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Method for simultaneously determining content of five chemical components in chrysanthemum medicinal materials

A technology for chemical components and medicinal materials, applied in the field of simultaneous determination of five chemical components in chrysanthemum medicinal materials, to achieve comprehensive quality characteristics, high sensitivity and good separation.

Inactive Publication Date: 2019-08-16
BEIJING CHENGYI INVESTMENT CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few reports on the chemical composition and quality of chrysanthemum medicinal materials. It is necessary to establish a method for determining the content of index components in chrysanthemum medicinal materials to reflect the quality characteristics of chrysanthemum medicinal materials comprehensively.

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  • Method for simultaneously determining content of five chemical components in chrysanthemum medicinal materials
  • Method for simultaneously determining content of five chemical components in chrysanthemum medicinal materials
  • Method for simultaneously determining content of five chemical components in chrysanthemum medicinal materials

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Embodiment 1

[0058] 1 Medicinal materials, reagents and instruments

[0059] 1.1 Medicinal materials and reagents

[0060] The medicinal material of Fubaiju (dried medicinal material in that year) was collected in Hubei Province, and the specific information is shown in Table 1 (the numbers and letters contained in the batch number in Table 1 have no special meaning, and only represent different batches). ). Chlorogenic acid, luteolin-7-O-β-D-glucoside, luteolin-7-O-β-D-glucuronide, 3,5-dicaffeoylquinic acid, apigenin- 7-O-β-D-glucoside reference substances are all from China National Institutes for Food and Drug Control, with a purity of >98.0%. Acetonitrile and methanol were chromatographically pure.

[0061] Table 1 Fubaiju sample collection information table

[0062]

[0063] 1.2 Instruments

[0064] Agilent 1200 high performance liquid chromatograph (Agilent Corporation of the United States), ultraviolet detector; AUW120D electronic balance (0.01mg, Shimadzu Corporation of Jap...

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Abstract

The invention relates to the technical field of effective component determination, and in particular relates to a method for simultaneously determining content of five chemical components in chrysanthemum medicinal materials. By adoption of the method provided by the invention, the content of chlorogenic acid, luteolin-7-O-beta-D-glucoside, luteolin-7-O-beta-D-glucuronide, 3, 5-dicaffeoylquinic acid and apigenin-7-O-beta-D-glucoside can be measured simultaneously. According to the method provided by the invention, the separation degree is high, the separation of all the components can achievethe baseline separation, the linear range is large, the sensitivity is high, and the repeatability is high; the sample addition recovery rate of the five chemical components ranges from 95% to 105% ina sample addition recovery test, which all meet the specification. Therefore, the method provided by the invention provides an effective detection means for the chrysanthemum medicinal materials withthe five chemical components as quality detection indexes, and the quality characteristics of the chrysanthemum medicinal materials can be comprehensively reflected.

Description

technical field [0001] The invention relates to the technical field of effective component determination, in particular to a method for simultaneously determining the contents of five chemical components in chrysanthemum medicinal materials. Background technique [0002] Chrysanthemum is the dry head of Chrysanthemum chrysanthemum (Chrysanthemum morifolium Ramat.), which tastes sweet, bitter, and slightly cold. , Fengreganmao, headache and dizziness embolism. The main chemical components of chrysanthemum are flavonoids, volatile oils, organic acids, polysaccharides and amino acids. At present, there are few reports on the chemical composition and quality of chrysanthemum medicinal materials. It is necessary to establish a method for determining the content of index components in chrysanthemum medicinal materials to reflect the quality characteristics of chrysanthemum medicinal materials comprehensively. Contents of the invention [0003] The object of the present inventi...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027G01N2030/062
Inventor 汪豪程雪翔
Owner BEIJING CHENGYI INVESTMENT CO LTD
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