Sweet potato leaf extract and preparation method and use thereof
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A technology for sweet potato leaves and extracts, which is applied in the field of traditional Chinese medicine preparations and their preparation, and achieves the effects of lowering prices, widening sources and protecting life safety.
Active Publication Date: 2011-10-12
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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Problems solved by technology
However, there are few reports on the treatment of hand, foot and mouth disease (EV71) and respiratory tract infection (RSV) diseases, hepatitis B and C, liver protection and enzyme reduction with sweet potato leaves.
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Embodiment 1
[0033] Example 1: Preparation of Sweet Potato Leaf Extract
[0034] (1), using dried sweet potato leaves as raw material, add water 8 times the weight of sweet potato leaves, extract with water at 95°C for 20 minutes, combine the extracts, and filter;
[0035] (2), above-mentioned filtrate is left standstill at low temperature, suction filtration;
[0036] (3), the filtrate gained in step (2) is concentrated after recovery;
[0037] (4), the D101 type macroporous resin is applied to the concentrated solution obtained in step (3), and the sample volume and resin volume ratio are 1: 2~4; then first use 3 times of column volume water elution, discard the eluent; Then eluted with 35% ethanol of 5 times column volume, and the eluate was concentrated and dried to obtain the sweet potato leaf extract.
Embodiment 2
[0038] Example 2: Preparation of Sweet Potato Leaf Extract
[0039] The steps of this example are the same as those of Example 1, the difference is that in step (1), dry sweet potato leaves are used as raw materials, water 12 times the weight of sweet potato leaves is added, water is extracted at 98° C. for 20 minutes, the extracts are combined, and filtered.
Embodiment 3
[0040] Example 3: Preparation of Sweet Potato Leaf Extract
[0041] The steps of this embodiment are the same as those of Example 1, the difference is that in step (1), dry sweet potato leaves are used as raw materials, water 10 times the weight of sweet potato leaves is added, water is extracted at 100° C. for 20 minutes, the extracts are combined, and filtered.
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Abstract
The invention provides a sweet potato leaf extract and a preparation method and use thereof and belongs to the technical field of Chinese medicine preparations and preparation methods. The sweet potato leaf extract comprises the following active components in percentage: 3.2 to 7.2 percent of 4,5-dicaffeoylquinic acid, 8.3 to 38.1 percent of 3,5-dicaffeoylquinic acid, and 4.6 to 11.2 percent of 3,4-dicaffeoylquinic acid. The preparation method comprises: leaching sweet potato leaves in water, mixing extract, and filtering; and standing at a low temperature, filtering under vacuum, recovering,concentrating, loading concentrate on macroporous resin column, eluting with water and ethanol in turn, concentrating ethanol eluent, drying and obtaining the sweet potato leaf extract. The sweet potato leaf extract can treat hand-foot-and-mouth disease (EV71), respiratory syncytial virus(RSV) disease, hepatitis B and hepatitis and can protect liver and reduce enzymes. The invention has the advantages that: the sweet potato leaf extract can be used as substitutes for the conventional expensive medicines; and the preparation process is simple.
Description
technical field [0001] The invention belongs to the technical field of traditional Chinese medicine preparations and preparation methods thereof, and in particular relates to a sweet potato leaf extract, a preparation method and application thereof. Background technique [0002] Hand-foot-mouth disease (HPMD) is a common infectious disease caused by a variety of enteroviruses, and is a legal Class C infectious disease in my country. There are more than 20 types (types) of enteroviruses that cause hand, foot and mouth disease. Coxsackievirus types 16, 4, 5, 9, and 10 of group A, types 2 and 5 of group B, and enterovirus type 71 are all Among the more common pathogens of HFMD, Coxsackievirus A16 (CoxA16) and enterovirus 71 (EV71) are the most common. [0003] my country is a high-incidence area of viral hepatitis, among which chronic hepatitis B has attracted special attention because of its gradual development, difficulty in control, poor prognosis and complicated pathogen...
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