flower buds or flowers of the same genus of Lonicera genus Lonicera. Honeysuckle has been known as a good medicine for clearing away heat and detoxification since ancient times. It is sweet and cold in nature and fragrant, sweet and cold to clear away heat without hurting the stomach, and the fragrance is thorough and can dispe...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48
Inventor 向福叶冬三向俊叶新潮闫立新
Owner HUBEI CHUTIANSHU PHARMA
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